Date published: 2025-9-7
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TMPRSS2 Antibody (H-4) - Western Blotting - Image 396555
TMPRSS2 抗体 (H-4) Alexa Fluor® 546: sc-515727 AF546. TMPRSS2 在 Caco-2 (A), PC-3 (B), RT-4 (C) 和 LNCaP (D) 全细胞裂解液及人重组 TMPRSS2 融合蛋白 (E) 中表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
TMPRSS2 抗体 (H-4) Alexa Fluor® 546: sc-515727 AF546. TMPRSS2 在 Caco-2 (A), PC-3 (B), RT-4 (C) 和 LNCaP (D) 全细胞裂解液及人重组 TMPRSS2 融合蛋白 (E) 中表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.

TMPRSS2 抗体 (H-4): sc-515727

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说明书
  • TMPRSS2 抗体 H-4 是小鼠单克隆 IgG1 κ，TMPRSS2抗体, 在74篇文献中引用，规格为200 µg/ml
  • 免疫human来源TMPRSS2内部区域的296-345氨基酸
  • TMPRSS2 抗体 (H-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TMPRSS2 catalytic chain
  • 抗TMPRSS2抗体（H-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TMPRSS2 (H-4): sc-515727的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 TMPRSS2 抗体 (H-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TMPRSS2 抗体 (H-4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 TMPRSS 抗体 包括 TMPRSS4

TMPRSS2抗体（H-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的TMPRSS2蛋白。抗TMPRSS2抗体（H-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。TMPRSS2是一种由492个氨基酸组成的多聚丝氨酸蛋白酶，在调节细胞外基质成分方面发挥着关键作用，而细胞外基质成分对于肿瘤生长和转移等过程至关重要。TMPRSS2 (H-4)抗体可检测在小鼠前列腺和肾脏以及人类小肠、前列腺、结肠、胃和唾液腺中主要表达的蛋白质。TMPRSS2具有多个重要的结构域，包括S1家族的丝氨酸蛋白酶结构域、富半胱氨酸清除受体结构域、A类低密度脂蛋白受体结构域和跨膜结构域，这些结构域共同决定了酶的活性和功能的多功能性。TMPRSS2以全长形式和裂解蛋白酶域形式存在，其表达受雄激素上调，表明可能与前列腺癌的发生有关。抗TMPRSS2抗体（H-4）有助于研究人员研究TMPRSS2与各种生长因子的相互作用和激活机制，突出了其在正常生理过程和病理条件下的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TMPRSS2 抗体 (H-4) 参考文献:

  1. 前列腺定位和雄激素调控的膜结合丝氨酸蛋白酶 TMPRSS2 的表达。  |  Lin, B., et al. 1999. Cancer Res. 59: 4180-4. PMID: 10485450
  2. 跨膜丝氨酸蛋白酶 TMPRSS2 在小鼠和人体组织中的表达。  |  Vaarala, MH., et al. 2001. J Pathol. 193: 134-40. PMID: 11169526
  3. 雄激素调控的 TMPRSS2 蛋白酶催化裂解导致前列腺和前列腺癌上皮细胞分泌该蛋白酶。  |  Afar, DE., et al. 2001. Cancer Res. 61: 1686-92. PMID: 11245484
  4. 表达谱分析揭示了前列腺癌中hepsin的过度表达。  |  Magee, JA., et al. 2001. Cancer Res. 61: 5692-6. PMID: 11479199
  5. Tmprss2特异性miRNA有望成为SARS-CoV-2进入检查点的调节因子。  |  Kaur, T., et al. 2021. Virus Res. 294: 198275. PMID: 33359190
  6. 跨膜丝氨酸蛋白酶2（TMPRSS2）抑制剂的发现和开发，作为治疗新冠肺炎的候选药物。  |  Mantzourani, C., et al. 2022. Expert Opin Drug Discov. 17: 231-246. PMID: 35072549
  7. ACE2和TMPRSS2免疫定位与COVID-19相关的甲状腺疾病。  |  Park, GC., et al. 2022. Biology (Basel). 11: PMID: 35625425
  8. 通过乳铁蛋白阻断TMPRSS2介导的SARS-CoV-2的启动。  |  Ohradanova-Repic, A., et al. 2022. Front Immunol. 13: 958581. PMID: 36081512
  9. 开发一种3CLpro和TMPRSS2的多靶点抑制剂在对抗SARS-CoV-2方面是否具有前景？  |  Dos Santos Nascimento, IJ. and de Moura, RO. 2023. Med Chem. 19: 405-412. PMID: 36221875
  10. 人类冠状病毒HKU1识别宿主受体TMPRSS2。  |  McCallum, M., et al. 2024. Cell. 187: 4231-4245.e13. PMID: 38964328
  11. Hepsin是一种在肝癌细胞中发现的细胞表面丝氨酸蛋白酶，在卵巢癌中也有过表达。  |  Tanimoto, H., et al. 1997. Cancer Res. 57: 2884-7. PMID: 9230195
  12. 克隆 TMPRSS2 基因，该基因编码一种新型丝氨酸蛋白酶，具有跨膜, LDLRA 和 SRCR 结构域，并绘制到 21q22.3 上。  |  Paoloni-Giacobino, A., et al. 1997. Genomics. 44: 309-20. PMID: 9325052

订购信息

产品名称产品编号规格价格数量收藏夹

TMPRSS2 抗体 (H-4)

sc-515727
200 µg/ml
$316.00

TMPRSS2 (H-4): m-IgG Fc BP-HRP 套装

sc-531486
200 µg Ab; 10 µg BP
$354.00

TMPRSS2 (H-4): m-IgGκ BP-HRP 套装

sc-525184
200 µg Ab, 40 µg BP
$354.00

TMPRSS2 (H-4): m-IgG1 BP-HRP 套装

sc-544584
200 µg Ab; 20 µg BP
$354.00

TMPRSS2 抗体 (H-4) AC

sc-515727 AC
500 µg/ml, 25% agarose
$416.00

TMPRSS2 抗体 (H-4) HRP

sc-515727 HRP
200 µg/ml
$316.00

TMPRSS2 抗体 (H-4) FITC

sc-515727 FITC
200 µg/ml
$330.00

TMPRSS2 抗体 (H-4) PE

sc-515727 PE
200 µg/ml
$343.00

TMPRSS2 抗体 (H-4) Alexa Fluor® 488

sc-515727 AF488
200 µg/ml
$357.00

TMPRSS2 抗体 (H-4) Alexa Fluor® 546

sc-515727 AF546
200 µg/ml
$357.00

TMPRSS2 抗体 (H-4) Alexa Fluor® 594

sc-515727 AF594
200 µg/ml
$357.00

TMPRSS2 抗体 (H-4) Alexa Fluor® 647

sc-515727 AF647
200 µg/ml
$357.00

TMPRSS2 抗体 (H-4) Alexa Fluor® 680

sc-515727 AF680
200 µg/ml
$357.00

TMPRSS2 抗体 (H-4) Alexa Fluor® 790

sc-515727 AF790
200 µg/ml
$357.00

Would this work with Vero cells? Has it been used in them before?

Asked by: Adam UK
Thank you for your questions. Based on tests on a panel of different cell lysate and tissue samples, TMPRSS2 Antibody (H-4): sc-515727 is recommended for detection of TMPRSS2 catalytic chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA and should also detect its target protein in Vero cells if it is present in this cell type.
Answered by: Tech Support Europe
Date published: 2022-10-25

Dear technical support, Has this antibody been validated using KO or silencing? We've tested 4 different anti TMPRSS2 antibodies so far they all give a band at the right siez but 1) even in lysates from cells known not to express TMPRSS2 2) even in KO!

Asked by: CGJvirus
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Dear technical support team, I would like to use your TMPRSS2 (H-4) -PE antibody for flow cytometric analysis. Was this antibody validated for flow cytometry and does it require cell permeabilization?

Asked by: ealeksa2
Thank you for your question. TMPRSS2 Antibody (H-4): sc-515727 was raised against amino acids 296-345 mapping within an internal region of TMPRSS2 of human origin, which correspond to the extracellular domain of the protein. The antibody has not been validated for flow cytometry.
Answered by: Technical Support Europe
Date published: 2020-10-08

Dear Technical Support Team, we hope to use the TMPRSS2 Antikörper (H-4): sc-515727 PE in flow cytometry. Which dilution/ µg of antibody do you recommend per 1 Mio cells?

Asked by: LiMS
Thank you for your question. We recommend using 1 µg antibody (5 µl) per 1 million cells.
Answered by: Tech Support Europe
Date published: 2020-08-04

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TMPRSS2 (H-4): sc-515727?

Asked by: Cweed
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Good antibodyI used this antibody fo immunofluorescence in Calu-3 and Hek293 cells. I actually visualised the antibody only in Calu-3 cells, as expected. Good staining.
Date published: 2021-05-04
Rated 5 out of 5 by from Works for immunofluorescenceUsed it to visualize ectopically expressed TMPRSS2 in HEK293 cells by IF. Gave good signal with apparent cell surface expression, as expected. This was a courtesy replacement for sc-101847, which gave no signal at all.
Date published: 2020-12-30
Rated 4 out of 5 by from Working in WBhuman TMPRSS2 expressed in mice was successfully detected by WB.
Date published: 2020-11-21
Rated 4 out of 5 by from Good product and works well.I bought a month ago and it works for detecting TMPRSS2 proteins.
Date published: 2020-07-14
Rated 5 out of 5 by from Excellent in western blottingDilution 1.1000 Western Blotting cell lysate of MDA, C2C12, HepG2 Very stong and specific signals. Detection changes according the cell type (different glycosilate forms)m
Date published: 2020-05-18
Rated 1 out of 5 by from Kein spezifisches SignalBei der Expression in Oozyten haben wir kein spezifisches Signal in Ganzzelllysaten detektiert. Starkes Signal bei etwa 55 kDa ist unspezifisch und auch in Kontrollgruppe vorhanden.
Date published: 2020-05-18
Rated 5 out of 5 by from effectivei recieved this antibody 2 momths ago for free trial, and i'm happy to got a band in the right place (1:100 dilution), band is clear
Date published: 2017-08-15
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TMPRSS2 抗体 (H-4) is rated 4.0 out of 5 by 7.
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