TGFβ2 Double Nickase Plasmid (h): sc-400496-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TGFβ2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TGFβ2 Double Nickase Plasmid (h) and TGFβ2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TGFB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TGFβ2 Antibody (H-6): sc-374659
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGFβ2 Double Nickase Plasmid (h) | sc-400496-NIC | 20 µg | $410.00 | |||
TGFβ2 Double Nickase Plasmid (h2) | sc-400496-NIC-2 | 20 µg | $410.00 |
TGFB2 encodes transforming growth factor beta 2 (TGFβ2), a secreted cytokine that signals through type I/II serine–threonine kinase receptors to activate SMAD2/3-dependent transcription and non-canonical pathways such as MAPK, PI3K/AKT, and Rho-family GTPases. TGFβ2 regulates cell proliferation, differentiation, apoptosis, immune modulation, and extracellular matrix remodeling, contributing to tissue development and homeostasis. Dysregulated TGFB2/TGFβ2 signaling is linked to altered epithelial–mesenchymal transitions, fibrosis-associated remodeling, angiogenic programs, and immune evasion phenotypes observed across diverse disease contexts. In cancer biology and regenerative studies, TGFB2 is frequently investigated for its roles in stromal–tumor crosstalk, matrix deposition, and lineage specification.
TGFβ2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TGFB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TGFB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TGFB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TGFB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.