TEST CONDITIONS
HPLC Conditions: Column: Beckman Ultrasphere 5 µm, 250 x 4.6 mm, 150 ÅGuard Column: Supel Guard LC-18-DB, 2 cmRetention Time: 6.63 minSample preparation: 2 mg/1 ml 100% Acetonitrile/WaterInjection volume: 5 µlMethod /Detection at, nm Gradient 30 - 80% B A = 99.9% Water, 0.1% TFA Boc / 210 B = 95% Acetonitrile, 4.9% Water, 0.1% TFA, 30 min, flow rate = 1.0 ml / minExp. Date: 8/30/2023
