NGEP Activators
Chemical activators of NGEP can initiate a cascade of intracellular events leading to the protein's activation through various signaling pathways. Phorbol 12-myristate 13-acetate (PMA) is a potent activator of protein kinase C (PKC), which can directly phosphorylate NGEP, resulting in its activation. This is a critical step as PKC plays a central role in the regulation of proteins through phosphorylation. Similarly, 4-α-Phorbol, another activator of PKC, can also promote the phosphorylation and consequent activation of NGEP. In parallel, calcium ionophores like ionomycin and A23187 (Calcimycin) increase the intracellular calcium concentration, which is a known activator of PKC. As PKC is stimulated, it can then target NGEP for activation. Calcium chloride functions to raise intracellular calcium levels as well, thereby potentially triggering the same pathway that culminates in NGEP activation.
Isoproterenol, functioning as a beta-adrenergic agonist, activates adenylate cyclase, which results in increased cyclic AMP (cAMP) levels within the cell. This increase in cAMP can activate protein kinase A (PKA), which may then phosphorylate NGEP. Forskolin also acts on the same adenylate cyclase pathway, enhancing cAMP levels and thereby activating PKA, which is another kinase that can target NGEP. Dibutyryl cAMP, a permeable analog of cAMP, bypasses upstream receptors and directly activates PKA, leading to potential phosphorylation of NGEP. Okadaic acid, by inhibiting protein phosphatases that normally dephosphorylate proteins, may result in the sustained activation of NGEP due to decreased dephosphorylation rates. Anisomycin, through the activation of stress-activated protein kinases like JNK, can contribute to the phosphorylation state of NGEP. Epidermal Growth Factor (EGF) can activate its receptor, leading to a signaling cascade that includes the activation of PKC, which in turn could phosphorylate NGEP. Bryostatin 1, known for its modulatory effect on PKC, can under certain conditions activate PKC and therefore increase the phosphorylation and activation of NGEP.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA directly activates protein kinase C (PKC), which in turn can activate NGEP through phosphorylation. PKC is known to phosphorylate a wide array of proteins, and phosphorylation is a common mechanism for activating membrane proteins like NGEP. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylate cyclase, increasing intracellular cAMP levels, which can lead to activation of PKA. PKA, or protein kinase A, can phosphorylate and thereby activate a variety of proteins, potentially including NGEP, if NGEP is a substrate for PKA phosphorylation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium concentrations. Elevated intracellular calcium can activate calcium-dependent protein kinases, such as PKC, which could then phosphorylate and activate NGEP. | ||||||
Calcium chloride anhydrous | 10043-52-4 | sc-207392 sc-207392A | 100 g 500 g | $65.00 $262.00 | 1 | |
Calcium chloride raises intracellular calcium levels, which can activate calcium-sensitive signaling pathways. This could lead to the activation of calcium-dependent protein kinases, such as PKC, that may phosphorylate and activate NGEP. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is another calcium ionophore that increases intracellular calcium levels. Similar to ionomycin, it can activate PKC, which may then phosphorylate and activate NGEP. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $27.00 $37.00 | 5 | |
Isoproterenol is a beta-adrenergic agonist that can activate adenylate cyclase leading to increased cAMP and activation of PKA. PKA could phosphorylate and activate NGEP as part of downstream signaling. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
Dibutyryl cAMP is a cell-permeable cAMP analog. It activates PKA, which can then phosphorylate potential substrates including NGEP, leading to its activation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid inhibits protein phosphatases 1 and 2A, leading to increased phosphorylation levels of proteins due to reduced dephosphorylation. This could result in the maintained phosphorylation and activation of NGEP if NGEP's activity is regulated by phosphorylation. | ||||||
Phorbol | 17673-25-5 | sc-253267 | 5 mg | $270.00 | 1 | |
4-α-Phorbol is a PKC activator. It can activate NGEP by promoting PKC to phosphorylate it, leading to functional activation of the protein. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin activates stress-activated protein kinases, such as JNK, which could lead to the phosphorylation and activation of NGEP if it is a substrate for these kinases. | ||||||