Date published: 2025-9-8

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VGAT 抗体 (F-2): sc-393373

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说明书
  • VGAT 抗体 F-2 是小鼠单克隆 IgM κ,VGAT抗体, 在8篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的VGAT的N-terminus附近的氨基酸71-98之间
  • 推荐用于 mouse, rat 和 human 来源的VGAT WB, IP, IF 和 ELISA检测
  • 关于如何获取VGAT (F-2): sc-393373免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是VGAT Antibody (F-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与VGAT Antibody (F-2) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

VGAT抗体(F-2)是一种IgM κ小鼠单克隆VGAT抗体(也被指定为VGAT抗体),可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的VGAT蛋白。VGAT抗体(F-2)可作为非偶联形式的抗VGAT抗体使用。突触传递涉及对含有特定神经递质的囊泡进行受控的胞吐作用。通常,神经递质在细胞质中合成,必须被运输到突触囊泡中才能释放。囊泡GABA转运体(VGAT)负责将抑制性神经递质γ-氨基丁酸(GABA)从神经元细胞质中加载到突触囊泡中,并且仅在含有GABA和/或甘氨酸的抑制性神经元的神经末梢中表达。在新皮质发育过程中,VGAT的表达几乎先于抑制性突触形成的成熟,这表明它可能有助于新皮质GABA能回路的发展。VGAT也可能在癫痫发生和自发性癫痫发作后的恢复机制中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VGAT 抗体 (F-2) 参考文献:

  1. 大鼠大脑皮层囊泡 GABA 转运体的产后发育  |  Minelli, A., et al. 2003. Neuroscience. 117: 337-46. PMID: 12614674
  2. 乙醇诱导新生大鼠大脑皮层切片培养物中的 GAD67 和 VGAT。  |  Zink, M. and Spanagel, R. 2005. Neuroreport. 16: 377-80. PMID: 15729141
  3. 小鼠囊泡抑制性氨基酸转运体基因:胚胎发育过程中的表达, 神经干细胞中核心启动子的分析以及对其交替剪接的重新考虑。  |  Oh, WJ., et al. 2005. Gene. 351: 39-49. PMID: 15826867
  4. 局灶性缺血后,大鼠脑内泡状 GABA 转运体的表达减少,而泡状谷氨酸, 乙酰胆碱和单胺转运体的表达则没有减少。  |  Vemuganti, R. 2005. Neurochem Int. 47: 136-42. PMID: 15936116
  5. 母体缺氧和硫酸镁负荷后成年后代海马 GABA 能系统的重塑:免疫组化研究。  |  Gerstein, M., et al. 2005. Exp Neurol. 196: 18-29. PMID: 16081066
  6. 大脑新皮层中表达 VGLUT1 的轴突末端的异质性。  |  Conti, F., et al. 2005. Arch Ital Biol. 143: 127-32. PMID: 16106993
  7. 人类垂体和生长激素腺瘤中的γ-氨基丁酸受体及合成和储存部位。  |  End, K., et al. 2005. Am J Clin Pathol. 124: 550-8. PMID: 16146821
  8. 突触素 I 和 II 的缺失会导致特定神经递质的囊泡转运减少。  |  Bogen, IL., et al. 2006. J Neurochem. 96: 1458-66. PMID: 16478532
  9. 光栓性脑梗塞后大鼠脑内囊泡 GABA 转运体的稳定表达。  |  Frahm, C., et al. 2006. Neuroscience. 140: 865-77. PMID: 16616431
  10. 囊泡 GABA 转运体 (VGAT) 表达减少的小鼠甘氨酸能突触传递受损,炎症性疼痛加剧。  |  Yamada, MH., et al. 2012. Mol Pharmacol. 81: 610-9. PMID: 22275517
  11. 激活腹侧被盖区囊泡GABA转运体(Vgat)神经元可减轻APP/PS1小鼠社交失败压力诱发的焦虑。  |  Yao, D., et al. 2023. Front Aging Neurosci. 15: 1142055. PMID: 37032820
  12. 神经髓鞘素 S+ 神经元中的囊泡 GABA 转运体的表达可维持行为昼夜节律。  |  Bussi, IL., et al. 2023. Proc Natl Acad Sci U S A. 120: e2314857120. PMID: 38019855

订购信息

产品名称产品编号规格价格数量收藏夹

VGAT 抗体 (F-2)

sc-393373
200 µg/ml
$316.00

VGAT (F-2): m-IgGκ BP-HRP 套装

sc-523920
200 µg Ab, 40 µg BP
$354.00

VGAT (F-2) 中和勝肽

sc-393373 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-393373 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ICC vGAT in striatal cortical rat co-cultureICC vGAT primary with AlexaFluor 488 in striato-cortical co-culture from dissociated mouse prep at E18.5.
Date published: 2017-09-18
Rated 5 out of 5 by from strong bandThis antibody been used to detect VGAT antibody in human Hela cell lysates, It worked very well in WB, 1:1000 diluted the antibody ,strong band.
Date published: 2017-09-01
Rated 5 out of 5 by from ExcellentGood product for Western Blot experiment in rat brain tissue, It works very well.
Date published: 2017-04-28
Rated 5 out of 5 by from Terrific antibody against VGAT!Excellent antibody for Western blot and IF. Nice strong bands seen in WB and clean IF staining. Great choice!
Date published: 2017-01-18
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-06
Rated 5 out of 5 by from Satisfactory Western Blot data of VGAT expressionSatisfactory Western Blot data of VGAT expression in non-transfected and human VGAT transfected 293T whole cell lysates and mouse brain and rat brain tissue extracts. -SCBT QC
Date published: 2014-08-25
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VGAT 抗体 (F-2) is rated 5.0 out of 5 by 6.
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