SPINDLY Inhibitors
Chemical inhibitors of SPINDLY play a significant role in modulating its function by targeting different aspects of the protein's activity. Triclosan operates by inhibiting the enoyl-acyl carrier protein reductase enzyme which is vital for the fatty acid synthesis required for SPINDLY's post-translational modification. This disruption in fatty acid synthesis impedes the proper modification and functionality of SPINDLY. Salicylic Acid exerts its inhibitory effect by competing with UDP-GlcNAc, the sugar donor substrate necessary for SPINDLY's O-GlcNAc modification activity, effectively blocking the enzyme's access to its required substrate. Alloxan and BADGP both inhibit SPINDLY by mimicking the structure of UDP-GlcNAc, the natural substrate, thus preventing the glycosylation of target proteins by SPINDLY. Streptozotocin and Acetohexamide, through their structural similarities to glucose, interfere with SPINDLY's enzymatic glycosyltransferase activity, while Azaserine and 6-Diazo-5-oxo-L-norleucine (DON) reduce UDP-GlcNAc levels, indirectly hindering SPINDLY's O-GlcNAc transferase activity by depleting the substrate pool.
Further impacting SPINDLY's function are inhibitors that disrupt glycosylation processes. Tunicamycin blocks the formation of dolichol-linked oligosaccharides, essential precursors for N-linked glycosylation, thus affecting SPINDLY's glycosylation status and functionality. Swainsonine and Castanospermine, by inhibiting mannosidase II and glucosidases respectively, impede the glycoprotein processing that SPINDLY relies on for its proper function. Similarly, Deoxynojirimycin's inhibition of glucosidases responsible for glycoprotein processing results in an impaired glycosylation state of SPINDLY. Each of these chemical inhibitors, by targeting specific biochemical pathways, enzymatic activities, or glycosylation processes, can alter the functional state of SPINDLY, demonstrating the complexity and precision of chemical regulation of protein activity.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Triclosan | 3380-34-5 | sc-220326 sc-220326A | 10 g 100 g | $138.00 $400.00 | ||
Triclosan inhibits the enoyl-acyl carrier protein reductase enzyme involved in fatty acid synthesis, which is necessary for the post-translational modification of SPINDLY. Inhibition of this enzyme disrupts the proper modification and functioning of SPINDLY. | ||||||
Salicylic acid | 69-72-7 | sc-203374 sc-203374A sc-203374B | 100 g 500 g 1 kg | $46.00 $92.00 $117.00 | 3 | |
Salicylic Acid, known for its role in plant defense, can inhibit O-linked N-acetylglucosamine transferases (OGTs) such as SPINDLY, by competing with the sugar donor substrate UDP-GlcNAc, which is necessary for SPINDLY's O-GlcNAc modification activity. | ||||||
Alloxan monohydrate | 2244-11-3 | sc-254940 | 10 g | $53.00 | ||
Alloxan selectively inhibits O-linked N-acetylglucosamine transferases (OGTs), by occupying the UDP-GlcNAc binding site, thereby preventing SPINDLY from performing its glycosyltransferase activity. | ||||||
Streptozotocin (U-9889) | 18883-66-4 | sc-200719 sc-200719A | 1 g 5 g | $110.00 $510.00 | 152 | |
Streptozotocin is a glucosamine-nitrosourea compound that can inhibit O-GlcNAc transferases by acting as a glucose analog and interfering with the enzymatic activity of SPINDLY, thus inhibiting its function. | ||||||
Azaserine | 115-02-6 | sc-29063 sc-29063A | 50 mg 250 mg | $306.00 $906.00 | 15 | |
Azaserine acts as a competitive inhibitor of glutamine-utilizing enzymes, which can lead to a reduction in the UDP-GlcNAc levels, the substrate for SPINDLY, indirectly inhibiting its O-GlcNAc transferase activity. | ||||||
6-Diazo-5-oxo-L-norleucine | 157-03-9 | sc-227078 sc-227078A sc-227078B sc-227078C | 5 mg 25 mg 100 mg 250 mg | $82.00 $285.00 $908.00 $2152.00 | ||
6-Diazo-5-oxo-L-norleucine (DON) inhibits glutamine-dependent enzymes, decreasing the availability of UDP-GlcNAc, the donor substrate for SPINDLY, and consequently inhibiting its enzymatic activity. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $169.00 $299.00 | 66 | |
Tunicamycin inhibits N-linked glycosylation by blocking the formation of dolichol-linked oligosaccharides. As glycosylation is crucial for the proper function of SPINDLY, this inhibition can disrupt its activity. | ||||||
Swainsonine | 72741-87-8 | sc-201362 sc-201362C sc-201362A sc-201362D sc-201362B | 1 mg 2 mg 5 mg 10 mg 25 mg | $135.00 $246.00 $619.00 $799.00 $1796.00 | 6 | |
Swainsonine inhibits mannosidase II, an enzyme involved in glycoprotein processing, which can impact the glycosylation status and function of SPINDLY, as it requires proper glycosylation for its activity. | ||||||
Castanospermine | 79831-76-8 | sc-201358 sc-201358A | 100 mg 500 mg | $180.00 $620.00 | 10 | |
Castanospermine is a potent inhibitor of glucosidases, enzymes involved in glycoprotein processing. Inhibiting these enzymes can impair the glycosylation and thus the function of SPINDLY. | ||||||
Deoxynojirimycin | 19130-96-2 | sc-201369 sc-201369A | 1 mg 5 mg | $72.00 $142.00 | ||
Deoxynojirimycin inhibits glucosidases that are responsible for processing glycoproteins; thus, by inhibiting these enzymes, the glycosylation and function of SPINDLY can be indirectly inhibited. | ||||||