Date published: 2025-9-8
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Tom70 Antibody (A-8) - Western Blotting - Image 382179
Tom70 抗体 (A-8): sc-390545. HL-60 (A), WEHI-231 (B), A-673 (C) 和 BC3H1 (D) 全细胞裂解液中 Tom70 表达的 Western 印迹分析.
Tom70 抗体 (A-8): sc-390545. HL-60 (A), WEHI-231 (B), A-673 (C) 和 BC3H1 (D) 全细胞裂解液中 Tom70 表达的 Western 印迹分析.

Tom70 抗体 (A-8): sc-390545

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说明书
  • Tom70 抗体 A-8 是小鼠单克隆 IgG1 κ，Tom70抗体, 在39篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列492-608位于human物种的Tom70的C-terminus
  • Tom70 抗体 (A-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Tom70; 也和以下物种反应，包括： 和 equine and canine
  • 抗Tom70抗体（A-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tom70 (A-8): sc-390545的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Tom70 抗体 (A-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Tom70 抗体 (A-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Tom 抗体 包括 Tom1, Tom1L-1, Tom20, Tom22, Tom34, Tom40, Tom6和Tom70

Tom70抗体（A-8）是一种IgG1 κ型小鼠单克隆Tom70抗体（也被称为TOMM70抗体），可通过WB、IP、IF、IHC(P)和ELISA等方法检测来自小鼠、大鼠和人类的Tom70蛋白。Tom70抗体（A-8）既有非偶联形式的抗Tom70抗体，也有多种偶联形式的抗Tom70抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。线粒体蛋白转位酶（MPT）通过识别前蛋白的氨基末端信号序列（前序列）或前蛋白内的内部靶向结构域，将前蛋白转运至线粒体内。MPT包含多个组件，这些组件形成三个转位子，一个位于外膜（Tom40/70），两个位于内膜（Tim17/23和Tim22/54）。MPT的整体膜蛋白包括Tom70、Tom37、Tom22和Tom20。MPT依赖的转运将底物蛋白传递到由五个疏水蛋白组成的外膜通道，包括Tom40、Tom38、Tom7、Tom6和Tom5。人类Tom70基因定位于3号染色体长臂1区2带（3q12.2）。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tom70 抗体 (A-8) 参考文献:

  1. 引导蛋白质进入线粒体膜间隙的内部靶向信号。  |  Diekert, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 11752-7. PMID: 10518522
  2. 蛋白质跨膜转运  |  Agarraberes, FA. and Dice, JF. 2001. Biochim Biophys Acta. 1513: 1-24. PMID: 11427190
  3. 大鼠 TOM70 作为线粒体外膜前蛋白转运酶受体的特征。  |  Suzuki, H., et al. 2002. J Cell Sci. 115: 1895-905. PMID: 11956321
  4. 分子伴侣 Hsp90 和 Hsp70 将前蛋白传递给线粒体导入受体 Tom70。  |  Young, JC., et al. 2003. Cell. 112: 41-50. PMID: 12526792
  5. 特异于人类线粒体转运酶受体 Tom70 的 IgNAR 可变结构域的分离和特征描述。  |  Nuttall, SD., et al. 2003. Eur J Biochem. 270: 3543-54. PMID: 12919318
  6. 对富含四重肽重复的线粒体导入受体 Tom70 的生物物理分析表明，Tom70 是一种伸长的单体，具有固有的灵活性和不稳定性，并通过多态途径展开。  |  Beddoe, T., et al. 2004. J Biol Chem. 279: 46448-54. PMID: 15316022
  7. Tom70通过其抗氧化和抗凋亡特性预防糖尿病性心肌病。  |  Wang, P., et al. 2020. Hypertens Res. 43: 1047-1056. PMID: 32724135
  8. 线粒体外膜蛋白Tom70在蛋白质运输、膜接触位点和先天免疫中的作用。  |  Kreimendahl, S. and Rassow, J. 2020. Int J Mol Sci. 21: PMID: 33019591
  9. 冷应激诱导的PERK/OGT轴控制着TOM70辅助的线粒体蛋白导入和嵴的形成。  |  Latorre-Muro, P., et al. 2021. Cell Metab. 33: 598-614.e7. PMID: 33592173
  10. 基于Tom70的线粒体生物发生和衰老的转录调控。  |  Liu, Q., et al. 2022. Elife. 11: PMID: 35234609
  11. Tim 核心复合体确定了线粒体转运接触点的数量，并能在没有基质 Hsp70-Tim44 的情况下保持停滞的前蛋白。  |  Dekker, PJ., et al. 1997. EMBO J. 16: 5408-19. PMID: 9312000
  12. Tom40 是线粒体导入孔的亲水通道，用于导入前体蛋白[见注释]。  |  Hill, K., et al. 1998. Nature. 395: 516-21. PMID: 9774109

订购信息

产品名称产品编号规格价格数量收藏夹

Tom70 抗体 (A-8)

sc-390545
200 µg/ml
$316.00

Tom70 (A-8): m-IgG Fc BP-HRP 套装

sc-530185
200 µg Ab; 10 µg BP
$354.00

Tom70 (A-8): m-IgGκ BP-HRP 套装

sc-523578
200 µg Ab, 40 µg BP
$354.00

Tom70 (A-8): m-IgG1 BP-HRP 套装

sc-543912
200 µg Ab; 20 µg BP
$354.00

Tom70 抗体 (A-8) AC

sc-390545 AC
500 µg/ml, 25% agarose
$416.00

Tom70 抗体 (A-8) HRP

sc-390545 HRP
200 µg/ml
$316.00

Tom70 抗体 (A-8) FITC

sc-390545 FITC
200 µg/ml
$330.00

Tom70 抗体 (A-8) PE

sc-390545 PE
200 µg/ml
$343.00

Tom70 抗体 (A-8) Alexa Fluor® 488

sc-390545 AF488
200 µg/ml
$357.00

Tom70 抗体 (A-8) Alexa Fluor® 546

sc-390545 AF546
200 µg/ml
$357.00

Tom70 抗体 (A-8) Alexa Fluor® 594

sc-390545 AF594
200 µg/ml
$357.00

Tom70 抗体 (A-8) Alexa Fluor® 647

sc-390545 AF647
200 µg/ml
$357.00

Tom70 抗体 (A-8) Alexa Fluor® 680

sc-390545 AF680
200 µg/ml
$357.00

Tom70 抗体 (A-8) Alexa Fluor® 790

sc-390545 AF790
200 µg/ml
$357.00

What is the recommended concentration for flow cytometry.

Asked by: Pankaj
Thank you for contacting Santa Cruz Biotechnology. We recommend 1 µg normal immunoglobulins per 1 million cells. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-06-05

How much sample do you recommend loading on the gel for Western blot detection using Tom70 (A-8): sc-390545?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-07-10
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Rated 3 out of 5 by from GoodI used this antibody on fibroblast's whole cell lysate and it works. I tried two dilutions, 1:200 and 1:500 and both worked.
Date published: 2021-07-23
Rated 1 out of 5 by from postdocWB on mouse brain homogenate shows 2 bands, 20KDa band is not specific.
Date published: 2020-01-23
Rated 4 out of 5 by from great antibodywe tested this antibody in wb in dilutiion 1/250 in Hela cells. we detected one band in 70 KDa. it was a good antibody
Date published: 2019-04-01
Rated 4 out of 5 by from Good for western blotsWe tested this antibody in western blot using mouse skeletal muscle samples. The sensitivity is Ok (30ug protein sample, 1:1000 overnight)
Date published: 2017-11-07
Rated 5 out of 5 by from Great!I tried the Tom70 in my ovary cryosections mitochondrial staining and it worked very well. Good choice for mito marker.
Date published: 2017-09-29
Rated 5 out of 5 by from great featuresI use Tom70 antibody（A-8）in WB at 1:200,HCCLM3 cells,electrophoretic transfer film(PVDF) with 300mA ,30min.
Date published: 2017-09-26
Rated 5 out of 5 by from Work well for immunofluorescence in mouse oocytesThe Tom70 antibody produced nice staining of mitochondria in mouse MI oocytes. The oocytes were fixed with 4% paraformaldehyde in PIPES buffer with 100mM KCl, pH 7.2, permeabilized with 0.5% Triton X100, blocked with 2%BSA in PBS and incubated with the primary antibody 1:50 overnight. As a secondary antibody, we used donkey anti-mouse Alexa 488 antibody diluted 1:300.
Date published: 2017-09-07
Rated 5 out of 5 by from Very good in WB and IFExpected band in mouse macrophage cell line in WB, 1/300. Good signal in IF 1/50, mouse neurons, PFA-fixed.
Date published: 2017-08-24
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Tom70 抗体 (A-8) is rated 4.4 out of 5 by 13.
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