TLR7抗体 (4F4) 推荐用于 human 来源的TLR7 WB, IP 和 IF检测

TLR7 抗体 (4F4): sc-57463

TLR7抗体 (4F4) is rated 3.5 out of 5 by 4.
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说明书

    • 抗-TLR7 抗体 4F4 是小鼠单克隆 IgG1 (kappa轻链),TLR7抗体,规格为50 µg/0.5 ml
    • 免疫human物种的TLR7的氨基酸451-500
    • 推荐用于 human 来源的TLR7 WB, IP 和 IF检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取TLR7 (4F4): sc-57463免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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Seria possível dizerem-me qual a sequência do imunógeno?

Asked by: PinhoP
Thank you for your question. This antibody was raised against amino acids 451-500 of TLR7 of human origin. The exact epitope has not been mapped, so no further information is available.
Answered by: Tech Service
Date published: 2020-02-05

I am using TLR7 (4F4): sc-57463 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 1 out of 5 by from Zeigt leider eine unspezifische Bande An mit verschiedenen TLRs stabil transduzierten HEK-Zell-Kulturen getestet. Zeigt leider in allen Lysaten eine Bande, nicht nur bei TLR7.
Date published: 2018-05-30
Rated 5 out of 5 by from Gute Ergebnisse Immunfluoreszenz Habe verschiedene Tumor-Zelllinien getestet und der AK hat super funktioniert.
Date published: 2017-12-15
Rated 5 out of 5 by from We found the product working extremely well We found the product working extremely well on cervical tissue and would recommend others working on human tissue to use the antibody.
Date published: 2015-11-11
Rated 3 out of 5 by from Signal via western blot analysis of TLR7 Signal via western blot analysis of TLR7 expression in Ramos whole cell lysate. -SCBT QC
Date published: 2015-02-13
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