APC7 Activators
APC7 Activators are a specialized group of chemical entities tailored to enhance the activity of Anaphase-Promoting Complex subunit 7 (APC7), a core component of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is crucial for cell cycle regulation, particularly in the transition from metaphase to anaphase during mitosis. These activators function by stabilizing the assembly of the APC/C complex or by facilitating the post-translational modifications required for the full activation of APC7. A distinctive feature of APC7 activators is their ability to bind to specific regulatory sites on APC7, enhancing its affinity for other subunits within the APC/C complex and thus promoting the ubiquitination activity that is central to cell cycle progression. Some activators may interact with the APC/C activator proteins, such as CDC20 or CDH1, potentiating their ability to stimulate the ubiquitin ligase activity of APC/C, which in turn leads to the targeted degradation of cell cycle regulators like securin and cyclins. This degradation is a pivotal step for chromatid separation and exit from mitosis.
The molecular intricacies of APC7 activators involve intricate interactions with the protein's conformational dynamics, often through allosteric effects that propagate structural changes to the active site of APC/C. These activators are characterized by their selective interactions with either the APC7 subunit itself or associated regulatory proteins that are part of the ubiquitin-proteasome pathway. By enhancing the ubiquitin ligase activity of APC/C, APC7 activators indirectly contribute to the temporal regulation of the cell cycle. Their actions ensure the fidelity of chromosomal segregation by precisely timing the ubiquitination and subsequent proteasomal degradation of key cell cycle proteins. This meticulous control by APC7 activators underscores their significance in maintaining cellular homeostasis and the orderly progression of the cell cycle. The specificity and selective activity of these chemical compounds make them pivotal in the context of cell cycle research, providing insights into the regulation of mitosis and influence cellular proliferation through the modulation of APC7.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin activates adenylate cyclase, leading to an increase in cAMP levels. Elevated cAMP activates PKA, which can phosphorylate various substrates, including those involved in the regulation of the cell cycle. Since APC7 is a component of the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that regulates the cell cycle, PKA-mediated phosphorylation events could enhance the ubiquitin ligase activity of the APC/C, thus indirectly enhancing the functional activity of APC7. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $60.00 $265.00 $1000.00 | 163 | |
MG132 is a proteasome inhibitor that prevents the degradation of ubiquitinated proteins. By inhibiting the proteasome, MG132 can lead to the accumulation of cell cycle regulators that are normally targeted for degradation by the APC/C, indirectly increasing the demand for APC7 activity as part of the feedback loop to enhance the degradation process once the inhibitor is removed. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is a potent inhibitor of protein phosphatases 1 and 2A. The inhibition of these phosphatases leads to increased phosphorylation levels of various proteins, including those involved in the cell cycle. This could indirectly enhance APC7 activity by affecting the substrate recognition or timing of ubiquitination by the APC/C. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid is a selective inhibitor of protein phosphatase 2A (PP2A). Inhibition of PP2A can lead to altered phosphorylation states of APC/C subunits or its substrates, potentially enhancing the activity of APC7 by affecting the timing of the cell cycle events that APC/C regulates. | ||||||
S-Trityl-L-cysteine | 2799-07-7 | sc-202799 sc-202799A | 1 g 5 g | $32.00 $66.00 | 6 | |
S-Trityl-L-cysteine is a selective inhibitor of the mitotic kinesin Eg5. Inhibition of Eg5 leads to mitotic arrest, similarly to paclitaxel, which could increase the functional demand on the APC/C, thus indirectly enhancing APC7 activity. | ||||||
ZM-447439 | 331771-20-1 | sc-200696 sc-200696A | 1 mg 10 mg | $153.00 $356.00 | 15 | |
ZM447439 is an Aurora kinase inhibitor. Aurora kinases are involved in the regulation of mitosis, and their inhibition can lead to mitotic arrest. Mitotic arrest would increase the requirement for APC/C function, potentially enhancing the activity of APC7. | ||||||
Roscovitine | 186692-46-6 | sc-24002 sc-24002A | 1 mg 5 mg | $94.00 $265.00 | 42 | |
Roscovitine is a cyclin-dependent kinase (CDK) inhibitor. CDKs are key regulators of the cell cycle, and their inhibition can result in cell cycle arrest. This arrest could indirectly enhance APC7 activity as part of the APC/C response to restore cell cycle progression. | ||||||
BI 2536 | 755038-02-9 | sc-364431 sc-364431A | 5 mg 50 mg | $151.00 $525.00 | 8 | |
BI 2536 is a Plk1 inhibitor. Polo-like kinase 1 (Plk1) is essential for mitosis, and its inhibition can lead to mitotic arrest. This could result in an increased functional demand on APC/C, potentially enhancing the activity of APC7. | ||||||
Monastrol | 254753-54-3 | sc-202710 sc-202710A | 1 mg 5 mg | $120.00 $233.00 | 10 | |
Monastrol is a selective inhibitor of the mitotic kinesin Eg5. By inhibiting Eg5, monastrol induces mitotic arrest, which could lead to an increased requirement for APC/C activity and indirectly enhance APC7 activity. | ||||||