ZNF559 Activators
ZNF559 can initiate a cascade of biochemical events leading to its activation through various signaling pathways. Forskolin, by directly stimulating adenylate cyclase, raises cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates target proteins, which may include transcription factors that enhance the activity of ZNF559. Similarly, IBMX, by inhibiting phosphodiesterases, prevents cAMP degradation, maintaining PKA activity at a level that supports the phosphorylation and subsequent activation of ZNF559. The cAMP analog 8-Br-cAMP bypasses upstream signaling events to activate PKA and thus could also lead to ZNF559 activation. Additionally, PMA and 4-α-Phorbol activate protein kinase C (PKC), which may phosphorylate and activate proteins within the ZNF559 regulatory network, influencing its DNA binding affinity and transcriptional activity.
Calcium ionophores such as ionomycin and A23187 increase intracellular calcium levels, which may activate signaling pathways involving calcineurin or calmodulin-dependent kinase that could influence ZNF559 activity. Calyculin A and okadaic acid, by inhibiting protein phosphatases PP1 and PP2A, keep proteins in their phosphorylated state, which may include regulatory proteins that modulate ZNF559 activity. Cantharidin also inhibits these phosphatases, potentially leading to a similar outcome. Anisomycin, through its role in activating stress-activated protein kinases, may also contribute to the activation of ZNF559 if it is involved in stress response pathways. Lastly, Bisindolylmaleimide I (BIM), a PKC inhibitor, may under certain conditions paradoxically activate specific PKC isoforms, which in turn could activate ZNF559 through atypical PKC pathways that influence gene expression.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $260.00 $350.00 $500.00 | 34 | |
IBMX acts as a non-selective inhibitor of phosphodiesterases, enzymes that degrade cAMP. By preventing cAMP degradation, IBMX indirectly increases PKA activity, which can phosphorylate and thereby activate ZNF559 through enhanced transcription factor function. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate calcineurin and subsequently NFAT (nuclear factor of activated T-cells). NFAT may enhance the activity of ZNF559 by promoting cooperative binding to DNA or by recruiting coactivators to ZNF559 target gene promoters. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 is another calcium ionophore that raises intracellular calcium concentrations, potentially activating signaling cascades involving calmodulin-dependent kinase (CaMK), which could then influence the activity of ZNF559 by modulating the transcriptional machinery it interacts with. | ||||||
8-Bromoadenosine 3′,5′-cyclic monophosphate | 23583-48-4 | sc-217493B sc-217493 sc-217493A sc-217493C sc-217493D | 25 mg 50 mg 100 mg 250 mg 500 mg | $108.00 $169.00 $295.00 $561.00 $835.00 | 2 | |
8-Br-cAMP is a cell-permeable cAMP analog that activates PKA. Activated PKA can then phosphorylate substrates that may interact with ZNF559, leading to its functional activation by promoting its binding to DNA or interaction with other transcriptional regulators. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases PP1 and PP2A. By inhibiting these phosphatases, it indirectly maintains proteins in a phosphorylated state, which can enhance the activity of ZNF559 if it is regulated by phosphorylation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A, like okadaic acid, inhibits protein phosphatases, leading to increased phosphorylation levels of cellular proteins. This could contribute to the activation of ZNF559 if it is subject to regulation by phosphorylation that affects its transcriptional activity. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can also activate stress-activated protein kinases (SAPKs). SAPKs can phosphorylate transcription factors and other proteins, potentially enhancing ZNF559 activity if it is part of stress response signaling pathways. | ||||||
Cantharidin | 56-25-7 | sc-201321 sc-201321A | 25 mg 100 mg | $89.00 $279.00 | 6 | |
Cantharidin inhibits protein phosphatases PP1 and PP2A, similar to okadaic acid. This inhibition could lead to increased phosphorylation and activation of ZNF559 if it is regulated by dephosphorylation. | ||||||
Phorbol | 17673-25-5 | sc-253267 | 5 mg | $270.00 | 1 | |
4-α-Phorbol is a PKC activator, similar to PMA. It promotes the activation of PKC, which can phosphorylate proteins involved in the regulation of ZNF559, thereby potentially enhancing its transcriptional activation capacity. | ||||||