Chemical activators of ZFP316 can initiate a series of biochemical events leading to its functional activation. Phorbol 12-myristate 13-acetate directly stimulates protein kinase C (PKC), which can phosphorylate ZFP316, thereby activating it. This phosphorylation by PKC represents a common post-translational modification that regulates protein function. Forskolin, through its ability to increase intracellular cAMP levels, subsequently activates protein kinase A (PKA). Activated PKA has the capacity to phosphorylate ZFP316, which is a process that would result in the protein's activation. Similarly, Isoproterenol interacts with beta-adrenergic receptors to also raise cAMP levels and consequently activate PKA, following the same activation trajectory as Forskolin.
Ionomycin works by raising intracellular calcium levels, which activates calmodulin-dependent kinases that can phosphorylate ZFP316. Epidermal Growth Factor (EGF) engages the MAPK/ERK pathway, leading to a cascade of phosphorylation events, which can directly involve ZFP316 as a substrate, resulting in its activation. The oxidative agent Hydrogen Peroxide can create conditions that activate kinases capable of phosphorylating ZFP316. Inhibition of protein phosphatases by Calyculin A and Okadaic Acid results in sustained phosphorylation, which can cause the activation of ZFP316 through a state of increased phosphorylation. Anisomycin activates the JNK pathway, another kinase pathway, which can lead to the phosphorylation and subsequent activation of ZFP316. Histamine, through its effects on intracellular calcium pathways, can also activate PKC, again promoting the phosphorylation and activation of ZFP316. Ouabain's inhibition of Na⁺/K⁺-ATPase may alter ion balance to activate kinases that phosphorylate ZFP316. Furthermore, the cAMP analog 8-Br-cAMP activates PKA, which then can phosphorylate and activate ZFP316, echoing the activation mechanism of Forskolin and Isoproterenol. Each of these chemicals, through their unique interactions with intracellular signaling pathways and enzymes, can ultimately converge on the activation of ZFP316.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Activates protein kinase C (PKC) which can phosphorylate ZFP316 resulting in its activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Increases intracellular calcium, activating calmodulin-dependent kinases that could phosphorylate ZFP316, causing its activation. | ||||||
Hydrogen Peroxide | 7722-84-1 | sc-203336 sc-203336A sc-203336B | 100 ml 500 ml 3.8 L | $31.00 $61.00 $95.00 | 28 | |
Acts as an oxidizing agent, potentially creating an environment that activates kinases which phosphorylate ZFP316. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $28.00 $38.00 | 5 | |
Interacts with beta-adrenergic receptors, increases cAMP, and activates PKA, which can phosphorylate ZFP316, resulting in activation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Inhibits protein phosphatases leading to sustained phosphorylation, which could result in the activation of ZFP316 through phosphorylation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Activates the JNK pathway, which can lead to the phosphorylation and activation of ZFP316. | ||||||
Histamine, free base | 51-45-6 | sc-204000 sc-204000A sc-204000B | 1 g 5 g 25 g | $94.00 $283.00 $988.00 | 7 | |
Through its action on intracellular calcium pathways, can activate PKC, which may phosphorylate and activate ZFP316. | ||||||
Ouabain-d3 (Major) | sc-478417 | 1 mg | $516.00 | |||
Inhibits Na⁺/K⁺-ATPase, altering ion balance and possibly activating kinases that could phosphorylate ZFP316. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $126.00 $328.00 | 30 | |
A cAMP analog that activates PKA, leading to the phosphorylation and activation of ZFP316. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Inhibits protein phosphatases, maintaining phosphorylation state of proteins, which could lead to activation of ZFP316 through increased phosphorylation. | ||||||