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UDP-GlcDH Antibody (A-9) - Western Blotting - Image 295855
UDP-GlcDH 抗体 (A-9): sc-393438. Hep G2 (A), HeLa (B) 和 A-431 (C) 全细胞裂解液及人肝组织提取物 (D) 中 UDP-GlcDH 表达的 Western 印迹分析.
UDP-GlcDH Antibody (A-9) - Western Blotting - Image 295855
UDP-GlcDH 抗体 (A-9): sc-393438. Hep G2 (A), HeLa (B) 和 A-431 (C) 全细胞裂解液及人肝组织提取物 (D) 中 UDP-GlcDH 表达的 Western 印迹分析.
UDP-GlcDH Antibody (A-9) - Immunofluorescence - Image 296494
UDP-GlcDH 抗体 (A-9): sc-393438. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞核和细胞质定位.
UDP-GlcDH Antibody (A-9) - Western Blotting - Image 366912
UDP-GlcDH 抗体 (A-9): sc-393438. Hep G2 (A), A549 (B) 和 MCF7 (C) 全细胞裂解液中 UDP-GlcDH 表达的 Western 印迹分析.
UDP-GlcDH 抗体 (A-9): sc-393438. Hep G2 (A), HeLa (B) 和 A-431 (C) 全细胞裂解液及人肝组织提取物 (D) 中 UDP-GlcDH 表达的 Western 印迹分析.

UDP-GlcDH 抗体 (A-9): sc-393438

5.0(2)
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说明书
  • UDP-GlcDH抗体(A-9)是小鼠单克隆IgG1 (kappa轻链)，规格为200 µg/ml
  • 特异性抗原位于human物种的UDP-GlcDH的C-terminus的氨基酸459-486之间
  • 推荐用于 mouse, rat 和 human 来源的UDP-GlcDH WB, IP, IF 和 ELISA检测
  • 1 BP-HRP">m-IgG1 BP-HRP是UDP-GlcDH Antibody (A-9) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与UDP-GlcDH Antibody (A-9) 打包提供（请参阅下面的订购信息）。

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    研究信息

    関連項目

    附加UDP-Glucose Dehydrogenase抗体，包括UDP-GlcDH

    UDP-GlcDH（也被称为UDP-葡萄糖6-脱氢酶、UGDH或UDPGDH）是UDP-葡萄糖/GDP-甘露糖脱氢酶家族的一员。UDP-GlcDH将UDP-葡萄糖转化为UDP-葡糖醛酸，这是糖胺聚糖、透明质酸、硫酸乙酰肝素和硫酸软骨素生物合成中的关键组分。这些糖胺聚糖作为细胞外基质的常见成分，在信号转导、细胞迁移、癌症生长和癌症转移中具有重要意义。UDP-葡糖醛酸（UDP-GlcA）在肝脏中对于有毒化合物的排泄是必需的。UDP-GlcDH是一种普遍表达的蛋白质，在肝脏中含量最为丰富。UDP-GlcDH的蛋白质结构首先在牛肝中进行了分析，发现其为同源六聚体。这种结构在不同物种和门之间高度保守，不同哺乳动物物种之间的序列同一性总体上为97%。研究表明，UDP-GlcDH的表达受到TGFβ的上调和低氧的下调。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    UDP-GlcDH 抗体 (A-9)

    sc-393438
    200 µg/ml
    		$316.00

    UDP-GlcDH (A-9): m-IgG1 BP-HRP 套装

    sc-542711
    200 µg Ab; 20 µg BP
    		$354.00

    UDP-GlcDH (A-9) 中和勝肽

    sc-393438 P
    100 µg/0.5 ml
    		$68.00

    What blotting conditions were used for the blot? dilution? blocking agent? I used the antibody at 1:1000 in 5% milk/TBST. It was almost clean enough but could be a little better.

    Asked by: Joy N
    Thank you for your question. You can find dilution recommendations for each antibody on their respective datasheets. Our protocol for western blotting that is used by our labs as well can be found under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting We recommend UltraCruz® Blocking Reagent: sc-516214. Alternatively, a milk-based blocking buffer will produce a cleaner blot while BSA will provide stronger signals. Incubating the blot at a low dilution over night at 4 °C can also help to produce a robust and specific signal in many cases.
    Answered by: Tech Support Europe
    Date published: 2020-07-09

    What application is the blocking peptide sc-393438 P appropriate for?

    Asked by: Germaine
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-28
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    Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-02-23
    Rated 5 out of 5 by from Nice Western Blot data of UDPNice Western Blot data of UDP-GlcDH expression in Hep G2, HeLa and A-431 whole cell lysates and human liver tissue extract . -SCBT QC
    Date published: 2014-04-23
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    UDP-GlcDH 抗体 (A-9) is rated 5.0 out of 5 by 2.
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