Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
γ1-Adaptin Double Nickase Plasmid (h): sc-403986-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- γ1-Adaptin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- γ1-Adaptin Double Nickase Plasmid (h) and γ1-Adaptin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AP1G1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: γ1-Adaptin Antibody (F-10): sc-398867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γ1-Adaptin Double Nickase Plasmid (h) | sc-403986-NIC | 20 µg | $410.00 | |||
γ1-Adaptin Double Nickase Plasmid (h2) | sc-403986-NIC-2 | 20 µg | $410.00 |
AP1G1 encodes the human γ1-adaptin subunit of the AP-1 adaptor protein complex, a central component of clathrin-mediated vesicular trafficking at the trans-Golgi network and endosomes. γ1-adaptin helps couple cargo sorting signals to clathrin coat assembly, supporting formation of transport carriers that regulate receptor recycling, lysosomal delivery, and polarized membrane protein distribution. Through its roles in endosomal sorting and intracellular transport, AP1G1 influences signaling receptor homeostasis and membrane composition, processes frequently interrogated in studies of neurobiology, immunity, and cellular stress responses. Dysregulation of adaptor complex function and endosomal trafficking pathways has been associated with developmental and neurological phenotypes and can modulate susceptibility to pathogen entry and replication, making AP1G1 a useful node for mechanistic pathway research.
γ1-Adaptin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AP1G1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AP1G1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AP1G1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AP1G1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.