Vgl-1 Activators
Vgl-1 Activators are chemical compounds that enhance the functional activity of Vgl-1 through various cellular mechanisms. Dibutyryl-cAMP (db-cAMP), a synthetic analog of cAMP that can passively diffuse into cells, raises intracellular cAMP levels, leading to the activation of protein kinase A (PKA). This activation can result in the phosphorylation of proteins that interact with Vgl-1, enhancing its interaction with transcriptional co-activators and increasing its transcriptional activity. Similarly, Forskolin, a diterpene that activates adenylate cyclase, boosts cAMP levels and thereby activates PKA. The phosphorylation cascade that follows may enhance Vgl-1's interaction with TEAD transcription factors, augmenting the transcription of target genes.
Other compounds like Okadaic acid and MG132 act by inhibiting the dephosphorylation of proteins and the degradation of protein regulators, respectively. Okadaic acid, a potent inhibitor of protein phosphatases PP1 and PP2A, sustains the phosphorylation state of proteins, enhancing Vgl-1's association with the transcriptional machinery. MG132, by inhibiting the proteasome, can increase the levels of proteins within the cell, including those involved in pathways with Vgl-1 or that directly interact with Vgl-1, leading to an increase in Vgl-1 mediated transcriptional activity.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylate cyclase, increasing intracellular cAMP levels and activating PKA. PKA can phosphorylate substrates that support Vgl-1’s interaction with TEAD transcription factors, enhancing the transcription of genes that Vgl-1 regulates. | ||||||
Lithium | 7439-93-2 | sc-252954 | 50 g | $214.00 | ||
Lithium chloride inhibits GSK-3β, which can lead to the stabilization and nuclear localization of β-catenin. β-catenin interacts with transcription factors such as TCF/LEF which could synergize with Vgl-1 in the regulation of gene expression, thereby enhancing Vgl-1’s functional role in transcription. | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $88.00 $342.00 | 284 | |
SB203580 is a p38 MAPK inhibitor that could lead to altered transcription factor dynamics, supporting Vgl-1’s role in the transcription of specific target genes, possibly by affecting cofactors that interact with Vgl-1 in the regulation of gene expression. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG can inhibit DNA methyltransferases, leading to reduced methylation of CpG islands near gene promoters. This could increase the accessibility of transcription factor binding sites for Vgl-1, enhancing its role in transcriptional regulation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can activate calmodulin-dependent kinase, which may phosphorylate substrates that promote Vgl-1’s role in transcriptional activation in concert with TEAD transcription factors. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
5-Azacytidine is a DNA methyltransferase inhibitor that can lead to the demethylation of genes and enhance their expression. This demethylation can improve the transcriptional activity of Vgl-1 by increasing the accessibility of its target DNA sequences. | ||||||
Ademetionine | 29908-03-0 | sc-278677 sc-278677A | 100 mg 1 g | $180.00 $655.00 | 2 | |
SAM serves as a methyl donor in various methylation reactions, which can indirectly affect gene expression. SAM may modulate the methylation status of genes co-regulated by Vgl-1, potentially enhancing Vgl-1's transcriptional activity by altering the expression of its target genes or interacting partners. | ||||||