DNAH5 Inhibitors
Chemical inhibitors of DNAH5 target its ATPase activity, which is crucial for its function in the assembly of dynein arms and ciliary movement. Aurintricarboxylic acid can inhibit this activity by binding to the ATP-binding sites of DNAH5, preventing the necessary hydrolysis of ATP for motor function. Similarly, Vanadate, as a phosphate analog, can bind to the ATPase domain, blocking ATP hydrolysis and thereby inhibiting the motor activity essential for ciliary beat. Emodin, by inhibiting protein kinase activity, disrupts the phosphorylation state of DNAH5, which is necessary for its function, leading to a decrease in its activity. Beryllium sulfate could also interfere with DNAH5's function through its interaction with phosphate groups, potentially binding to the ATP-binding site and disrupting motor function.
Other inhibitors affect the cellular environment and resources that DNAH5 requires to function. Oligomycin, for instance, by inhibiting ATP synthase, reduces the overall cellular ATP levels, indirectly limiting the ATP-dependent motor functions of DNAH5. In a similar vein, sodium azide inhibits cytochrome c oxidase, also contributing to the depletion of cellular ATP and hence indirectly affecting DNAH5's ATPase activity. ADP competes with ATP for the ATPase domain, and an increase in ADP concentration can inhibit DNAH5 by preventing ATP from binding to the ATPase domain, which is necessary for its function. Ruthenium Red and Thapsigargin, by altering calcium signaling, can indirectly affect the function of DNAH5, which may be regulated by calcium-dependent processes. Alsterpaullone affects ciliary assembly by inhibiting cyclin-dependent kinases, which could lead to a decrease in the activity of DNAH5 due to its role in ciliary movement. Monastrol alters microtubule dynamics, indirectly affecting DNAH5 which is associated with microtubules in cilia. Finally, Mitoxantrone disrupts DNA replication and transcription, indirectly affecting the expression and assembly of ciliary components and, as a consequence, the function of DNAH5.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Aurintricarboxylic Acid | 4431-00-9 | sc-3525 sc-3525A sc-3525B sc-3525C | 100 mg 1 g 5 g 10 g | $20.00 $32.00 $48.00 $94.00 | 13 | |
Aurintricarboxylic acid is known to inhibit nucleic acid enzymes and, by extension, can inhibit ATPases like DNAH5. It can bind to ATP-binding sites, thus potentially hindering DNAH5's ATPase activity which is crucial for its function in dynein arm assembly and ciliary beat. | ||||||
Sodium metavanadate | 13718-26-8 | sc-251034 sc-251034A | 5 g 25 g | $32.00 $84.00 | 3 | |
Vanadate is a transition state analog for phosphate groups and can inhibit ATPases. It may bind to DNAH5's ATPase domain, thereby blocking its ATP hydrolysis-driven motor activity, which is essential for ciliary movement. | ||||||
Emodin | 518-82-1 | sc-202601 sc-202601A sc-202601B | 50 mg 250 mg 15 g | $105.00 $214.00 $6255.00 | 2 | |
Emodin can inhibit protein kinase activity, which may affect the phosphorylation state of DNAH5. Proper phosphorylation is necessary for DNAH5's function, and inhibition of kinases that target DNAH5 can reduce its activity. | ||||||
Oligomycin | 1404-19-9 | sc-203342 sc-203342C | 10 mg 1 g | $149.00 $12495.00 | 18 | |
Oligomycin inhibits ATP synthase, and while not directly inhibiting DNAH5, by reducing cellular ATP levels, it can indirectly inhibit DNAH5's ATP-dependent motor functions. | ||||||
Sodium azide | 26628-22-8 | sc-208393 sc-208393B sc-208393C sc-208393D sc-208393A | 25 g 250 g 1 kg 2.5 kg 100 g | $43.00 $155.00 $393.00 $862.00 $90.00 | 8 | |
Sodium azide inhibits cytochrome c oxidase in the electron transport chain, thereby depleting cellular ATP levels and indirectly inhibiting DNAH5's ATP-dependent motor functions. | ||||||
Ruthenium red | 11103-72-3 | sc-202328 sc-202328A | 500 mg 1 g | $188.00 $250.00 | 13 | |
Ruthenium Red is an inhibitor of calcium channels. Given that calcium signaling can be involved in the regulation of ciliary function, this compound could indirectly inhibit DNAH5 by altering the calcium-dependent regulatory mechanisms. | ||||||
Alsterpaullone | 237430-03-4 | sc-202453 sc-202453A | 1 mg 5 mg | $68.00 $312.00 | 2 | |
Alsterpaullone is a cyclin-dependent kinase inhibitor which may affect the cell cycle and consequently the assembly of cilia, thus potentially inhibiting DNAH5 which is essential for ciliary movement. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin disrupts calcium stores in the endoplasmic reticulum and can perturb calcium signaling. This disturbance can indirectly inhibit DNAH5 by affecting its regulation involving calcium-dependent processes. | ||||||
Monastrol | 254753-54-3 | sc-202710 sc-202710A | 1 mg 5 mg | $120.00 $233.00 | 10 | |
Monastrol is a known kinesin Eg5 inhibitor. Although not a direct inhibitor of DNAH5, it affects microtubule dynamics. Since DNAH5 is associated with dynein arms and microtubules in cilia, changes in microtubule dynamics can indirectly inhibit its function. | ||||||
Mitoxantrone | 65271-80-9 | sc-207888 | 100 mg | $285.00 | 8 | |
Mitoxantrone intercalates into DNA and inhibits topoisomerase II, leading to disrupted DNA replication and transcription. This can indirectly inhibit DNAH5 by affecting the expression and assembly of ciliary components that are dependent on gene transcription. | ||||||