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Tyk 2 Antibody (C-8) - Western Blotting - Image 376135 — Tyk 2 抗体 (C-8): sc-5271. C4 (A), Neuro-2A (B) 和 C6 (C) 全细胞裂解液中 Tyk 2 表达的 Western 印迹分析.

Tyk 2 抗体 (C-8): sc-5271. C4 (A), Neuro-2A (B) 和 C6 (C) 全细胞裂解液中 Tyk 2 表达的 Western 印迹分析.

Tyk 2 抗体 (C-8): sc-5271

Name: Tyk 2抗体 (C-8)
Brand: SCBT
SKU: sc-5271
Price: 316.0 USD
Availability: InStock

4.1(19)

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说明书

Tyk 2 抗体 C-8 是小鼠单克隆 IgG₁ κ，Tyk 2抗体, 在19篇文献中引用，规格为200 µg/ml
特异性抗原位于human物种的Tyk 2的C-terminus的氨基酸1170-1187之间
Tyk 2 抗体 (C-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TYk 2
抗Tyk 2抗体（C-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取Tyk 2 (C-8): sc-5271的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP, m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 Tyk 2 抗体 (C-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Tyk 2 抗体 (C-8) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(19)

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Tyk 2 抗体 (C-8) 参考文献:

JAK/STAT 通路参与白细胞介素-17 信号转导机制的证据。 | Subramaniam, SV., et al. 1999. Biochem Biophys Res Commun. 262: 14-9. PMID: 10448060
细胞因子 II 类受体复合物眼中的 Jak-Stat 信号转导途径。 | Kotenko, SV. and Pestka, S. 2000. Oncogene. 19: 2557-65. PMID: 10851054
IFN-beta 可诱导尤文氏肉瘤细胞中的 Stat-1 发生丝氨酸磷酸化，并通过诱导 IRF-1 和激活 caspase-7 来介导细胞凋亡。 | Sancéau, J., et al. 2000. Oncogene. 19: 3372-83. PMID: 10918594
糖皮质激素抑制 IL-2 诱导的 Jak-STAT 信号传导 | Bianchi, M., et al. 2000. Proc Natl Acad Sci U S A. 97: 9573-8. PMID: 10920190
激活 JAK/STAT 通路可在大鼠急性心肌梗死中传递细胞保护信号。 | Negoro, S., et al. 2000. Cardiovasc Res. 47: 797-805. PMID: 10974228
解决自身免疫中 TYK2 基因型与表型之间的差异。 | Dendrou, CA., et al. 2016. Sci Transl Med. 8: 363ra149. PMID: 27807284
高选择性抑制酪氨酸激酶 2 (TYK2) 治疗自身免疫性疾病：发现异构抑制剂 BMS-986165。 | Wrobleski, ST., et al. 2019. J Med Chem. 62: 8973-8995. PMID: 31318208
选择性 TYK2 抑制剂 deucravacitinib 在银屑病关节炎 II 期试验中的疗效和安全性。 | Mease, PJ., et al. 2022. Ann Rheum Dis. 81: 815-822. PMID: 35241426
Tyk2 靶向免疫介导的炎症性疾病。 | Rusiñol, L. and Puig, L. 2023. Int J Mol Sci. 24: PMID: 36834806
孟德尔随机化和临床试验证据支持将 TYK2 抑制作为自身免疫性疾病的治疗靶点。 | Yuan, S., et al. 2023. EBioMedicine. 89: 104488. PMID: 36842216
v-Src转化的人胆囊腺癌细胞系中Jak-2和Tyk-2的持续激活。 | Murakami, Y., et al. 1998. J Cell Physiol. 175: 220-8. PMID: 9525481
Jaks 和 STAT：生物学意义。 | Leonard, WJ. and O'Shea, JJ. 1998. Annu Rev Immunol. 16: 293-322. PMID: 9597132

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
Tyk 2 抗体 (C-8)	sc-5271	200 µg/ml	$316.00
Tyk 2 (C-8): m-IgG Fc BP-HRP 套装	sc-528139	200 µg Ab; 10 µg BP	$354.00
Tyk 2 (C-8): m-IgGκ BP-HRP 套装	sc-520443	200 µg Ab, 40 µg BP	$354.00
Tyk 2 (C-8): m-IgG₁ BP-HRP 套装	sc-542783	200 µg Ab; 20 µg BP	$354.00
Tyk 2 抗体 (C-8) AC	sc-5271 AC	500 µg/ml, 25% agarose	$416.00
Tyk 2 抗体 (C-8) HRP	sc-5271 HRP	200 µg/ml	$316.00
Tyk 2 抗体 (C-8) FITC	sc-5271 FITC	200 µg/ml	$330.00
Tyk 2 抗体 (C-8) PE	sc-5271 PE	200 µg/ml	$343.00
Tyk 2 抗体 (C-8) Alexa Fluor^® 488	sc-5271 AF488	200 µg/ml	$357.00
Tyk 2 抗体 (C-8) Alexa Fluor^® 546	sc-5271 AF546	200 µg/ml	$357.00
Tyk 2 抗体 (C-8) Alexa Fluor^® 594	sc-5271 AF594	200 µg/ml	$357.00
Tyk 2 抗体 (C-8) Alexa Fluor^® 647	sc-5271 AF647	200 µg/ml	$357.00
Tyk 2 抗体 (C-8) Alexa Fluor^® 680	sc-5271 AF680	200 µg/ml	$357.00
Tyk 2 抗体 (C-8) Alexa Fluor^® 790	sc-5271 AF790	200 µg/ml	$357.00
Tyk 2 (C-8) 中和勝肽	sc-5271 P	100 µg/0.5 ml	$68.00

I tried to detect tyk2 band from mouse brain lysate in IB (using LI-COR) and couldn't detect any thing. I loaded 20 ug of brain lysate. What condition do I need to use to detect tyk2 band? Thanks.

Asked by: jiyoen kim

Thank you for your question. We have not tested this antibody in brain tissue extracts. For the Western blot data on our website, we load 50 ug whole cell lysate protein. The primary antibody was used at a dilution of 1:200. Please refer to our Western blotting protocol for additional details. And contact us for further Technical Support with using this antibody.

Answered by: Technical Support

Date published: 2022-03-16

I am using Tyk 2 (C-8): sc-5271 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara

We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-02-02

Rated 1 out of 5 by PaulaPaulo from Very weak in Western-blotWe had optimized WB with the previous sc-169 (C-20) Anti-TYK2 antibody and it worked perfectly. This is not a good replacement. We get faint bands at 1:500 dilution.

Date published: 2021-11-26

Rated 4 out of 5 by trash3 from works great for IP, not verified in knockout cellsI used this at 1:50 in lysates from a human T cell line for immunoprecipitation. I also used it to blot for Tyk2 in whole cell lysates and the IP samples. It worked well and yielded a single band. The only caveat is that I don't have a Tyk2 deficient control to verify that it is recognizing Tyk2.

Date published: 2018-07-22

Rated 5 out of 5 by HellenY from Excellent resultIn the experiment,Tyk 2 antibody stains very brightly in rat, the staining intensity between these cells was obvious, it is highly suitable to show cellular staining.

Date published: 2017-10-09

Rated 5 out of 5 by kinstiner from very good for IFIt works extremely well with no background and clear nuclear labeling of positive cells.Concentration was use 1:250.

Date published: 2017-08-12

Rated 5 out of 5 by sychoi from goodI can detect endogenous Tyk2 in 293 cell lysates and Tyk2 transfected 293 cell lysates.

Date published: 2017-08-04

Rated 4 out of 5 by 0s1kun from Superb immunoperoxidase cytoplasmic and membraneSuperb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human rectum tissue.

Date published: 2017-05-23

Rated 5 out of 5 by bexa1 from WBThis antibody is good for WB and the concentration I used is 1:500.

Date published: 2017-04-23

Rated 3 out of 5 by sayen from 检测效果有点弱有图有真相，在巨噬细胞里做的，条带位置大小是对的，用超敏发光液曝光的，真的很弱，可能巨噬细胞本身表达就很低

Date published: 2017-04-16

Tyk 2 抗体 (C-8) Reviews - page 2

Tyk 2 抗体 (C-8) is rated 4.1 out of 5 by 19.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	TYK2	Entrez Gene ID: 7297	19p13.2	XM_011528245, XM_011528246, XM_011528247, XM_011528249, XM_011528251, XM_011528252, NM_003331	UniProt ID: P29597	176941
Mouse	Tyk2	Entrez Gene ID: 54721	9 A3	XM_006510492, XM_006510493, XM_006510494, XM_011242579, NM_001205312, NM_018793	UniProt ID: Q9R117	N/A

Tyk 2 抗体 (C-8): sc-5271

Tyk 2 抗体 (C-8) 参考文献:

Tyk 2 抗体 (C-8) 规格

替代产品名称: Tyrosine kinase 2

产品克隆名称: C-8

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 1170-1187

抗原表位靶标: Tyk 2

抗原表位种属: human

产品反应性/检测: TYk 2

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 19篇各种科学出版物的引文

Tyk 2 抗体 (C-8)

Tyk 2 (C-8): m-IgG Fc BP-HRP 套装

Tyk 2 (C-8): m-IgGκ BP-HRP 套装

Tyk 2 (C-8): m-IgG₁ BP-HRP 套装

Tyk 2 抗体 (C-8) AC

Tyk 2 抗体 (C-8) HRP

Tyk 2 抗体 (C-8) FITC

Tyk 2 抗体 (C-8) PE

Tyk 2 抗体 (C-8) Alexa Fluor^® 488

Tyk 2 抗体 (C-8) Alexa Fluor^® 546

Tyk 2 抗体 (C-8) Alexa Fluor^® 594

Tyk 2 抗体 (C-8) Alexa Fluor^® 647

Tyk 2 抗体 (C-8) Alexa Fluor^® 680

Tyk 2 抗体 (C-8) Alexa Fluor^® 790

Tyk 2 (C-8) 中和勝肽

I tried to detect tyk2 band from mouse brain lysate in IB (using LI-COR) and couldn't detect any thing. I loaded 20 ug of brain lysate. What condition do I need to use to detect tyk2 band? Thanks.

I am using Tyk 2 (C-8): sc-5271 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Tyk 2 抗体 (C-8): sc-5271

Tyk 2 抗体 (C-8) 参考文献:

Tyk 2 抗体 (C-8) 规格

替代产品名称: Tyrosine kinase 2

产品克隆名称: C-8

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 1170-1187

抗原表位靶标: Tyk 2

抗原表位种属: human

产品反应性/检测: TYk 2

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 19篇各种科学出版物的引文

Tyk 2 抗体 (C-8)

Tyk 2 (C-8): m-IgG Fc BP-HRP 套装

Tyk 2 (C-8): m-IgGκ BP-HRP 套装

Tyk 2 (C-8): m-IgG1 BP-HRP 套装

Tyk 2 抗体 (C-8) AC

Tyk 2 抗体 (C-8) HRP

Tyk 2 抗体 (C-8) FITC

Tyk 2 抗体 (C-8) PE

Tyk 2 抗体 (C-8) Alexa Fluor® 488

Tyk 2 抗体 (C-8) Alexa Fluor® 546

Tyk 2 抗体 (C-8) Alexa Fluor® 594

Tyk 2 抗体 (C-8) Alexa Fluor® 647

Tyk 2 抗体 (C-8) Alexa Fluor® 680

Tyk 2 抗体 (C-8) Alexa Fluor® 790

Tyk 2 (C-8) 中和勝肽

I tried to detect tyk2 band from mouse brain lysate in IB (using LI-COR) and couldn't detect any thing. I loaded 20 ug of brain lysate. What condition do I need to use to detect tyk2 band? Thanks.

I am using Tyk 2 (C-8): sc-5271 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Tyk 2 (C-8): m-IgG₁ BP-HRP 套装

Tyk 2 抗体 (C-8) Alexa Fluor^® 488

Tyk 2 抗体 (C-8) Alexa Fluor^® 546

Tyk 2 抗体 (C-8) Alexa Fluor^® 594

Tyk 2 抗体 (C-8) Alexa Fluor^® 647

Tyk 2 抗体 (C-8) Alexa Fluor^® 680

Tyk 2 抗体 (C-8) Alexa Fluor^® 790