ZRSR1 CRISPR/Cas9 KO Plasmid (m): sc-423570
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZRSR1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZRSR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZRSR1 CRISPR/Cas9 KO Plasmid (m) | sc-423570 | 20 µg | $397.00 |
Overview
Zrsr1 encodes ZRSR1, a spliceosome-associated factor implicated in recognition and processing of U12-type introns during pre-mRNA splicing. By influencing minor spliceosome activity, ZRSR1 can affect transcript maturation, isoform balance, and downstream gene expression programs linked to cell cycle control and differentiation. In mouse, Zrsr1 is also notable in the context of genomic imprinting and parent-of-origin effects, providing a framework to study allele-specific regulation of RNA processing. Dysregulation of minor intron splicing has been connected to developmental abnormalities and hematopoietic dysfunction in multiple model systems, making Zrsr1 relevant for mechanistic studies of splicing-associated disease biology.
ZRSR1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zrsr1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zrsr1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zrsr1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZRSR1 protein expression.
This CRISPR knockout system enables efficient generation of Zrsr1-deficient cell models for investigation of ZRSR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zrsr1 exon(s) critical for ZRSR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zrsr1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZRSR1 CRISPR/Cas9 KO Plasmid (m) and ZRSR1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zrsr1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZRSR1 HDR Plasmid (m) and ZRSR1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zrsr1 homology arms to support homology-directed repair at defined Zrsr1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.