Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
translin CRISPR/Cas9 KO Plasmid (m): sc-423528
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- translin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the translin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: translin Antibody (C-2): sc-390472
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
translin CRISPR/Cas9 KO Plasmid (m) | sc-423528 | 20 µg | $397.00 |
Overview
Mouse Tsn encodes translin, a conserved RNA- and DNA-binding protein that forms a heteromeric complex with trax (TSNAX) and participates in post-transcriptional gene regulation. Translin has been linked to RNA transport and stability, microRNA processing via the C3PO nuclease complex, and modulation of localized translation in neuronal and germ cell contexts. Through these activities, TSN influences stress-responsive signaling and gene expression programs that shape cell proliferation and differentiation. Altered TSN-associated RNA regulatory networks have been implicated in neurobehavioral phenotypes and metabolic regulation, making Tsn a useful locus for dissecting RNA-mediated mechanisms in disease-relevant models.
translin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tsn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tsn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tsn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish translin protein expression.
This CRISPR knockout system enables efficient generation of Tsn-deficient cell models for investigation of translin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tsn exon(s) critical for translin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tsn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by translin CRISPR/Cas9 KO Plasmid (m) and translin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tsn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by translin HDR Plasmid (m) and translin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tsn homology arms to support homology-directed repair at defined Tsn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.