FYCO1 Inhibitors
Chemical inhibitors of FYCO1 target various aspects of the autophagy pathway to functionally inhibit this protein's activity. Wortmannin and 3-Methyladenine are both inhibitors of phosphoinositide 3-kinases (PI3K), which play a crucial role in the initiation of autophagy. By blocking PI3Ks, these chemicals can prevent the formation of autophagosomes, leading to a decreased need for FYCO1, whose role is to assist in autophagosome trafficking. Similarly, SAR405, a specific inhibitor of the Vps34 PIK3C3 kinase, disrupts FYCO1 function by interfering with autophagy and endosomal trafficking. Spautin-1, by inhibiting ubiquitin-specific peptidases USP10 and USP13, destabilizes proteins related to autophagy, which can reduce FYCO1's activity in autophagosome processing. Bafilomycin A1 and Chloroquine function by increasing the pH in lysosomes, impairing autophagosome-lysosome fusion, a key step in the autophagic process where FYCO1 is involved.
Vinblastine and Nocodazole inhibit FYCO1 by disrupting microtubule assembly and dynamics, essential for the transport of autophagosomes to which FYCO1 contributes. Vinblastine impedes the assembly, while Nocodazole depolymerizes microtubules, each leading to an obstruction of FYCO1's role in autophagosome motility. Contrarily, Paclitaxel stabilizes microtubules to an extent that it also hinders their dynamics, and as a result, FYCO1's ability to mediate autophagosome transport is compromised. Hydroxychloroquine, much like Chloroquine, affects the autophagosome-lysosome fusion by altering lysosomal pH and function, which in turn impacts FYCO1's role in this process. Lastly, E-64d inhibits cysteine proteases within lysosomes, which are vital for the breakdown of autophagic contents. This inhibition can impede the maturation of autophagosomes, thus indirectly inhibiting FYCO1 activity in autophagosome maturation.
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Items 1 to 10 of 12 total
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Wortmannin | 19545-26-7 | sc-3505 sc-3505A sc-3505B | 1 mg 5 mg 20 mg | $66.00 $219.00 $417.00 | 97 | |
Wortmannin is a potent inhibitor of phosphoinositide 3-kinases (PI3K), which are upstream of autophagy-related pathways where FYCO1 functions. Inhibition of PI3K can lead to reduced autophagosome formation, potentially inhibiting FYCO1's role in autophagosome trafficking. | ||||||
Autophagy Inhibitor, 3-MA | 5142-23-4 | sc-205596 sc-205596A | 50 mg 500 mg | $56.00 $256.00 | 113 | |
3-Methyladenine is known to inhibit PI3K enzymes, specifically those involved in the initial steps of autophagy. As FYCO1 is implicated in the maturation and trafficking of autophagosomes, the inhibition of the autophagy initiation process can functionally inhibit FYCO1. | ||||||
Spautin-1 | 1262888-28-7 | sc-507306 | 10 mg | $165.00 | ||
Spautin-1 is an inhibitor of ubiquitin-specific peptidase 10 (USP10) and USP13, which are involved in the deubiquitination of proteins related to autophagy. By inhibiting these, Spautin-1 can lead to the destabilization of autophagy-related proteins, thereby indirectly inhibiting FYCO1 function. | ||||||
SAR405 | 1523406-39-4 | sc-507416 | 1 mg | $125.00 | ||
SAR405 is a potent and specific inhibitor of the Vps34 PIK3C3 kinase, which is involved in the regulation of autophagy and endosomal trafficking. As FYCO1 is associated with these processes, inhibition of Vps34 can indirectly inhibit FYCO1 activity. | ||||||
Bafilomycin A1 | 88899-55-2 | sc-201550 sc-201550A sc-201550B sc-201550C | 100 µg 1 mg 5 mg 10 mg | $96.00 $250.00 $750.00 $1428.00 | 280 | |
Bafilomycin A1 is a specific inhibitor of the vacuolar-type H+-ATPase (V-ATPase). By inhibiting V-ATPase, it prevents the acidification of autophagosomes, thus impairing autophagosome-lysosome fusion, a step necessary for FYCO1-mediated cargo transport within cells. | ||||||
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $68.00 | 2 | |
Chloroquine is a lysosomotropic agent that raises the pH in lysosomes, affecting their function. It is known to inhibit the fusion between autophagosomes and lysosomes, thereby indirectly inhibiting the function of FYCO1 in autophagosome processing. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is a chemical inhibitor of PI3Ks. By inhibiting PI3Ks, it blocks the autophagy pathway upstream, which can reduce the autophagic flux and thereby decrease FYCO1's functional role in autophagosome trafficking. | ||||||
Vinblastine | 865-21-4 | sc-491749 sc-491749A sc-491749B sc-491749C sc-491749D | 10 mg 50 mg 100 mg 500 mg 1 g | $100.00 $230.00 $450.00 $1715.00 $2900.00 | 4 | |
Vinblastine disrupts microtubule assembly, which is essential for FYCO1-mediated transport of autophagosomes along microtubules. By destabilizing microtubules, vinblastine can impair FYCO1's function. | ||||||
Taxol | 33069-62-4 | sc-201439D sc-201439 sc-201439A sc-201439E sc-201439B sc-201439C | 1 mg 5 mg 25 mg 100 mg 250 mg 1 g | $40.00 $73.00 $217.00 $242.00 $724.00 $1196.00 | 39 | |
Paclitaxel stabilizes microtubules and can inhibit their dynamics. This can interfere with the microtubule-dependent transport of autophagosomes, a process in which FYCO1 is involved, leading to its functional inhibition. | ||||||
Nocodazole | 31430-18-9 | sc-3518B sc-3518 sc-3518C sc-3518A | 5 mg 10 mg 25 mg 50 mg | $58.00 $83.00 $140.00 $242.00 | 38 | |
Nocodazole is a microtubule-depolymerizing agent that can disrupt microtubule networks. Since FYCO1 is implicated in autophagosome movement along microtubules, nocodazole can indirectly inhibit FYCO1's activity through microtubule depolymerization. | ||||||