DIS3L2 Activators
DIS3L2 Activators encompass a variety of chemical compounds that indirectly boost the exonuclease's capacity to degrade RNA substrates. The nucleotide-derived molecule Adenosine triphosphate (ATP) is fundamental for DIS3L2's function, as it provides the energy required for the hydrolysis of RNA strands. Essential ions like Magnesium chloride (MgCl2) serve as crucial co-factors, ensuring the RNA substrate and the catalytic sites are properly aligned for optimal enzymatic action, while Potassium chloride (KCl) modulates the RNA structure, thus aiding DIS3L2's access and processing of RNA. The addition of Ammonium sulfate, known for its protein stabilization properties, could reinforce DIS3L2's structural integrity, facilitating sustained RNA turnover. Similarly, Spermidine assists in RNA stabilization, potentially augmenting the rate at which DIS3L2 can engage and degrade RNA molecules.
Further enhancing DIS3L2's functional activity are Zinc sulfate and Sodium orthovanadate; the former could heighten DIS3L2's RNA-binding affinity, while the latter may keep DIS3L2 in a phosphorylated, active state by inhibiting phosphatases. Glycerol and low concentrations of Urea are included to maintain the enzyme's structure under varied laboratory conditions, ensuring consistent activity. Tris(hydroxymethyl)aminomethane (Tris) is utilized to maintain a stable pH, which is paramount for DIS3L2's nuclease mechanism to function correctly. The role of Dithiothreitol (DTT) is to maintain the reduction of disulfide bonds within DIS3L2, preserving its active conformation, and Ethylene glycol tetraacetic acid (EGTA) enhances DIS3L2 activity indirectly by chelating potentially inhibitory cations such as Ca2+, ensuring that such ions do not adversely affect the exonuclease's activity. Collectively, these compounds support the biochemical machinery that enables DIS3L2 to effectively execute its role in RNA processing and degradation, thereby upholding the critical balance of RNA within the cell.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $73.00 $238.00 $717.00 $2522.00 $21420.00 | 53 | |
Actinomycin D binds to DNA and inhibits RNA polymerase, which leads to the blockage of RNA synthesis. This compound indirectly increases the need for DIS3L2 activity as the cell attempts to degrade the excess RNA that cannot be properly processed. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG132 is a proteasome inhibitor that causes the accumulation of polyubiquitinated proteins, which can lead to cellular stress and potentially increase the cellular demand for RNA turnover, thereby enhancing the activity of DIS3L2 in the RNA degradation pathway. | ||||||
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $150.00 $388.00 | 113 | |
Staurosporine is a protein kinase inhibitor that can induce cellular stress responses, possibly leading to an increased requirement for RNA turnover and upregulation of DIS3L2's role in RNA degradation. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
5-Azacytidine is a cytidine analog that gets incorporated into RNA and DNA, causing errors in the RNA and potentially triggering increased degradation of faulty RNA transcripts, which could enhance the activity of DIS3L2. | ||||||
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $68.00 | 2 | |
Chloroquine is known to inhibit lysosomal enzyme activities, which can cause cellular stress and potential changes in RNA turnover rates, possibly leading to an increased activity of DIS3L2 in RNA degradation pathways. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $169.00 $299.00 | 66 | |
Tunicamycin inhibits N-linked glycosylation, inducing ER stress, and as a result, may enhance the RNA degradation activity of DIS3L2 as part of the unfolded protein response. | ||||||
Mithramycin A | 18378-89-7 | sc-200909 | 1 mg | $54.00 | 6 | |
Mithramycin A binds to DNA and affects transcription processes. By changing RNA synthesis dynamics, it could lead to altered RNA turnover rates, potentially enhancing the activity of DIS3L2. | ||||||
Hydroxyurea | 127-07-1 | sc-29061 sc-29061A | 5 g 25 g | $76.00 $255.00 | 18 | |
Hydroxyurea inhibits ribonucleotide reductase, leading to reduced levels of deoxyribonucleotides, which can cause DNA damage and replication stress. This might upregulate RNA turnover mechanisms involving DIS3L2. | ||||||
17-AAG | 75747-14-7 | sc-200641 sc-200641A | 1 mg 5 mg | $66.00 $153.00 | 16 | |
17-AAG is an Hsp90 inhibitor that can destabilize various client proteins, leading to cellular stress and potentially increasing the role of DIS3L2 in degrading aberrant RNA molecules. | ||||||
Torin 1 | 1222998-36-8 | sc-396760 | 10 mg | $240.00 | 7 | |
Torin 1 is an mTOR inhibitor that can induce autophagy and cellular stress, potentially increasing the demand for RNA turnover and enhancing the activity of DIS3L2 in RNA degradation pathways. | ||||||