ZFP62 Inhibitors
Chemical inhibitors of ZFP62 can interfere with its function by preventing its phosphorylation, a modification often necessary for its activation. Forskolin, a direct adenylate cyclase activator, raises intracellular cAMP levels, which typically leads to the activation of PKA. However, in the context of inhibition, the absence of Forskolin would mean that this pathway is not engaged, thereby preventing the phosphorylation and subsequent activation of ZFP62 by PKA. Similarly, 8-Br-cAMP, a cAMP analog, and Dibutyryl-cAMP also activate PKA which in turn can phosphorylate ZFP62. Without these molecules, the activation route of PKA and the phosphorylation of ZFP62 are not facilitated. IBMX, by inhibiting phosphodiesterases, increases levels of cAMP and cGMP, which would normally lead to PKA or PKG activation and then phosphorylation of ZFP62. Without IBMX, the degradation of cAMP and cGMP by phosphodiesterases would not be impeded, resulting in lower activation of PKA or PKG and less phosphorylation of ZFP62.
Other inhibitors such as PMA, which activates PKC, could affect ZFP62 by phosphorylating it. Without PMA, PKC is less active and thus ZFP62 phosphorylation is reduced. Calyculin A and Okadaic Acid, both inhibitors of protein phosphatases, would, in their absence, allow the dephosphorylation of ZFP62, keeping it in a less active state. Anisomycin, which activates stress-activated protein kinases, would not be able to promote the phosphorylation of ZFP62 in the face of environmental stress without its presence. Zaprinast, a PDE5 inhibitor, leads to increased cGMP levels and subsequent PKG activation which then phosphorylates ZFP62. Without Zaprinast, cGMP levels would not be elevated, leading to reduced activation of PKG and thus less phosphorylation of ZFP62. Lastly, Hyperforin activates TRPC6 channels causing an influx of Ca2+ ions and activation of calcium-dependent kinases, which could phosphorylate ZFP62; without Hyperforin, this influx and subsequent activation would not occur, leading to reduced phosphorylation and activation of ZFP62.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly activates adenylate cyclase, leading to an increase in intracellular levels of cAMP, which in turn can activate cAMP-dependent protein kinase A (PKA). PKA phosphorylation can activate ZFP62 by modifying its conformation or interaction with other proteins or DNA, thus enhancing its functional activity. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $97.00 $224.00 | 30 | |
8-Br-cAMP, a cAMP analog, activates PKA. Activation of PKA can phosphorylate specific serine or threonine residues on ZFP62, which can lead to an increase in ZFP62 activity. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
IBMX is a non-selective inhibitor of phosphodiesterases (PDEs), which degrade cAMP and cGMP. By inhibiting PDEs, IBMX raises the levels of cAMP and cGMP, leading to activation of PKA and PKG, respectively. PKA or PKG can then phosphorylate ZFP62, resulting in its activation. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
Phorbol 12-myristate 13-acetate (PMA) is an activator of protein kinase C (PKC). PKC can phosphorylate ZFP62, which may alter its conformation, localization, or interaction with other proteins, and thereby increase its activity. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable cAMP analog that activates PKA. PKA activation can lead to the phosphorylation of ZFP62, thus functionally activating the protein. | ||||||
Fusicoccin | 20108-30-9 | sc-200754 sc-200754A sc-200754B | 1 mg 5 mg 10 mg | $408.00 $2040.00 $4080.00 | 7 | |
Fusicoccin leads to activation of H+-ATPase by stabilizing its interaction with 14-3-3 proteins. This can cause changes in cellular ion gradients and pH, which may indirectly activate kinases or other signaling molecules that phosphorylate and activate ZFP62. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A is an inhibitor of protein phosphatases 1 and 2A. Inhibition of these phosphatases can result in increased phosphorylation of proteins, including ZFP62, leading to its activation through prevention of dephosphorylation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Similar to Calyculin A, Okadaic Acid inhibits protein phosphatases PP1 and PP2A. This can lead to sustained phosphorylation and consequent activation of ZFP62, as the dephosphorylation process is impeded. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can activate stress-activated protein kinases (SAPKs). These kinases can phosphorylate and activate ZFP62 by modulating its stress response functions. | ||||||
Zaprinast (M&B 22948) | 37762-06-4 | sc-201206 sc-201206A | 25 mg 100 mg | $103.00 $245.00 | 8 | |
Zaprinast inhibits phosphodiesterase 5 (PDE5), leading to an increase in cGMP levels. Elevated cGMP can activate PKG, which may then phosphorylate and activate ZFP62. | ||||||