UBE2N Antibody (4E11): sc-58452
- UBE2N Antibody (4E11) is a mouse monoclonal IgG1 κ UBE2N antibody, cited in 1 publications, provided at 200 µg/ml
- raised against full length UBE2N of human origin
- UBE2N Antibody (4E11) is recommended for detection of UBE2N of mouse, rat and human origin by WB, IF and ELISA
- Anti-UBE2N Antibody (4E11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for UBE2N Antibody (4E11). This work is in progress.
UBE2N Antibody (4E11) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBE2N protein of mouse, rat, and human origin by western blotting (WB), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-UBE2N antibody (4E11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBE2N, a crucial member of the E2 ubiquitin-conjugating enzyme family, plays a vital role in the ubiquitination process, which is essential for targeting proteins for proteasomal degradation. This process is particularly important for maintaining cellular homeostasis, as UBE2N regulates the levels of abnormal or short-lived proteins. UBE2N facilitates the attachment of ubiquitin, a small protein consisting of 76 amino acids, to lysine residues on target proteins, leading to the formation of polyubiquitin chains that signal for degradation by the proteasome. The efficient degradation of these proteins is critical for various cellular functions, including the regulation of gene expression, cell differentiation, and the DNA repair pathway, thereby enhancing cell survival following DNA damage. UBE2N is notably expressed in heart and skeletal muscles, where UBE2N activity is linked to muscle function and overall cellular health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBE2N Antibody (4E11) References:
- Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55(Gag). | VerPlank, L., et al. 2001. Proc Natl Acad Sci U S A. 98: 7724-9. PMID: 11427703
- Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis. | Risseeuw, EP., et al. 2003. Plant J. 34: 753-67. PMID: 12795696
- UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme. | Okamoto, Y., et al. 2003. Cancer Res. 63: 4167-73. PMID: 12874022
- Bcl10 activates the NF-kappaB pathway through ubiquitination of NEMO. | Zhou, H., et al. 2004. Nature. 427: 167-71. PMID: 14695475
- Distinct regulation of Ubc13 functions by the two ubiquitin-conjugating enzyme variants Mms2 and Uev1A. | Andersen, PL., et al. 2005. J Cell Biol. 170: 745-55. PMID: 16129784
- The ubiquitin-proteasome system. | Nandi, D., et al. 2006. J Biosci. 31: 137-55. PMID: 16595883
- [E2 ubiquitin-conjugating enzymes: structures and functions]. | Tokunaga, F. 2006. Tanpakushitsu Kakusan Koso. 51: 1150-6. PMID: 16922367
- Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells. | Sancho, E., et al. 1998. Mol Cell Biol. 18: 576-89. PMID: 9418904
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2N Antibody (4E11) | sc-58452 | 200 µg/ml | $322.00 | |||
UBE2N Antibody (4E11) AC | sc-58452 AC | 500 µg/ml, 25% agarose | $424.00 | |||
UBE2N Antibody (4E11) HRP | sc-58452 HRP | 200 µg/ml | $322.00 | |||
UBE2N Antibody (4E11) FITC | sc-58452 FITC | 200 µg/ml | $336.00 | |||
UBE2N Antibody (4E11) PE | sc-58452 PE | 200 µg/ml | $349.00 | |||
UBE2N Antibody (4E11) Alexa Fluor® 488 | sc-58452 AF488 | 200 µg/ml | $364.00 | |||
UBE2N Antibody (4E11) Alexa Fluor® 546 | sc-58452 AF546 | 200 µg/ml | $364.00 | |||
UBE2N Antibody (4E11) Alexa Fluor® 594 | sc-58452 AF594 | 200 µg/ml | $364.00 | |||
UBE2N Antibody (4E11) Alexa Fluor® 647 | sc-58452 AF647 | 200 µg/ml | $364.00 | |||
UBE2N Antibody (4E11) Alexa Fluor® 680 | sc-58452 AF680 | 200 µg/ml | $364.00 | |||
UBE2N Antibody (4E11) Alexa Fluor® 790 | sc-58452 AF790 | 200 µg/ml | $364.00 |