Tris Acetate-EDTA buffer, 10X Prepared with high-quality reagents for DNA or non-denaturing RNA agarose gel electrophoresis.

Tris Acetate-EDTA buffer, 10X

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应用; Prepared with high-quality reagents for DNA or non-denaturing RNA agarose gel electrophoresis
* Refer to Certificate of Analysis for lot specific data (including water content).

Tris Acetate-EDTA buffer, 10X concentrate is used for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers and may become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. May be used at various concentrations to study the mobility of DNA in solution. Since borate in TBE buffer (Tris-Borate-EDTA Buffer, 10X Powder Pack, sc-296651) is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample.
Additional forms available:
Tris Acetate-EDTA buffer (sc-296645)
Tris Acetate-EDTA buffer, 50X (sc-281694)


参考文献

1. Loening, U.E. 1967. Biochem. J. 102: 251-257. PMID: 5339944
2. Ogden, R.C., et al. 1987. Meth. Enzymol. 152: 61-87. PMID: 2443811
3. Hayes, V.M., et al. 1999. Nucleic Acids Res. 27: e29. PMID: 10497279
4. Stellwagen, E., et al. 2002. Electrophoresis. 23: 1935-1941. PMID: 12116139
5. Brody, J.R. and Kern, S.E. 2004. Anal. Biochem. 333: 1-13. PMID: 15351274

用法 :
Dilution of the concentrated TAE buffer produces a 1X TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
规划 :
10X
物理状态 :
concentrate
溶解度 :
Soluble in water
pH :
~8.3
保存 :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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