HMG20A Inhibitors
Chemical inhibitors of High Mobility Group 20A (HMG20A) can serve as tools to dissect the protein's role in chromatin architecture and gene regulation. Chelerythrine, for instance, inhibits protein kinase C, which is a key modulator of protein function through phosphorylation. When the activity of protein kinase C is inhibited, proteins like HMG20A, which rely on phosphorylation states to interact with chromatin and regulate gene expression, may have altered functionality. In a similar manner, Trichostatin A, as an inhibitor of histone deacetylases (HDACs), can alter the acetylation patterns of histones. Since HMG20A operates within the context of chromatin modification, changes in histone acetylation can disrupt the interaction of HMG20A with these proteins, affecting its chromatin remodeling activities. Mithramycin A binds to specific DNA sequences rich in guanine and cytosine, possibly preventing HMG20A from accessing its DNA-binding domain, thus impeding its ability to regulate transcription.
Other chemical inhibitors target the transcription and replication machinery, which indirectly influence HMG20A function. For example, DRB targets positive transcription elongation factor b, which is necessary for the transcription elongation process that HMG20A might regulate. Inhibition of this factor can restrict HMG20A's role in transcriptional elongation. Rocaglamide, by inhibiting eukaryotic initiation factor 4A, can hinder the translation of proteins that are part of the transcriptional machinery HMG20A interacts with. Similarly, Triptolide and α-Amanitin directly inhibit RNA polymerase II, thus blocking the transcription of genes where HMG20A is likely to be involved. Olivomycin A and Actinomycin D bind to DNA and obstruct RNA polymerase, which can block HMG20A's chromatin-related functions. Etoposide and Camptothecin inhibit topoisomerases II and I, respectively, which are crucial for DNA replication and transcription, processes that are essential for HMG20A's role in gene expression. Harmane's inhibition of several kinases can also affect the phosphorylation of proteins that interact with HMG20A, thereby influencing its function in transcription regulation. Each of these chemicals, by its action, can influence the biological activity of HMG20A within the cell.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Chelerythrine | 34316-15-9 | sc-507380 | 100 mg | $540.00 | ||
Chelerythrine is known to inhibit protein kinase C (PKC). As HMG20A interacts with the chromatin structure and is involved in transcription regulation, PKC inhibition can lead to altered phosphorylation states of transcription factors and chromatin-associated proteins, which could result in reduced functional recruitment of HMG20A to chromatin. | ||||||
Trichostatin A | 58880-19-6 | sc-3511 sc-3511A sc-3511B sc-3511C sc-3511D | 1 mg 5 mg 10 mg 25 mg 50 mg | $152.00 $479.00 $632.00 $1223.00 $2132.00 | 33 | |
Trichostatin A is an inhibitor of histone deacetylases (HDACs). HMG20A functions in the context of chromatin modification, and the inhibition of HDACs can alter the acetylation pattern of histones, potentially disrupting the interaction of HMG20A with these modified histones and inhibiting its function in chromatin remodeling. | ||||||
Mithramycin A | 18378-89-7 | sc-200909 | 1 mg | $55.00 | 6 | |
Mithramycin A binds to G-C rich DNA sequences and could inhibit HMG20A function by obstructing its DNA-binding domain from interacting with chromatin, as HMG20A is known to bind to such regions in the DNA. This would inhibit the protein's ability to regulate transcription. | ||||||
DRB | 53-85-0 | sc-200581 sc-200581A sc-200581B sc-200581C | 10 mg 50 mg 100 mg 250 mg | $43.00 $189.00 $316.00 $663.00 | 6 | |
5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) inhibits positive transcription elongation factor b (P-TEFb), which is essential for the transcriptional elongation phase. As HMG20A is involved in transcriptional regulation, inhibiting P-TEFb would indirectly inhibit the role of HMG20A in the elongation phase of transcription. | ||||||
Rocaglamide | 84573-16-0 | sc-203241 sc-203241A sc-203241B sc-203241C sc-203241D | 100 µg 1 mg 5 mg 10 mg 25 mg | $275.00 $474.00 $1639.00 $2497.00 $5344.00 | 4 | |
Rocaglamide inhibits eukaryotic initiation factor 4A (eIF4A), which is crucial for the initiation of translation. By inhibiting this factor, rocaglamide can indirectly inhibit the function of HMG20A by preventing the translation of key proteins that HMG20A may regulate or interact with in transcription complexes. | ||||||
Triptolide | 38748-32-2 | sc-200122 sc-200122A | 1 mg 5 mg | $90.00 $204.00 | 13 | |
Triptolide inhibits RNA polymerase II activity, which is essential for mRNA synthesis. By inhibiting this polymerase, the transcription of genes regulated by HMG20A can be inhibited, which in turn would functionally inhibit HMG20A's role in gene expression. | ||||||
α-Amanitin | 23109-05-9 | sc-202440 sc-202440A | 1 mg 5 mg | $269.00 $1050.00 | 26 | |
α-Amanitin is a potent inhibitor of RNA polymerase II. By blocking the action of this enzyme, α-Amanitin effectively inhibits transcription, potentially disrupting the transcriptional regulatory functions in which HMG20A is involved. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $74.00 $243.00 $731.00 $2572.00 $21848.00 | 53 | |
Actinomycin D intercalates into DNA, inhibiting RNA polymerase and thus transcription. By blocking transcription, it would inhibit the ability of HMG20A to participate in the regulation of gene expression. | ||||||
Etoposide (VP-16) | 33419-42-0 | sc-3512B sc-3512 sc-3512A | 10 mg 100 mg 500 mg | $51.00 $231.00 $523.00 | 63 | |
Etoposide inhibits topoisomerase II, which is essential for DNA replication and transcription. By inhibiting this enzyme, Etoposide can indirectly inhibit the function of HMG20A by preventing the transcription and replication processes in which HMG20A is implicated. | ||||||
Camptothecin | 7689-03-4 | sc-200871 sc-200871A sc-200871B | 50 mg 250 mg 100 mg | $58.00 $186.00 $94.00 | 21 | |
Camptothecin inhibits topoisomerase I, disrupting DNA replication and transcription processes. As HMG20A is involved in the regulation of gene expression, the inhibition of topoisomerase I can indirectly inhibit the ability of HMG20A to interact with chromatin and regulate transcription. | ||||||