Date published: 2025-9-5

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VEGF 抗体 (C-1): sc-7269

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说明书
  • VEGF 抗体 C-1 是小鼠单克隆 IgG1 κ,VEGF抗体, 在1,270篇文献中引用,规格为200 µg/ml
  • 与human的1-140/氨基酸残基的VEGF
  • VEGF 抗体 (C-1) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 the 189, 165 and 121 amino acid splice variants of VEGF
  • 抗VEGF抗体(C-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VEGF (C-1): sc-7269免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 VEGF 抗体 (C-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与VEGF 抗体 (C-1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

VEGF抗体(C-1)是一种小鼠单克隆IgG1κ轻链抗体,可通过WB、IP、IF和IHCP检测小鼠、大鼠和人源VEGF蛋白。VEGF抗体(C-1)有非结合型和多种结合型,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合型。VEGF在血管生成(新血管形成过程)中起着至关重要的作用,而血管生成对于向组织输送营养和氧气至关重要,尤其是在发育和受伤时。VEGF与肿瘤进展有关,因为VEGF促进肿瘤供血血管的生长,从而促进肿瘤的生长和转移。VEGF以二聚体糖蛋白的形式存在,与血小板衍生生长因子(PDGF)具有结构同源性,并通过可变mRNA剪接产生多种同工型。在各种染色应用中,VEGF 单克隆抗体(C-1)显示细胞质定位,突出了 VEGF 在肿瘤细胞中的存在,而肿瘤细胞中 VEGF 通常过度表达。此外,原发性肿瘤中的 VEGF mRNA 表达和体外肿瘤细胞系中的 VEGF 产生强调了 VEGF 在癌症生物学中的重要性。值得注意的是,Flt 是 PDGF 受体家族的一员,已被确定为 VEGF 的高亲和力受体,进一步强调了 VEGF 在正常生理过程和病理条件下的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VEGF 抗体 (C-1) 参考文献:

  1. 血管内皮生长因子是人类胶质瘤体内潜在的肿瘤血管生成因子。  |  Plate, KH., et al. 1992. Nature. 359: 845-8. PMID: 1279432
  2. fms 样酪氨酸激酶,血管内皮生长因子的受体。  |  de Vries, C., et al. 1992. Science. 255: 989-91. PMID: 1312256
  3. 血管通透性因子(血管内皮生长因子)基因在正常组织, 巨噬细胞和肿瘤中的表达存在差异。  |  Berse, B., et al. 1992. Mol Biol Cell. 3: 211-20. PMID: 1550962
  4. 胚胎血管生成和内皮细胞分化过程中血管内皮生长因子的表达。  |  Breier, G., et al. 1992. Development. 114: 521-32. PMID: 1592003
  5. 血管内皮生长因子的人类基因。通过替代外显子剪接编码多种蛋白质形式。  |  Tischer, E., et al. 1991. J Biol Chem. 266: 11947-54. PMID: 1711045
  6. 血管内皮生长因子多肽家族。  |  Ferrara, N., et al. 1991. J Cell Biochem. 47: 211-8. PMID: 1791185
  7. 从大鼠胶质瘤衍生细胞系中纯化出一种糖蛋白血管内皮细胞有丝分裂原。  |  Conn, G., et al. 1990. Proc Natl Acad Sci U S A. 87: 1323-7. PMID: 2406719
  8. 在增生向肿瘤过渡期间诱导血管生成。  |  Folkman, J., et al. 1989. Nature. 339: 58-61. PMID: 2469964
  9. VEGF--中枢神经系统和周围神经系统中神经元发育和再生的刺激物。  |  Theis, V. and Theiss, C. 2018. Curr Protein Pept Sci. 19: 589-597. PMID: 29299985
  10. 血管内皮生长因子(VEGF)--正常和病理血管生成的关键因素。  |  Melincovici, CS., et al. 2018. Rom J Morphol Embryol. 59: 455-467. PMID: 30173249
  11. 靶向VEGF-神经丝蛋白相互作用:一种有前景的抗肿瘤策略。  |  Peng, K., et al. 2019. Drug Discov Today. 24: 656-664. PMID: 30315890
  12. 用于组织切片中血管内皮生长因子免疫组化定位的抗血管内皮生长因子(anti-VEGF)抗体的验证:血管内皮生长因子在人类子宫内膜中的表达。  |  Zhang, L., et al. 1998. J Pathol. 185: 402-8. PMID: 9828839

订购信息

产品名称产品编号规格价格数量收藏夹

VEGF 抗体 (C-1)

sc-7269
200 µg/ml
$316.00

VEGF (C-1): m-IgG Fc BP-HRP 套装

sc-526491
200 µg Ab; 10 µg BP
$354.00

VEGF (C-1): m-IgG1 BP-HRP 套装

sc-531864
200 µg Ab; 20 µg BP
$354.00

VEGF 抗体 (C-1) AC

sc-7269 AC
500 µg/ml, 25% agarose
$416.00

VEGF 抗体 (C-1) HRP

sc-7269 HRP
200 µg/ml
$316.00

VEGF 抗体 (C-1) FITC

sc-7269 FITC
200 µg/ml
$330.00

VEGF 抗体 (C-1) PE

sc-7269 PE
200 µg/ml
$343.00

VEGF 抗体 (C-1) Alexa Fluor® 488

sc-7269 AF488
200 µg/ml
$357.00

VEGF 抗体 (C-1) Alexa Fluor® 546

sc-7269 AF546
200 µg/ml
$357.00

VEGF 抗体 (C-1) Alexa Fluor® 594

sc-7269 AF594
200 µg/ml
$357.00

VEGF 抗体 (C-1) Alexa Fluor® 647

sc-7269 AF647
200 µg/ml
$357.00

VEGF 抗体 (C-1) Alexa Fluor® 680

sc-7269 AF680
200 µg/ml
$357.00

VEGF 抗体 (C-1) Alexa Fluor® 790

sc-7269 AF790
200 µg/ml
$357.00

这个抗体WB实验 ,蛋白分子量是多少?

Asked by: 科研小菜
Thanks for your questions.Molecular Weight of VEGF monomer: 21 kDa. Molecular Weight of VEGF dimer: 42 kDa. 感谢您的提问。VEGF单体分子量:21 kDa。VEGF二聚体分子量:42 kDa。
Answered by: Yanru Tao
Date published: 2024-08-08

Hello, could you tell me what antigen retrieval is used for VEGF cat# sc-7269? It does not mention the antigen retrieval in the product information. Also, the dilution is 1:50-1:500 is this correct?

Asked by: Kimberly Livingston
Thank you for your question. Please see section C of our protocol for antigen retrieval : https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining . Yes, the recommended dilution is 1:50-1:500.
Answered by: BlakeJ
Date published: 2024-07-03

Can this antibody be used in Flow Cytometry as a primary antibody?

Asked by: Sofia
Thank you for your question. Yes , the antibody can be used for Flow Cytometry. Here is our protocol: https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2023-09-19

Is this product also applicable to ELISA? If so, could you please provide the recommended concentration for coating and antigen detection?

Asked by: YHLee
Thank you for your question. This antibody is not recommended for ELISA.
Answered by: Technical Service SH
Date published: 2023-09-15

we gained intensed band at 18 kDa . Is it nonspecific band?

Asked by: jepark
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-06-12

Is this antibody derived from mouse?Dose the reactivity include mouse?

Asked by: yifan_lixi
Yes, VEGF Antibody (C-1): sc-7269 is derived from mouse. It can be used to detect mouse samples by WB, IP, IF and IHC(P).
Answered by: Technical Service SH
Date published: 2023-11-17

can i use this antibody in western blotting to detect Vegf protein in gastrocnemius and quadriceps extract of mouse, and which dilution is recommended, thanks

Asked by: DinaAli
Thank you for your question. VEGF (C-1) antibody is recommended for mouse, rat and human species. the recommended dilution is 1:100-1:1000. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2023-05-05

Can we use this (sc-7269) for IF and WB to detect VEGF-A in rat hippocampus (using either brain slices or brain tissue)? If yes, are there any published papers on this using this antibody?

Asked by: Jenn12
Thank you for your question. We have not tested this antibody for rat hippocampus but we have validated this antibody to work for mouse, rat and human.
Answered by: BlakeJ
Date published: 2023-02-23
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Rated 5 out of 5 by from Works well for WBWorks well for neonatal mouse lungs at the concentration of 30ug of protein. Ab dilution will be 1:200 for overnight at 4oC
Date published: 2024-12-13
Rated 4 out of 5 by from Great for WBThis primary antibody is useful in western blotting.
Date published: 2024-04-01
Rated 5 out of 5 by from SuperGénial, je suis dsl cependant je fais ceci pour des tickets alors ne pas faire confiance à moi!
Date published: 2024-03-25
Rated 4 out of 5 by from 기능이좋습니다.3사의 제품을 써보고 santa 것으로 쓰기로 했습니다. 여러조건으로 해본 결과 BSA로 blocking 하고 1st를 skim으로 쓰면 잘 나옵니다. 샘플로 사용해보고 본제품 구매합니다.
Date published: 2023-04-18
Rated 5 out of 5 by from WonderfulWonderful results! I used this antibody for western blot application in mouse brain samples.
Date published: 2023-02-02
Rated 5 out of 5 by from excellent antibodyA problem-free antibody that works very well in the immunohistochemistry technique in rat tissue.
Date published: 2022-12-12
Rated 5 out of 5 by from Ottimo funzionamento su tessuto di caneBuoni risultati con smascheramento a pH 9.0 e concentrazione 1:150. Testato su tessuto di granulazione, rene e fegato di specie canina.
Date published: 2022-07-22
Rated 4 out of 5 by from Buon anticorpo verso VEGFHo testato questo anticorpo su lisato di cellule endoteliali umane e funziona bene 1h a RT
Date published: 2022-04-07
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VEGF 抗体 (C-1) is rated 4.4 out of 5 by 86.
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