VpreB1 CRISPR/Cas9 KO Plasmid (m): sc-423683
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VpreB1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VpreB1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VpreB1 CRISPR/Cas9 KO Plasmid (m) | sc-423683 | 20 µg | $397.00 |
Overview
Vpreb1 encodes VpreB1, a surrogate light chain component that pairs with λ5 (IGLL1) and the immunoglobulin μ heavy chain to form the pre–B cell receptor (pre-BCR) during early B lymphopoiesis in mouse. Pre-BCR signaling coordinates checkpoint progression from pro–B to pre–B stages by promoting proliferation, enforcing heavy-chain allelic exclusion, and initiating light-chain recombination through pathways that intersect with SRC family kinases, SYK/BTK signaling, and downstream PI3K–AKT and MAPK programs. Disruption of this axis perturbs B-cell developmental dynamics, alters repertoire formation, and can influence immune competence and tolerance mechanisms. Because pre-BCR function is tightly linked to developmental checkpoint control, Vpreb1 is commonly studied in models of aberrant B-cell maturation and immune dysregulation relevant to hematologic and autoimmune research.
VpreB1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vpreb1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vpreb1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vpreb1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VpreB1 protein expression.
This CRISPR knockout system enables efficient generation of Vpreb1-deficient cell models for investigation of VpreB1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Vpreb1 exon(s) critical for VpreB1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Vpreb1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VpreB1 CRISPR/Cas9 KO Plasmid (m) and VpreB1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vpreb1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VpreB1 HDR Plasmid (m) and VpreB1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vpreb1 homology arms to support homology-directed repair at defined Vpreb1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.