Calcium Indicators

BAPTA/AM is a potent calcium chelator that selectively binds calcium ions, effectively modulating intracellular calcium levels. Its unique acetoxymethyl ester form facilitates cell permeability, allowing for rapid intracellular release upon hydrolysis. This compound exhibits distinct binding kinetics, enabling it to compete with calcium for binding sites, thereby influencing calcium-dependent signaling pathways. Its ability to form stable complexes with calcium ions alters cellular responses, providing insights into calcium homeostasis and signaling dynamics.

Coelenterazine is a bioluminescent compound that exhibits unique interactions with calcium ions, acting as a luminescent probe. Its structure allows for efficient energy transfer upon calcium binding, leading to the emission of light. This property is harnessed in various biochemical assays, where the kinetics of luminescence can provide insights into calcium fluctuations. The compound's sensitivity to calcium concentrations enables real-time monitoring of cellular processes, enhancing our understanding of calcium signaling dynamics.

CalciFluor™ Fluo-8, AM is a highly selective calcium indicator that exhibits remarkable fluorescence properties upon calcium ion binding. Its unique design facilitates rapid diffusion across cellular membranes, allowing for immediate intracellular calcium detection. The compound undergoes a significant conformational change upon calcium binding, resulting in enhanced fluorescence intensity. This dynamic response enables precise tracking of calcium signaling events, providing insights into cellular calcium homeostasis and signaling pathways.

Hydroxy naphthol blue disodium salt is a versatile chelating agent that exhibits a distinct affinity for calcium ions, forming stable complexes that alter its optical properties. Upon binding, it undergoes a notable colorimetric shift, which can be quantitatively measured. This interaction is characterized by specific coordination chemistry, where the hydroxyl and naphthol groups facilitate strong molecular interactions, enhancing its sensitivity to calcium fluctuations in various environments.

Calcein is a fluorescent dye that selectively binds to calcium ions, exhibiting a significant increase in fluorescence upon complexation. This property arises from its unique molecular structure, which allows for effective chelation through carboxylate groups. The resulting calcium-calcein complex demonstrates enhanced stability and distinct photophysical behavior, making it particularly responsive to changes in calcium concentration. This dynamic interaction is crucial for studying calcium dynamics in various biological systems.

FURA 2/AM is a cell-permeable fluorescent probe that selectively interacts with calcium ions, exhibiting a notable shift in fluorescence upon binding. Its unique design incorporates a dual-excitation wavelength mechanism, allowing for ratiometric measurements of calcium levels. This property enhances sensitivity to fluctuations in intracellular calcium concentrations. The probe's esterified form facilitates cellular uptake, where it is hydrolyzed to release the active FURA 2, enabling precise monitoring of calcium signaling pathways.

FURA-PE3/AM is a sophisticated fluorescent indicator that selectively binds calcium ions, resulting in a significant change in fluorescence intensity. Its unique structure allows for enhanced sensitivity to calcium fluctuations, with a rapid response time that facilitates real-time monitoring. The probe's lipophilic ester form ensures efficient cellular entry, where it is converted to the active FURA-PE3, enabling detailed analysis of calcium dynamics and cellular signaling mechanisms.

BAPTA tetrapotassium salt is a highly effective chelator of calcium ions, exhibiting a strong affinity that enables precise modulation of calcium levels in various environments. Its unique structure allows for rapid binding and release of calcium, influencing cellular signaling pathways. The compound's ability to form stable complexes with calcium enhances its role in studying calcium-dependent processes, making it a critical tool for investigating intracellular calcium homeostasis and dynamics.

FLUO 3/AM is a fluorescent calcium indicator that exhibits remarkable sensitivity to calcium ion fluctuations. Its unique esterified structure facilitates cellular permeability, allowing for efficient intracellular calcium monitoring. Upon binding to calcium, FLUO 3/AM undergoes a significant increase in fluorescence intensity, enabling real-time visualization of calcium dynamics. This property makes it an essential tool for exploring calcium-mediated signaling pathways and cellular responses.

CalciFluor™ Fluo-8L, AM is a highly sensitive fluorescent probe designed for detecting calcium ions within live cells. Its innovative design features a lipophilic ester that enhances membrane permeability, ensuring effective intracellular delivery. Upon calcium binding, the probe exhibits a pronounced fluorescence enhancement, allowing for precise tracking of calcium transients. This dynamic response aids in elucidating calcium's role in various cellular processes and signaling mechanisms.