Ex 495-570 nm Green

Calcein-AM is a cell-permeable fluorescent dye that exhibits remarkable sensitivity to intracellular esterase activity. Upon entering live cells, it is hydrolyzed to produce a highly fluorescent calcein, which emits light in the 495-570 nm range. This transformation is indicative of viable cells, as only metabolically active cells can convert the non-fluorescent precursor. The dye's ability to indicate cell viability and its low toxicity make it a valuable tool for studying cellular dynamics and membrane integrity.

Propidium Iodide is a fluorescent dye that selectively intercalates into nucleic acids, emitting light in the 495-570 nm range. Its unique property lies in its inability to penetrate intact cell membranes, making it a reliable marker for assessing cell membrane integrity. When bound to DNA, it exhibits enhanced fluorescence, allowing for precise detection of dead or damaged cells. This selective binding mechanism highlights its role in distinguishing between live and compromised cellular states.

Nile Red is a lipophilic fluorescent dye that exhibits strong emission in the 495-570 nm range, particularly when interacting with hydrophobic environments. Its unique ability to partition into lipid-rich structures allows it to selectively stain intracellular lipid droplets, revealing dynamic changes in cellular lipid metabolism. The dye's fluorescence intensity is influenced by the polarity of its surroundings, making it a valuable tool for studying lipid dynamics and cellular compartmentalization.

2',7'-Dichlorofluorescein diacetate is a fluorescent compound that exhibits vibrant emission in the 495-570 nm range, particularly in environments with varying pH levels. Its unique ester groups facilitate cellular uptake, where it is hydrolyzed to release the fluorescent moiety. This transformation allows for real-time monitoring of cellular processes, as the dye's fluorescence is sensitive to changes in the microenvironment, enabling insights into metabolic activity and cellular health.

JC-1 iodide is a fluorescent dye that exhibits distinct emission characteristics in the 495-570 nm range, particularly in response to changes in mitochondrial membrane potential. Its unique ability to form J-aggregates at higher potentials leads to a shift in fluorescence, providing insights into cellular energy states. This property allows for the assessment of mitochondrial function and dynamics, making it a valuable tool for studying cellular bioenergetics and apoptosis.

FlAsH-EDT2 is a fluorescent probe that operates within the 495-570 nm range, characterized by its selective binding to specific peptide sequences containing cysteine residues. This interaction facilitates the formation of a stable complex, resulting in enhanced fluorescence. The probe's unique reactivity with thiol groups allows for real-time monitoring of protein dynamics and localization, providing insights into cellular processes and molecular interactions in live cells.

Tetramethylrhodamine isothiocyanate mixed isomers exhibit a vibrant fluorescence in the 495-570 nm range, attributed to their unique structural configuration. These isomers engage in specific interactions with nucleophiles, particularly thiols, leading to the formation of covalent bonds. This reactivity enhances their stability and fluorescence intensity, making them suitable for probing dynamic molecular environments. Their distinct photophysical properties allow for effective tracking of molecular interactions in various experimental setups.

BCECF/AM is a fluorescent probe that exhibits excitation and emission in the 495-570 nm range, characterized by its ability to undergo de-esterification in cellular environments. This process enhances its fluorescence, allowing for real-time monitoring of pH changes within live cells. The compound's unique interaction with protons alters its spectral properties, making it a valuable tool for studying intracellular dynamics and environmental shifts. Its sensitivity to pH variations enables precise tracking of cellular processes.

Phenol Red is a pH indicator that exhibits distinct color changes across a specific pH range, reflecting its unique molecular structure. Its behavior as a weak acid allows it to engage in reversible protonation, influencing its spectral characteristics. The compound's hydrophobic regions facilitate interactions with lipid membranes, while its aromatic rings contribute to π-π stacking with other molecules. This versatility makes it a useful marker for studying dynamic chemical environments.

2′,7′-Dichlorodihydrofluorescein Diacetate is a fluorescent probe that exhibits strong emission in the 495-570 nm range upon deacetylation. Its unique structure allows for selective interactions with reactive oxygen species, leading to enhanced fluorescence in oxidative environments. The compound's diacetate groups enhance membrane permeability, facilitating cellular uptake. Its distinct photophysical properties enable real-time monitoring of cellular processes, making it a valuable tool in fluorescence microscopy.