Skint3 Activators
Chemical activators of Skint3 exploit various intracellular signaling pathways to induce its activation. Phorbol 12-myristate 13-acetate, a potent protein kinase C (PKC) activator, initiates a cascade of phosphorylation events by activating PKC, which in turn can trigger the activation of Skint3. Similarly, bryostatin 1 engages PKC to phosphorylate downstream proteins that regulate Skint3. Mezerein, like the aforementioned compounds, also activates PKC, and through this activation, it promotes phosphorylation events that may influence Skint3 activity. Ionomycin, on the other hand, raises intracellular calcium levels, thereby activating calcium-dependent kinases capable of phosphorylating proteins that interact with Skint3, leading to its activation. Likewise, thapsigargin indirectly causes an increase in cytosolic calcium by inhibiting the SERCA pump, which can result in the activation of Skint3 through calcium-dependent kinases.
In addition to the PKC pathway, Skint3 is also modulated by the cAMP-dependent pathway. Forskolin, by activating adenylate cyclase, increases cAMP levels in the cell, which activates protein kinase A (PKA). PKA can phosphorylate various regulatory proteins that associate with Skint3, resulting in its activation. Dibutyryl-cAMP, a cAMP analog, similarly activates PKA, leading to phosphorylation and activation of Skint3. Okadaic acid and calyculin A, both protein phosphatase inhibitors, lead to an accumulation of phosphorylated proteins within the cell, which can influence the activation state of Skint3. Anisomycin activates the JNK signaling pathway, culminating in the activation of transcription factors and regulatory proteins that can modulate Skint3. Lastly, teleocidin and ingenol, both inducers of PKC, can stimulate the phosphorylation of proteins within the Skint3 signaling pathway, thereby promoting its activation. Each chemical, through its unique mechanism, ensures that Skint3 is phosphorylated and activated, influencing its functional state within the cell.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
Phorbol 12-myristate 13-acetate activates protein kinase C (PKC), which then can activate Skint3 by phosphorylating downstream proteins that interact directly with Skint3, leading to its functional activation. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylate cyclase, increasing cAMP levels, which in turn activates PKA. Activated PKA can phosphorylate regulatory proteins that interact with Skint3, resulting in the activation of Skint3. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calcium-dependent protein kinases. These kinases are capable of phosphorylating proteins that associate with Skint3, thus activating Skint3. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid inhibits protein phosphatases PP1 and PP2A, leading to increased phosphorylation of cellular proteins. This increase in phosphorylation can result in the activation of Skint3 through enhanced phosphorylation of associated regulatory proteins. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin activates the JNK signaling pathway, which leads to the activation of transcription factors and regulatory proteins that can associate with Skint3, thereby promoting the activation of Skint3. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl-cAMP is a cAMP analog that activates PKA. PKA then phosphorylates cellular proteins that can lead to the activation of Skint3 by altering its interaction with other intracellular signaling molecules. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin inhibits SERCA, causing an increase in cytosolic calcium levels, which can induce the activation of calcium-dependent kinases that then may lead to the activation of Skint3 by phosphorylating proteins that regulate Skint3 activity. | ||||||
Bryostatin 1 | 83314-01-6 | sc-201407 | 10 µg | $240.00 | 9 | |
Bryostatin 1 binds to and activates PKC, and this activation can lead to the phosphorylation of proteins involved in the signaling pathways that regulate Skint3, resulting in the activation of Skint3. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A inhibits protein phosphatases, leading to an accumulation of phosphorylated proteins within the cell. This can result in the activation of Skint3 due to the enhanced phosphorylation of proteins that are involved in Skint3 signaling. | ||||||
Ingenol | 30220-46-3 | sc-202184 sc-202184A sc-202184B sc-202184C | 1 mg 5 mg 10 mg 25 mg | $69.00 $305.00 $446.00 $1120.00 | 1 | |
Ingenol activates PKC, which can lead to the phosphorylation and subsequent activation of Skint3 by modulating the phosphorylation status of proteins that interact with or regulate Skint3. | ||||||