UDP-GlcDH抗体 (A-9) 推荐用于 mouse, rat 和 human 来源的UDP-GlcDH WB, IP, IF 和 ELISA检测

UDP-GlcDH 抗体 (A-9): sc-393438

UDP-GlcDH抗体 (A-9) is rated 5.0 out of 5 by 2.
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说明书

    • UDP-GlcDH抗体(A-9)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
    • 特异性抗原位于human物种的UDP-GlcDH的C-terminus的氨基酸459-486之间
    • 推荐用于 mouse, rat 和 human 来源的UDP-GlcDH WB, IP, IF 和 ELISA检测
    • 封闭肽,sc-393438 P
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)

What blotting conditions were used for the blot? dilution? blocking agent? I used the antibody at 1:1000 in 5% milk/TBST. It was almost clean enough but could be a little better.

Asked by: Joy N
Thank you for your question. You can find dilution recommendations for each antibody on their respective datasheets. Our protocol for western blotting that is used by our labs as well can be found under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting We recommend UltraCruz® Blocking Reagent: sc-516214. Alternatively, a milk-based blocking buffer will produce a cleaner blot while BSA will provide stronger signals. Incubating the blot at a low dilution over night at 4 °C can also help to produce a robust and specific signal in many cases.
Answered by: Tech Support Europe
Date published: 2020-07-09

What application is the blocking peptide sc-393438 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Nice Western Blot data of UDP Nice Western Blot data of UDP-GlcDH expression in Hep G2, HeLa and A-431 whole cell lysates and human liver tissue extract . -SCBT QC
Date published: 2014-04-23
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