Date published: 2026-10-19
访问圣克鲁斯动物健康

1-800-457-3801

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
WT1 Antibody (H-1) - Immunohistochemistry - Image 351538
WT1 抗体 (H-1): sc-393498. 福尔马林固定, 石蜡包埋的人睾丸组织的免疫过氧化物酶染色, 显示曲细精管和雷迪格细胞的细胞质染色.
WT1 抗体 (H-1): sc-393498. 福尔马林固定, 石蜡包埋的人睾丸组织的免疫过氧化物酶染色, 显示曲细精管和雷迪格细胞的细胞质染色.

WT1 抗体 (H-1): sc-393498

3.1(14)
寫評論提問
说明书
  • WT1抗体(H-1)是小鼠单克隆IgM κ, 在23篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的WT1的内部区域内的氨基酸123-164之间
  • 推荐用于 mouse, rat 和 human 来源的WT1 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： 和 bovine and porcine
  • 目前，我们还没有完成WT1 抗体 (H-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。
Gene Editing Promo Banner

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

WT1抗体（H-1）是一种IgM κ小鼠单克隆WT1抗体，可通过WB、IP、IF、IHC(P)和ELISA检测到来源于小鼠、大鼠和人类的WT1。WT1抗体（H-1）提供非偶联的抗体形式。Wilms瘤（WT）是一种影响肾脏的胚胎恶性肿瘤，影响比例为万分之一的婴儿，并且像视网膜母细胞瘤一样，既有散发形式也有遗传形式。Wilms瘤基因座已被定位在11p13染色体上，作为一种抑癌基因，编码一种具有四个锌指和一个富含谷氨酰胺-脯氨酸的氨基末端的DNA结合蛋白。Wilms瘤蛋白（WT1）与DNA序列GCGGGGGCG结合，这是早期生长反应（Egr）家族的Zn2+指转录激活因子常见的识别元件。然而，与Egr转录因子相反，WT1在与合成启动子结构的瞬时转染测定中表现为转录抑制因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

WT1 抗体 (H-1) 参考文献:

  1. 肾母细胞瘤的发展：从WT1和microRNA到动物模型。  |  Tian, F., et al. 2014. Biochim Biophys Acta. 1846: 180-7. PMID: 25018051
  2. Wilms肿瘤1（WT1）蛋白：小儿肿瘤的诊断用途。  |  Salvatorelli, L., et al. 2015. Acta Histochem. 117: 367-78. PMID: 25881478
  3. Wilms肿瘤-1（WT1）rs16754多态性是急性髓细胞白血病（AML）的预后因素：荟萃分析。  |  Long, J., et al. 2016. Oncotarget. 7: 32079-87. PMID: 26992216
  4. 急性髓细胞白血病发病机制中的肾母细胞瘤1突变。  |  Rampal, R. and Figueroa, ME. 2016. Haematologica. 101: 672-9. PMID: 27252512
  5. 以 Wilms' tumor 1 (WT1) 为靶点的癌症疫苗可延长胰腺癌患者的生存期。  |  Koido, S., et al. 2016. Immunotherapy. 8: 1309-1320. PMID: 27993090
  6. 肾母细胞瘤1（WT1）在发育、稳态和疾病中的作用。  |  Hastie, ND. 2017. Development. 144: 2862-2872. PMID: 28811308
  7. Wilms肿瘤1基因在造血恶性肿瘤中的临床意义和未来方向。  |  Luo, P., et al. 2020. Leuk Lymphoma. 61: 2059-2067. PMID: 32401109
  8. Wilms' tumor 1（WT1）通过调节 E 角连蛋白和 ERK1/2 信号传导促进卵巢癌的进展。  |  Han, Y., et al. 2020. Cell Cycle. 19: 2662-2675. PMID: 32892698
  9. 威尔姆斯肿瘤1（WT1）：癌症疫苗。  |  Hein, KZ., et al. 2020. J Immunother Precis Oncol. 3: 165-171. PMID: 35665371
  10. Wilms肿瘤1（WT1）抗原在卡波西肉瘤中过度表达，并由KSHV vFLIP调节。  |  Morales, AE., et al. 2024. PLoS Pathog. 20: e1011881. PMID: 38190392

订购信息

产品名称产品编号规格价格数量收藏夹

WT1 抗体 (H-1)

sc-393498
200 µg/ml
$322.00

WT1 (H-1) 中和勝肽

sc-393498 P
100 µg/0.5 ml
$69.00

I would like to use the WT-1 (H-1) antibody to stain for podocytes in kidney organoids. Do you have any secondary antibody to recommend for fluorescence microscopy?

Asked by: ValeM
Thank you for your question. Here is a link to our secondary antibodies: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-05-21

Hi, can I ask for the antibody WT1 (H1) insert. Actually I'm looking for WT1 which detect carboxyl Terminus or C-Terminus. Is this is the correct one? to rule out DSRCT (Desmoplastic Small Round Cell Tumours). Can I have the sample for evaluation? TQ

Asked by: RUZA
Thank you for your question. This mouse monoclonal IgM antibody is specific for an epitope mapping between amino acids 123-164 within an internal region of WT1 of human origin. It does not specifically recognize the C-terminus. We do not currently offer any WT1 antibodies with C-terminal epitopes. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-03-03

I am using WT1 (H-1): sc-393498 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6.0) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-01-20
  • y_2026, m_10, d_17, h_10CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_393498, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 95ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by Courteney from Works with problemsIt works, but there is always a prominent second band. The second band is very close to the band for WT1 and is thicker and more intense than the WT1 band.
Date published: 2022-08-02
Rated 1 out of 5 by Fra_vet from Non funziona su tessuti FFPE di gattoHo ordinato un sample di questo anticorpo per marcare tessuti di gatto fissati in formalina e inclusi in paraffina. Purtroppo non ho riscontrato nessun segnale. Campioni usati: rene fetale con glomeruli immaturi, mesotelioma, nefroblastoma
Date published: 2021-12-28
Rated 1 out of 5 by Sugawara from 免疫組織化学でうまく機能しない精巣のパラフィン切片を使って免疫蛍光染色を行いましたが、 セルトリ細胞の核が全く染色されませんでした。
Date published: 2020-12-23
Rated 5 out of 5 by Sadek from Dr SadekAb work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 1 out of 5 by zhengxiaoyi from No IHC or IF staining at all.We acquire a sample and perform IHC and IF on 4um mouse kidney section with M.O.M. kit. None of them works. Podocytes does not have nuclear staining of WT-1, nor has cytoplasmic staining. It`s just completely negative. I really hope the santa Cruz can resume C-19 (sc-192) since it works perfectly on frozen sections with 4% PFA fixation of 20min.
Date published: 2018-08-23
Rated 1 out of 5 by Shan_2018 from WT1 antibody Sc-393498Purchased this antibody recently. Tried it on WB and IP-MS experiments. Didn't work in either. :((
Date published: 2018-05-07
Rated 1 out of 5 by qltang from 染的都是背景I applied a sample sack in Sep. and then I did IF in both testis and heart. Results showed that the nonspecific staining as it stained in cytoplasm and myocardiocytes where supposed not be appear.
Date published: 2017-10-12
Rated 5 out of 5 by J JL from Good, BUTAntibody is perfect, but it maybe unsuitable to replace C-19, because my aim is detect EWS-WT1. The service of SCBT is very nice!
Date published: 2017-07-31
  • y_2026, m_10, d_18, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_zh_CN, sid_393498, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
WT1 抗体 (H-1) is rated 3.1 out of 5 by 14.
  • y_2026, m_10, d_18, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.48
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_zh_CN, sid_393498, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 96ms
  • REVIEWS, PRODUCT
1-800-457-3801
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打1-800-457-3801联系我们。
版权所有© 2007-2026 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。