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There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5′-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5′-hexachlorofluorescein-CE phosphoramidite (HEX) and 5′-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5′-end. Labeling the 3′-end of an oligo with fluorescein can be achieved using 3′-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
6-FAM phosphoramidite, 100 µmol | sc-363596 | 100 µmol | $132.00 |