Date published: 2025-9-7
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Tim23 Antibody (H-8) - Western Blotting - Image 382236
Tim23 抗体 (H-8): sc-514463. MCF7 (A), IMR-32 (B), A549 (C), AMJ2-C8 (D) 和 PC-12 (E) 全细胞裂解液中 Tim23 表达的 Western 印迹分析.
Tim23 抗体 (H-8): sc-514463. MCF7 (A), IMR-32 (B), A549 (C), AMJ2-C8 (D) 和 PC-12 (E) 全细胞裂解液中 Tim23 表达的 Western 印迹分析.

Tim23 抗体 (H-8): sc-514463

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说明书
  • Tim23 抗体 H-8 是小鼠单克隆 IgG2b κ，Tim23抗体, 在72篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列31-209位于human物种的Tim23的C-terminus
  • Tim23 抗体 (H-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Tim23
  • 抗Tim23抗体（H-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tim23 (H-8): sc-514463的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Tim23 Antibody (H-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Tim23 Antibody (H-8) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Mitochondrial Import Inner Membrane Translocase Tim抗体，包括Tim50, Tim17, Tim23, Tim44, Tim8A和Tim9A

Tim23抗体（H-8）是一种IgG2bκ小鼠单克隆Tim23抗体（也指定为Tim23抗体），可以通过WB、IP、IF和ELISA检测来自小鼠、大鼠和人类的Tim23蛋白。Tim23抗体（H-8）既有非偶联的抗Tim23抗体形式，也有多种偶联形式的抗Tim23抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。核编码前蛋白转移到线粒体基质需要Tom和Tim转位酶的协同作用，它们分别位于线粒体外膜和内膜。线粒体外膜的前蛋白转位酶（Tom）是一个多亚基蛋白，包含至少八种蛋白：四种导入受体亚基（Tom70、Tom37、Tom22和Tom20），三种小分子蛋白（Tom7、Tom6和Tom5），以及外膜通道的结构成分（Tom40）。Tom机器涉及导入受体，它们启动细胞质合成的前蛋白与外膜的结合，以及一个通用的导入孔（GIP），它促进各种前蛋白转移到线粒体中。Tim通道导入核编码的线粒体前蛋白，涉及Tim17、Tim23和Tim44三种蛋白，它们以等摩尔比例存在。Tim17表达为两种异构体，Tim17A和Tim17B，它们仅在C端序列上有所不同，与Tim23一样，这些蛋白在胎儿和成年组织中普遍存在。Tim17和Tim23是膜整合蛋白，构成内膜通道的结构元素，前蛋白通过这些通道转移。另一方面，Tim44是一种高度亲水的蛋白，它将位于基质中的HSP 70招募到前蛋白从Tim通道出现的位置。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tim23 抗体 (H-8) 参考文献:

  1. 人类线粒体内膜易位酶的遗传和结构特征。  |  Bauer, MF., et al. 1999. J Mol Biol. 289: 69-82. PMID: 10339406
  2. 线粒体导入受体 Tom20, Tom22 和 Tom70 的结合序列在携带前序的前蛋白和不可裂解的前蛋白中的分布。  |  Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
  3. 线粒体的 TIM17.23 前蛋白转运酶：蛋白质转运到基质中的组成和功能。  |  Moro, F., et al. 1999. EMBO J. 18: 3667-75. PMID: 10393182
  4. 线粒体外膜和内膜蛋白质转运酶的多亚基复合体，通过转运中间体在体内稳定。  |  Schülke, N., et al. 1999. J Biol Chem. 274: 22847-54. PMID: 10428870
  5. 线粒体外膜导入前蛋白的运输机械。  |  Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
  6. 从TOM到TIM23复合物--前体的传递。  |  Callegari, S., et al. 2020. Biol Chem. 401: 709-721. PMID: 32074073
  7. TIM23通过防止受损线粒体中OMA1介导的降解来促进PINK1的激活。  |  Akabane, S., et al. 2023. Cell Rep. 42: 112454. PMID: 37160114
  8. TIM23复合物转运线粒体蛋白的结构基础。  |  Sim, SI., et al. 2023. Nature. 621: 620-626. PMID: 37344598
  9. 线粒体前蛋白通过TOM-TIM23超复合物导入的分子途径。  |  Zhou, X., et al. 2023. Nat Struct Mol Biol. 30: 1996-2008. PMID: 37696957
  10. TOM-TIM23超复合物的形成。  |  Jain, N., et al. 2024. Methods Enzymol. 707: 3-22. PMID: 39488380
  11. 蛋白质导入线粒体。  |  Neupert, W. 1997. Annu Rev Biochem. 66: 863-917. PMID: 9242927
  12. 人类线粒体受体 Tom20 将蛋白质导入线粒体的功能分析。  |  Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929

订购信息

产品名称产品编号规格价格数量收藏夹

Tim23 抗体 (H-8)

sc-514463
200 µg/ml
$316.00

Tim23 (H-8): m-IgG Fc BP-HRP 套装

sc-531137
200 µg Ab; 10 µg BP
$354.00

Tim23 (H-8): m-IgGκ BP-HRP 套装

sc-524737
200 µg Ab, 40 µg BP
$354.00

Tim23 (H-8): m-IgG2b BP-HRP 套装

sc-550260
200 µg Ab; 10 µg BP
$354.00

Tim23 抗体 (H-8) AC

sc-514463 AC
500 µg/ml, 25% agarose
$416.00

Tim23 抗体 (H-8) HRP

sc-514463 HRP
200 µg/ml
$316.00

Tim23 抗体 (H-8) FITC

sc-514463 FITC
200 µg/ml
$330.00

Tim23 抗体 (H-8) PE

sc-514463 PE
200 µg/ml
$343.00

Tim23 抗体 (H-8) Alexa Fluor® 488

sc-514463 AF488
200 µg/ml
$357.00

Tim23 抗体 (H-8) Alexa Fluor® 546

sc-514463 AF546
200 µg/ml
$357.00

Tim23 抗体 (H-8) Alexa Fluor® 594

sc-514463 AF594
200 µg/ml
$357.00

Tim23 抗体 (H-8) Alexa Fluor® 647

sc-514463 AF647
200 µg/ml
$357.00

Tim23 抗体 (H-8) Alexa Fluor® 680

sc-514463 AF680
200 µg/ml
$357.00

Tim23 抗体 (H-8) Alexa Fluor® 790

sc-514463 AF790
200 µg/ml
$357.00

Please let me know the epitope recognised by this antibody.

Asked by: Rishith R
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not been determined. The antibody is raised against amino acids 31-209 mapping at the C-terminus of Tim23 of human origin (Uniprot accession number O14925).
Answered by: Technical Support
Date published: 2018-12-21

Has this antibody worked on immunohistochemistry in paraffin-embeded sections of human tissue? Thank you very much!

Asked by: sanib
Thank you for your question. We have not yet tested this mouse monoclonal antibody, Tim23 Antibody (H-8): sc-514463, for Immunohistochemistry on paraffin embedded tissues, and so this application is not recommended at this time. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-11-09

How much is it in a regular vial? It says on the website that it is 200 µg/ml, but µg/ml is just a concentration. What I mean is "how much µg of antibodies in a regular vial for this antibody"? I am looking forward to your reply. Thank you.

Asked by: Susanna Su
Thank you for your question. We provide this antibody as 200 µg IgG in 1 mL total volume.
Answered by: Technical Support
Date published: 2017-09-08

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-17
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Rated 5 out of 5 by from Tim23 Antibody (H-8)Tim23 Antibody (H-8) was working very well in our western blotting.
Date published: 2024-03-21
Rated 1 out of 5 by from I didn't get the band on WB...Unfortunately, I didn't get the band on WB. I used it on H9c2, a rat cardiomyoblast, at 1:500. Your TOM40 (D2) antibody worked wonderfully with the same mitochondrial lysate...
Date published: 2024-02-06
Rated 2 out of 5 by from Not satisfiedI tested this antibody in wb in dilutiion 1/100 in fibroblast's and I detected the specific band for Tim23, however I usd the antibody diluted at 1:100.
Date published: 2021-07-23
Rated 5 out of 5 by from greatone clean band at predicted molecular weight with western blot
Date published: 2020-02-06
Rated 5 out of 5 by from Works perfect in Mice and Rats CardiomyocyteIt gives strong band in Mice and Rat Cardiomyocyte Westernblotting.
Date published: 2019-04-16
Rated 5 out of 5 by from Anticorps très fonctionnel.L'anticorps est spécifique de TIM23 par Western Blot (pas de bandes contaminantes observables).
Date published: 2018-01-08
Rated 5 out of 5 by from Works well in wbWorks well for isolated mitochondria from HEK cells. Dilution used 1:2000 for 3 hr at room temperature followed by secondary goat anti mus IgG Alexa680 1:10000 for 1 hr at room temp.
Date published: 2017-12-22
Rated 5 out of 5 by from Works great!Tried it on two membranes, and I see storong signal with the right size.
Date published: 2017-11-17
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Tim23 抗体 (H-8) is rated 4.5 out of 5 by 15.
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