ANKT Activators
Microtubule dynamics are at the heart of spindle assembly and function during cell division, and proteins like NUSAP1 are pivotal for their proper organization and stability. Chemical activators that influence these dynamics, even indirectly, modulate the activity of spindle assembly proteins such as NUSAP1. For instance, compounds like Epothilone B and Laulimalide stabilize microtubules by promoting polymerization, thereby increasing the demand for NUSAP1 activity. The intricate balance of microtubule assembly and disassembly is crucial during mitosis, and agents that affect this balance can lead to compensatory cellular responses that upregulate proteins involved in these processes. Stabilizing agents generally enhance the persistence of microtubules, increasing the stability of the mitotic spindle, which can indirectly necessitate the heightened activity of NUSAP1 to manage the augmented microtubule networks.
On the other hand, agents that initially disrupt microtubule dynamics might lead to an adaptive increase in NUSAP1 activity as part of the cellular attempt to salvage and stabilize the spindle apparatus. In this light, these compounds can be viewed as indirect activators, as their perturbation of the microtubule equilibrium drives cells to enhance the expression or activity of spindle-associated proteins. Similarly, taxanes like Docetaxel, which are well-known microtubule-stabilizing agents, contribute to a robust spindle structure that might indirectly boost the role of NUSAP1 in maintaining the integrity of spindle microtubules during the pressures of cell division. The physiological responses to these compounds suggest an angle where the modulation of spindle dynamics can lead to mitotic arrest and cell death in rapidly dividing tumor cells. However, their impact on NUSAP1 activity and the broader implications for cell cycle regulation highlight the complex interplay between drug-induced microtubule dynamics and spindle assembly protein function.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Diethylstilbestrol | 56-53-1 | sc-204720 sc-204720A sc-204720B sc-204720C sc-204720D | 1 g 5 g 25 g 50 g 100 g | $70.00 $281.00 $536.00 $1076.00 $2142.00 | 3 | |
Diethylstilbestrol interacts with estrogen receptors, leading to the transcription of genes involved in mitotic spindle formation. This can enhance NUSAP1 activity indirectly by increasing the cellular demand for spindle assembly proteins, thus promoting NUSAP1’s role in organizing spindle microtubules. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a potent activator of protein phosphatases PP1 and PP2A. The activation leads to dephosphorylation of numerous cellular proteins, potentially enhancing NUSAP1 function indirectly by affecting its phosphorylation state and promoting its role in spindle assembly. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $160.00 $750.00 | 59 | |
Similar to okadaic acid, Calyculin A is an inhibitor of protein phosphatases but can indirectly increase NUSAP1 activity by altering the phosphorylation landscape, possibly affecting proteins that regulate NUSAP1's association with spindle microtubules. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin acts as a protein synthesis inhibitor; however, at specific concentration levels, it can induce stress responses that lead to the upregulation of proteins involved in cell division, indirectly enhancing NUSAP1's role in spindle microtubule organization. | ||||||
Methotrexate | 59-05-2 | sc-3507 sc-3507A | 100 mg 500 mg | $92.00 $209.00 | 33 | |
Methotrexate is known for its antifolate activity, but its ability to induce a G2/M cell cycle arrest can indirectly result in the upregulation of NUSAP1, as cells enhance the expression of mitotic proteins to prepare for cell division. | ||||||
Griseofulvin | 126-07-8 | sc-202171A sc-202171 sc-202171B | 5 mg 25 mg 100 mg | $83.00 $216.00 $586.00 | 4 | |
Griseofulvin interferes with microtubule function by binding to tubulin, which can indirectly stimulate cellular mechanisms to enhance the role of NUSAP1 in spindle assembly and stability to counteract the disruption. | ||||||
Docetaxel | 114977-28-5 | sc-201436 sc-201436A sc-201436B | 5 mg 25 mg 250 mg | $85.00 $325.00 $1072.00 | 16 | |
Docetaxel is a microtubule-stabilizing agent that can indirectly increase NUSAP1 activity by promoting spindle microtubule assembly, thus potentially enhancing the requirement for NUSAP1 in maintaining spindle structure. | ||||||
Laulimalide | 115268-43-4 | sc-507261 | 100 µg | $200.00 | ||
Laulimalide acts to stabilize microtubules by binding to a site on tubulin different from that of paclitaxel, promoting polymerization. This stabilization could indirectly increase the functional demand for NUSAP1 in organizing the spindle microtubules. | ||||||
Parthenolide | 20554-84-1 | sc-3523 sc-3523A | 50 mg 250 mg | $79.00 $300.00 | 32 | |
Parthenolide has been shown to affect the stability of microtubules indirectly. While known for other cellular effects, it may enhance microtubule dynamics at certain concentrations, which could lead to an increase in NUSAP1 activity to support spindle formation. | ||||||