Date published: 2025-9-7
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WASP Antibody (B-9) - Western Blotting - Image 354796
WASP 抗体 (B-9): sc-13139. 在 MOLT-4 (A), K-562 (B), TK-1 (C), WEHI-231 (D) 和 RBL-1 (E) 全细胞裂解液中 WASP 表达的 Western 印迹分析.
WASP 抗体 (B-9): sc-13139. 在 MOLT-4 (A), K-562 (B), TK-1 (C), WEHI-231 (D) 和 RBL-1 (E) 全细胞裂解液中 WASP 表达的 Western 印迹分析.

WASP 抗体 (B-9): sc-13139

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说明书
  • WASP 抗体 B-9 是小鼠单克隆 IgG2a κ，WASP抗体, 在72篇文献中引用，规格为200 µg/ml
  • 免疫human物种的WASP的氨基酸1-250
  • WASP 抗体 (B-9) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat, human 和mink 来源的 WASP and N-WASP
  • 抗WASP抗体（B-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取WASP (B-9): sc-13139的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 WASP 抗体 (B-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与WASP 抗体 (B-9) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

WASP抗体（B-9）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠、人类和貂的WASP蛋白。抗WASP抗体（B-9）是针对人源WASP的1-250个氨基酸产生的。WASP（B-9）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Wiskott-Aldrich综合征（WAS）是一种与X染色体短臂突变相关的遗传疾病，其特征是血小板减少、湿疹、免疫系统缺陷和淋巴增生性疾病风险增加等症状。WAS基因编码WASP，这是一种富含脯氨酸的蛋白质，在细胞骨架组织中起着至关重要的作用。WASP主要位于细胞质中，对调节肌动蛋白聚合至关重要，而肌动蛋白聚合对细胞运动、形状和分裂等细胞过程至关重要。WASP在细胞中的位置与Arp2/3复合物的相互作用有关，Arp2/3复合物有助于新的肌动蛋白丝的成核，从而影响细胞骨架的动态。WASP功能的破坏会导致严重的免疫学后果，这凸显了WASP在维持细胞结构和功能方面的重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

WASP 抗体 (B-9) 参考文献:

  1. Cdc42-互作蛋白4通过激活N-WASp促进乳腺癌细胞侵袭和形成侵袭嵴。  |  Pichot, CS., et al. 2010. Cancer Res. 70: 8347-56. PMID: 20940394
  2. N-WASP 通过调节 p38 MAPKs 信号通路促进宫颈癌细胞的侵袭和迁移。  |  Hou, J., et al. 2017. Am J Transl Res. 9: 403-415. PMID: 28337270
  3. Wiskott-Aldrich综合征蛋白（WASP）是T细胞淋巴瘤的肿瘤抑制因子。  |  Menotti, M., et al. 2019. Nat Med. 25: 130-140. PMID: 30510251
  4. WASp 在 T 细胞和 B 细胞中的作用。  |  Sun, X., et al. 2019. Cell Immunol. 341: 103919. PMID: 31047647
  5. 巨噬细胞线粒体裂变可提高治疗性抗体诱导的癌细胞吞噬能力，并因谷氨酰胺竞争而受损。  |  Li, J., et al. 2022. Nat Cancer. 3: 453-470. PMID: 35484420
  6. Wiskott Aldrich综合征蛋白（WASp）缺陷的Th1细胞在T细胞急性淋巴细胞白血病的发病机制中促进R环驱动的转录不足和转录耦合核苷酸切除修复因子（TC-NER）驱动的基因组不稳定性。  |  R, P., et al. 2024. Clin Immunol. 263: 110204. PMID: 38582251
  7. 肌动蛋白成核促进因子N-WASP影响α-突触核蛋白凝聚物和病理学。  |  Jackson, J., et al. 2024. Cell Death Dis. 15: 304. PMID: 38693139
  8. 维斯科特-阿尔德里奇综合征蛋白是 GTPase CDC42Hs 的一种新型效应物，与肌动蛋白聚合反应有关。  |  Symons, M., et al. 1996. Cell. 84: 723-34. PMID: 8625410
  9. 威斯科特-阿尔德里奇综合征血细胞缺陷。  |  Remold-O'Donnell, E., et al. 1996. Blood. 87: 2621-31. PMID: 8639877
  10. Wiskott-Aldrich 综合征蛋白与 GTPase Cdc42 的直接相互作用。  |  Kolluri, R., et al. 1996. Proc Natl Acad Sci U S A. 93: 5615-8. PMID: 8643625
  11. 研究威斯科特-阿尔德里奇综合征蛋白的表达。  |  Stewart, DM., et al. 1996. J Clin Invest. 97: 2627-34. PMID: 8647957
  12. 威斯科特-阿尔德里奇综合征：基因型与表型之间没有严格的相关性，但在 WASP 基因产物的氨基末端部分存在错义突变群。  |  Schindelhauer, D., et al. 1996. Hum Genet. 98: 68-76. PMID: 8682510

订购信息

产品名称产品编号规格价格数量收藏夹

WASP 抗体 (B-9)

sc-13139
200 µg/ml
$316.00

WASP (B-9): m-IgGκ BP-HRP 套装

sc-520603
200 µg Ab, 40 µg BP
$354.00

WASP 抗体 (B-9) AC

sc-13139 AC
500 µg/ml, 25% agarose
$416.00

WASP 抗体 (B-9) HRP

sc-13139 HRP
200 µg/ml
$316.00

WASP 抗体 (B-9) FITC

sc-13139 FITC
200 µg/ml
$330.00

WASP 抗体 (B-9) PE

sc-13139 PE
200 µg/ml
$343.00

WASP 抗体 (B-9) Alexa Fluor® 488

sc-13139 AF488
200 µg/ml
$357.00

WASP 抗体 (B-9) Alexa Fluor® 546

sc-13139 AF546
200 µg/ml
$357.00

WASP 抗体 (B-9) Alexa Fluor® 594

sc-13139 AF594
200 µg/ml
$357.00

WASP 抗体 (B-9) Alexa Fluor® 647

sc-13139 AF647
200 µg/ml
$357.00

WASP 抗体 (B-9) Alexa Fluor® 680

sc-13139 AF680
200 µg/ml
$357.00

WASP 抗体 (B-9) Alexa Fluor® 790

sc-13139 AF790
200 µg/ml
$357.00

We are using WASP Antibody (B-9) PE in routine testing. I need to put isotype control along with the test. Can you please help me in finding the one for the mentioned antibody?

Asked by: Rashmi
Thank you for your question. Here is a link to our normal mouse IgG2a conjugated to PE: https://www.scbt.com/p/normal-mouse-igg2a-pe This should be perfect as a control for you.
Answered by: Tech Service
Date published: 2020-01-15

We are using WASP Antibody (B-9) PE for the diagnosis of Wiskott Aldrich syndrome through flow cytometry. I need to put an experiment with isotype control. I am unable to find the one on the site. Can you please help me in finding?

Asked by: Rashmi
Thank you for your question. Here is a link to our isotype controls: https://www.scbt.com/browse/Control-IgGs-and-IgG-Conjugates/_/N-1j3ajah Please note that our products, including our WASP antibodies, are not recommended or warrantied for diagnostic use.
Answered by: Tech Service
Date published: 2020-01-14

Can the Antibody WASP (B-9) sc-13139 be used in flow cytometer for human blood analysis? And what is the difference with the antibody WASP D-1sc-5300?

Asked by: giusi85
Thanks for your question. WASP (B-9) is recommended for detection of WASP and N-WASP in human samples by FCM. The difference between the WASP (B-9) and WASP (D-1) is the isotype being mouse IgG2a κ and mouse IgG1 κ respectively. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can this antibody be used in IHC with paraffin-embedded samples?

Asked by: TinTin
Thank you for your question. Yes, this WASP antibody, sc-13139, has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 13
Date published: 2017-01-20

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, WASP (B-9), is currently available conjugated to agarose (sc-13139 AC), HRP (sc-13139 HRP), phycoerythrin (sc-13139 PE), fluorescein (sc-13139 FITC), Alexa Fluor® 488 (sc-13139 AF488) and Alexa Fluor® 647 (sc-13139 AF647).
Answered by: Technical Support 9
Date published: 2024-12-17
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Rated 5 out of 5 by from Excelent qualitywe highlight the good quality of the antibodies, which has allowed diagnostic guidance in patients in who are suspected deficiency of WAS and DOCK8 proteins.
Date published: 2019-11-26
Rated 4 out of 5 by from WASPI used this antibody for western blot and worked really good.
Date published: 2018-04-19
Rated 5 out of 5 by from Tested for IF: very good stainingTested at 2 ug/ml in PFA/tryton fixed/permeabilized primary cells. It works very well
Date published: 2017-10-05
Rated 5 out of 5 by from Recommend buyRecommend use Hela cell as positive control for WB detection, we tried dilution 1:500 and 1:800, strong signal at 66kda.60ug sample loading and use 10% SDS-PAGE.
Date published: 2017-09-16
Rated 5 out of 5 by from A variety of detection methods are obviousOur laboratory staff are very fond of using sc-13139 antibodies, we often use the detection of WB and IHC, the effect is very obvious.
Date published: 2017-02-05
Rated 5 out of 5 by from Works very wellIn the experiment, WASP antibody works very well, the analysis results of Western blot were very strong and clear.
Date published: 2017-02-04
Rated 5 out of 5 by from Outstanding productGreat western, strong signal; also nice IHC, IF and FCM data !!
Date published: 2017-01-26
Rated 5 out of 5 by from Great for WBWASP (B-9) works well for Western blot (WB). Strong band at 66 kDa in positive control K-562 Whole Cell Lysate: sc-2203.
Date published: 2017-01-18
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WASP 抗体 (B-9) is rated 4.8 out of 5 by 17.
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