ZNF83 Activators
Chemical activators of ZNF83 employ various cellular mechanisms to modulate its activity. Zinc Chloride, by providing zinc ions, directly influences the structural integrity of ZNF83, which relies on these ions for the stabilization of its zinc finger motifs that interact with DNA. The enhanced stability of these motifs facilitates the DNA-binding process, which is central to the protein's function.
On a different front, Forskolin and Dibutyryl-cAMP elevate the levels of cAMP within the cell, which in turn activates protein kinase A (PKA). PKA is known for its role in phosphorylating various proteins, including ZNF83, thus leading to its activation. Similarly, Ionomycin and A23187 (Calcimycin) increase intracellular calcium concentrations, triggering calcium/calmodulin-dependent protein kinases that can phosphorylate ZNF83. Thapsigargin works to raise cytosolic calcium by disrupting the endoplasmic reticulum's calcium storage, which also results in the activation of calcium-dependent kinases that may target ZNF83. PMA (Phorbol 12-Myristate 13-Acetate) follows a different pathway by activating protein kinase C (PKC), a kinase known to phosphorylate a broad spectrum of proteins, including ZNF83. Calyculin A and Okadaic Acid maintain the phosphorylation state of ZNF83 by inhibiting protein phosphatases, which normally dephosphorylate proteins, effectively keeping ZNF83 in an active state. LY294002, while inhibiting PI3K, can lead to the activation of other signaling pathways that intersect with those modulating ZNF83 activity.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc Chloride can provide zinc ions that are essential for the functional structure of zinc finger proteins like ZNF83. These ions stabilize the finger motifs that directly interact with DNA, thereby enhancing the DNA-binding capability and functional activation of ZNF83. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP serves as a cAMP analog that activates PKA. The activation of PKA can lead to the phosphorylation of ZNF83, resulting in its functional activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can lead to the activation of calcium/calmodulin-dependent protein kinases (CaMKs). Activated CaMKs can phosphorylate ZNF83 as part of their target profile, leading to the functional activation of ZNF83. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 (Calcimycin) is a calcium ionophore that elevates intracellular calcium levels, potentially activating calcium-dependent kinases that could phosphorylate and activate ZNF83. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin disrupts calcium storage within the endoplasmic reticulum, leading to an increase in cytosolic calcium levels. This can activate calcium-dependent kinases, which may target ZNF83 for phosphorylation, leading to its activation. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which is known to phosphorylate a wide range of substrates, including zinc finger proteins. This phosphorylation can lead to the functional activation of ZNF83. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A inhibits protein phosphatases, which prevents the dephosphorylation of proteins, potentially keeping ZNF83 in an activated state by maintaining its phosphorylation levels. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid also inhibits protein phosphatases, promoting the phosphorylation state of proteins. This inhibition can result in the sustained activation of ZNF83 by preventing its dephosphorylation. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
LY294002 is a PI3K inhibitor that can lead to compensatory activation of alternative signaling pathways that may converge on the activation of ZNF83. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I serves as a PKC inhibitor that can lead to compensatory reactions within the cell, potentially leading to the activation of signaling cascades that phosphorylate and activate ZNF83. | ||||||