YFP Inhibitors
YFP inhibitors represent a class of compounds designed to selectively target the yellow fluorescent protein (YFP), a widely utilized biomarker in molecular and cell biology studies. These inhibitors aim to modulate the fluorescence properties or stability of YFP within cellular environments. YFP, found in the green fluorescent protein (GFP), serves as a valuable tool for visualizing protein localization, protein-protein interactions, and monitoring cellular processes in live cells due to its inherent fluorescence. The development of YFP inhibitors has been pursued to manipulate YFP's fluorescence or structural characteristics, expanding the toolkit available for experimental manipulation and investigation in biological research.
Compounds categorized as YFP inhibitors possess diverse chemical structures and mechanisms to alter YFP fluorescence or stability. These inhibitors are designed to interact with specific regions of the YFP protein, affecting its fluorescent properties, photostability, or folding dynamics. Their chemical diversity includes small molecules, peptides, or other entities tailored to disrupt YFP fluorescence without compromising cellular viability or altering other cellular processes.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Rifampicin | 13292-46-1 | sc-200910 sc-200910A sc-200910B sc-200910C | 1 g 5 g 100 g 250 g | $95.00 $322.00 $663.00 $1438.00 | 6 | |
Rifampicin is an antibiotic that can inhibit RNA synthesis by binding to the DNA-dependent RNA polymerase in bacteria. This action could potentially decrease YFP expression if the YFP gene is under the control of a bacterial promoter. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $73.00 $238.00 $717.00 $2522.00 $21420.00 | 53 | |
Actinomycin D intercalates into DNA and inhibits RNA polymerase activity. By blocking RNA synthesis, it may decrease the expression of YFP, particularly if the YFP construct is actively transcribed. | ||||||
α-Amanitin | 23109-05-9 | sc-202440 sc-202440A | 1 mg 5 mg | $260.00 $1029.00 | 26 | |
α-Amanitin is a toxin that inhibits RNA polymerase II in eukaryotes. Since the YFP gene is transcribed by RNA polymerase II in eukaryotic cells, α-Amanitin can potentially reduce YFP expression by hindering the transcription process. | ||||||
Fluorouracil | 51-21-8 | sc-29060 sc-29060A | 1 g 5 g | $36.00 $149.00 | 11 | |
5-Fluorouracil is a pyrimidine analog that can be incorporated into RNA, leading to errors in RNA processing and function. This compound may decrease the stability and translation efficiency of YFP mRNA, reducing YFP protein expression. | ||||||
Doxorubicin | 23214-92-8 | sc-280681 sc-280681A | 1 mg 5 mg | $173.00 $418.00 | 43 | |
Doxorubicin intercalates into DNA and can disrupt the function of topoisomerase II, an enzyme important for DNA transcription and replication. By affecting these cellular processes, Doxorubicin might reduce YFP expression. | ||||||
Camptothecin | 7689-03-4 | sc-200871 sc-200871A sc-200871B | 50 mg 250 mg 100 mg | $57.00 $182.00 $92.00 | 21 | |
Camptothecin is a topoisomerase I inhibitor, preventing the religation of DNA strands and leading to DNA damage. This could result in cell cycle arrest and decreased YFP expression due to disrupted cellular functions. | ||||||
Cycloheximide | 66-81-9 | sc-3508B sc-3508 sc-3508A | 100 mg 1 g 5 g | $40.00 $82.00 $256.00 | 127 | |
Cycloheximide inhibits eukaryotic protein synthesis by interfering with the translocation step in protein synthesis. This action can lead to a rapid decrease in YFP protein levels, as newly synthesized YFP is not replaced when existing YFP proteins degrade. | ||||||
Chloramphenicol | 56-75-7 | sc-3594 | 25 g | $53.00 | 10 | |
Chloramphenicol inhibits bacterial protein synthesis by binding to the 50S ribosomal subunit. This antibiotic could decrease YFP expression in bacterial cells by blocking the translation of YFP mRNA. | ||||||
Tetracycline | 60-54-8 | sc-205858 sc-205858A sc-205858B sc-205858C sc-205858D | 10 g 25 g 100 g 500 g 1 kg | $62.00 $92.00 $265.00 $409.00 $622.00 | 6 | |
Tetracycline binds to the 30S ribosomal subunit in bacteria, inhibiting protein synthesis. If YFP is expressed in a bacterial system, Tetracycline could reduce YFP levels by preventing its translation. | ||||||
Puromycin | 53-79-2 | sc-205821 sc-205821A | 10 mg 25 mg | $163.00 $316.00 | 436 | |
Puromycin disrupts protein synthesis in both eukaryotic and prokaryotic cells by causing premature chain termination during translation. This compound can potentially decrease YFP expression by hindering the synthesis of the YFP protein. | ||||||