Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZBTB4 CRISPR/Cas9 KO Plasmid (h): sc-404975
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZBTB4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZBTB4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZBTB4 Antibody (A-7): sc-514883
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZBTB4 CRISPR/Cas9 KO Plasmid (h) | sc-404975 | 20 µg | $397.00 |
Overview
ZBTB4 (zinc finger and BTB domain-containing protein 4) is a sequence-specific transcriptional regulator that binds methylated CpG DNA and contributes to epigenetic control of gene expression. Through its BTB/POZ domain and zinc finger motifs, ZBTB4 participates in chromatin-associated repression programs that influence cell-cycle progression, DNA damage responses, and differentiation-associated transcriptional states. Altered ZBTB4 expression or activity has been linked to dysregulated proliferation and invasive phenotypes across multiple cancer contexts, consistent with a role in maintaining transcriptional homeostasis. As a methylation-sensitive factor, ZBTB4 is also relevant for studies connecting DNA methylation patterns to downstream transcriptional outputs and genome stability.
ZBTB4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZBTB4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZBTB4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZBTB4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZBTB4 protein expression.
This CRISPR knockout system enables efficient generation of ZBTB4-deficient cell models for investigation of ZBTB4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZBTB4 exon(s) critical for ZBTB4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZBTB4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZBTB4 CRISPR/Cas9 KO Plasmid (h) and ZBTB4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZBTB4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZBTB4 HDR Plasmid (h) and ZBTB4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZBTB4 homology arms to support homology-directed repair at defined ZBTB4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.