Tris-Borate-EDTA Buffer, 10X Powder Pack A ready to mix buffer reagent used for DNA and RNA polyacrylamide gel electrophoresis.

Tris-Borate-EDTA Buffer, 10X Powder Pack

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应用; A ready to mix buffer reagent used for DNA and RNA polyacrylamide gel electrophoresis
* Refer to Certificate of Analysis for lot specific data (including water content).

Tris-Borate-EDTA (TBE) buffer, 10X Powder Pack is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.
Additional forms available:
Tris-Borate-EDTA buffer, 10X (sc-296650)
Tris-Borate-EDTA buffer, 5X (sc-296652)


参考文献

1. Orbán, L., et al. 1991. Electrophoresis. 12: 233-240. PMID: 2070779
2. Wu, M., et al. 1998. BioTechniques. 24: 676-678. PMID: 9564543
3. Stellwagen, N.C. 1998. Electrophoresis. 19: 1542-1547. PMID: 9719523
4. Stellwagen, N.C., et al. 2000. Anal. Biochem. 287: 167-175. PMID: 11078596
5. Ren, J., et al. 2002. Anal Sci. 18: 469-471. PMID: 11999525
6. Biyani, M., et al. 2003. Electrophoresis. 24: 628-633. PMID: 12601730
7. Chen, Z., et al. 2005. Electrophoresis. 26: 4718-4728. PMID: 16294296
8. Zhou, P., et al. 2005. J Chromatogr A. 1083: 173-178. PMID: 16078704

用法 :
Ready for use following dilution to working concentrations. Each packet provides an adequate amount of material to make 1 L of 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5X or 10X stocks may also be added to an acrylamide/bis-acrylamide stock solution for making PAGE gels.
规划 :
10X
物理状态 :
Solid
溶解度 :
soluble in water
pH :
~8.3
保存 :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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