TMPRSS2抗体 (H-4) 推荐用于 mouse, rat 和 human 来源的TMPRSS2 catalytic chain WB, IP, IF, IHC(P) 和 ELISA检测

TMPRSS2 抗体 (H-4): sc-515727

TMPRSS2抗体 (H-4) is rated 4.0 out of 5 by 7.
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说明书

    • 抗-TMPRSS2 抗体 H-4 是小鼠单克隆 IgG1 κ,TMPRSS2抗体, 在26篇文献中引用,规格为200 µg/ml
    • 免疫human来源TMPRSS2内部区域的296-345氨基酸
    • 抗-TMPRSS2 抗体 (H-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 TMPRSS2 catalytic chain
    • 抗-TMPRSS2 抗体 (H-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取TMPRSS2 (H-4): sc-515727免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 TMPRSS2 Antibody (H-4) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供TMPRSS2 Antibody (H-4)。 (见下方订购信息).
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Dear technical support team, I would like to use your TMPRSS2 (H-4) -PE antibody for flow cytometric analysis. Was this antibody validated for flow cytometry and does it require cell permeabilization?

Asked by: ealeksa2
Thank you for your question. TMPRSS2 Antibody (H-4): sc-515727 was raised against amino acids 296-345 mapping within an internal region of TMPRSS2 of human origin, which correspond to the extracellular domain of the protein. The antibody has not been validated for flow cytometry.
Answered by: Technical Support Europe
Date published: 2020-10-08

Dear Technical Support Team, we hope to use the TMPRSS2 Antikörper (H-4): sc-515727 PE in flow cytometry. Which dilution/ µg of antibody do you recommend per 1 Mio cells?

Asked by: LiMS
Thank you for your question. We recommend using 1 µg antibody (5 µl) per 1 million cells.
Answered by: Tech Support Europe
Date published: 2020-08-04

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TMPRSS2 (H-4): sc-515727?

Asked by: Cweed
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Good antibody I used this antibody fo immunofluorescence in Calu-3 and Hek293 cells. I actually visualised the antibody only in Calu-3 cells, as expected. Good staining.
Date published: 2021-05-04
Rated 5 out of 5 by from Works for immunofluorescence Used it to visualize ectopically expressed TMPRSS2 in HEK293 cells by IF. Gave good signal with apparent cell surface expression, as expected. This was a courtesy replacement for sc-101847, which gave no signal at all.
Date published: 2020-12-30
Rated 4 out of 5 by from Working in WB human TMPRSS2 expressed in mice was successfully detected by WB.
Date published: 2020-11-21
Rated 4 out of 5 by from Good product and works well. I bought a month ago and it works for detecting TMPRSS2 proteins.
Date published: 2020-07-14
Rated 5 out of 5 by from Excellent in western blotting Dilution 1.1000 Western Blotting cell lysate of MDA, C2C12, HepG2 Very stong and specific signals. Detection changes according the cell type (different glycosilate forms)m
Date published: 2020-05-18
Rated 1 out of 5 by from Kein spezifisches Signal Bei der Expression in Oozyten haben wir kein spezifisches Signal in Ganzzelllysaten detektiert. Starkes Signal bei etwa 55 kDa ist unspezifisch und auch in Kontrollgruppe vorhanden.
Date published: 2020-05-18
Rated 5 out of 5 by from effective i recieved this antibody 2 momths ago for free trial, and i'm happy to got a band in the right place (1:100 dilution), band is clear
Date published: 2017-08-15
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