CROP Activators
CROP Activators are a select group of chemical compounds that enhance the functional activity of CROP through distinct and specific cellular signaling pathways. Forskolin, for instance, increases intracellular cAMP which activates PKA; this kinase may phosphorylate proteins in the pathway where CROP operates, resulting in the enhancement of CROP function. Similarly, PMA mimics DAG and robustly activates PKC, whichcould potentially phosphorylate CROP or initiate a cascade that includes CROP activation. Isoproterenol, by activating beta-adrenergic receptors, leads to a rise in cAMP and subsequent PKA activation, which again might result in the phosphorylation and activation of CROP. Ionomycin and A23187, both acting as calcium ionophores, elevate intracellular calcium levels, thereby potentially activating calcium-dependent kinases that could phosphorylate CROP if it is part of calcium signaling pathways. Sphingosine-1-phosphate engages with S1P receptors activating the PI3K/Akt pathway, which could lead to CROP activation if CROP is downstream of Akt or modulated by Akt.
Furthermore, Epigallocatechin gallate (EGCG) and LY294002, through inhibition of kinases and PI3K respectively, might upregulate alternative signaling pathways that activate CROP as a compensatory mechanism. Dibutyryl cyclic AMP (db-cAMP) bypasses receptor-mediated cAMP production and directly activates cAMP-dependent pathways, leading to a direct enhancement of CROP activity if CROP is responsive to such signals. The action of Anisomycin, as a protein synthesis inhibitor, can activate stress response pathways that may include CROP activation. Staurosporine's broad-spectrum kinase inhibition might relieve negative regulation on CROP, leading to its indirect activation. Lastly, U0126 inhibits MEK, which potentially causes the activation of alternative pathways that could enhance CROP activity, assuming CROP functions within those alternative pathways. Collectively, these chemicals employ a variety of intracellular signaling modifications to enhance the activity of CROP without directly increasing its expression or requiring direct ligand binding to CROP itself.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly stimulates adenylyl cyclase, leading to an increase in cyclic AMP (cAMP) levels. Elevated cAMP activates protein kinase A (PKA), which can then phosphorylate specific substrates leading to the activation of CROP, assuming CROP is a cAMP-responsive protein. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA acts as a diacylglycerol (DAG) analog and a potent activator of protein kinase C (PKC). Activation of PKC can initiate a signaling cascade that ultimately leads to the activation of CROP if CROP is a substrate for PKC or involved in PKC-mediated signaling pathways. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $27.00 $37.00 | 5 | |
Isoproterenol is a synthetic catecholamine and beta-adrenergic agonist. It activates beta-adrenergic receptors, leading to increased cAMP and activation of PKA. PKA can then phosphorylate and activate CROP if it is regulated by beta-adrenergic signaling pathways. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate calmodulin-dependent protein kinases (CaMK). If CROP is activated by CaMK or is part of calcium-dependent signaling pathways, ionomycin would enhance its activity. | ||||||
D-erythro-Sphingosine-1-phosphate | 26993-30-6 | sc-201383 sc-201383D sc-201383A sc-201383B sc-201383C | 1 mg 2 mg 5 mg 10 mg 25 mg | $162.00 $316.00 $559.00 $889.00 $1693.00 | 7 | |
Sphingosine-1-phosphate (S1P) is a lipid signaling molecule that activates S1P receptors and can lead to downstream signaling involving PI3K/Akt pathway. If CROP functions downstream of PI3K/Akt or is modulated by Akt phosphorylation, S1P would have an enhancing effect on CROP activity. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG is a polyphenol found in green tea that can inhibit certain protein kinases. By inhibiting competitive signaling pathways, EGCG might enhance signaling pathways that lead to CROP activation if such pathways are negatively regulated by the kinases EGCG inhibits. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is a PI3K inhibitor, which paradoxically can lead to the activation of alternative pathways that might enhance CROP activity, assuming CROP is part of a compensatory signaling mechanism when PI3K signaling is inhibited. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is another calcium ionophore, which increases intracellular calcium and activates calcium-dependent proteins and pathways. If CROP is part of these pathways, A23187 can enhance CROP activity by increasing the intracellular calcium concentration. | ||||||