Date published: 2025-9-6

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Testican-2 抗体 (B-5): sc-515691

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说明书
  • Testican-2 抗体 B-5 是小鼠单克隆 IgG2b κ,Testican-2抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫human来源Testican-2近C-terminus的293-340氨基酸
  • Testican-2 抗体 (B-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Testican-2
  • 抗Testican-2抗体(B-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Testican-2 (B-5): sc-515691免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是Testican-2 Antibody (B-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Testican-2 Antibody (B-5) 打包提供(请参阅下面的订购信息)。

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    関連項目

    Testican-2抗体(B-5)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Testican-2。Testican-2 (B-5) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Testican家族(又称BM-40/SPARC/osteonectin家族)由高度保守的细胞外钙结合硫酸蛋白多糖组成,在细胞过程中发挥着关键作用。Testican-2主要在中枢神经系统(CNS)中表达,在嗅球、大脑皮层、丘脑、海马、小脑和延髓以及肺和睾丸组织中大量存在。值得注意的是,Testican-2在其家族成员中是独一无二的,因为它不仅抑制膜型基质金属蛋白酶(MT-MMPs),还能抵消其他Testican家族成员对促MMP-2活性的抑制作用。这种独特的功能对于调节细胞外基质重塑和神经突生长至关重要,因为Testican-2已被证明可以阻止小脑神经元的神经突延伸,表明它参与了中枢神经系统的发展和可塑性。Testican-2能够与N-Tes(Testican-3的氨基末端剪接变体)的COOH末端细胞外钙结合域结合,进一步强调了其在控制神经元发育和功能的复杂信号通路中的调节作用。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Testican-2 抗体 (B-5) 参考文献:

    1. 睾丸蛋白-2 的分子克隆:定义大脑中表达的新型钙结合蛋白多糖家族。  |  Vannahme, C., et al. 1999. J Neurochem. 73: 12-20. PMID: 10386950
    2. 睾丸素 2 可削弱其他睾丸素家族蛋白对膜型基质金属蛋白酶的抑制作用。  |  Nakada, M., et al. 2003. Cancer Res. 63: 3364-9. PMID: 12810672
    3. 小鼠睾丸蛋白-2。表达, 糖基化以及对神经元生长的影响。  |  Schnepp, A., et al. 2005. J Biol Chem. 280: 11274-80. PMID: 15657052
    4. 新型 PHD 手指蛋白 SPOC1:与卵巢癌残留疾病和存活率的关系。  |  Mohrmann, G., et al. 2005. Int J Cancer. 116: 547-54. PMID: 15825179
    5. 睾丸蛋白甲状腺球蛋白 1 型结构域的双重浓度依赖性活性:酪蛋白酶 L 的特异性阻抑剂和底物。  |  Meh, P., et al. 2005. Biol Chem. 386: 75-83. PMID: 15843150
    6. 睾丸素-1对于小鼠的发育是不可或缺的。  |  Röll, S., et al. 2006. Matrix Biol. 25: 373-81. PMID: 16806869

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Testican-2 抗体 (B-5)

    sc-515691
    200 µg/ml
    $316.00

    Testican-2 (B-5): m-IgG2b BP-HRP 套装

    sc-548822
    200 µg Ab; 10 µg BP
    $354.00

    Testican-2 抗体 (B-5) AC

    sc-515691 AC
    500 µg/ml, 25% agarose
    $416.00

    Testican-2 抗体 (B-5) HRP

    sc-515691 HRP
    200 µg/ml
    $316.00

    Testican-2 抗体 (B-5) FITC

    sc-515691 FITC
    200 µg/ml
    $330.00

    Testican-2 抗体 (B-5) PE

    sc-515691 PE
    200 µg/ml
    $343.00

    Testican-2 抗体 (B-5) Alexa Fluor® 488

    sc-515691 AF488
    200 µg/ml
    $357.00

    Testican-2 抗体 (B-5) Alexa Fluor® 546

    sc-515691 AF546
    200 µg/ml
    $357.00

    Testican-2 抗体 (B-5) Alexa Fluor® 594

    sc-515691 AF594
    200 µg/ml
    $357.00

    Testican-2 抗体 (B-5) Alexa Fluor® 647

    sc-515691 AF647
    200 µg/ml
    $357.00

    Testican-2 抗体 (B-5) Alexa Fluor® 680

    sc-515691 AF680
    200 µg/ml
    $357.00

    Testican-2 抗体 (B-5) Alexa Fluor® 790

    sc-515691 AF790
    200 µg/ml
    $357.00

    How much of the Testican-2 antibody do you recommend using for ELISA? Thank you!

    Asked by: AAAJ
    Thank you for your question. We don't have a recommended dilution for ELISA assay. It's best to optimize your antibody amount by titration. Please contact Technical Service for further assistance.
    Answered by: Technical Service 3
    Date published: 2020-11-12

    What's the immunogen of the Testican-2 antibody

    Asked by: Don Wen
    Thank you for your question. Testican-2 Antibody (B-5): sc-515691 has been raised against amino acids 293-340 mapping near the C-terminus of Testican-2 of human origin.
    Answered by: Tech Support Europe
    Date published: 2020-08-10

    How much sample do you recommend loading on the gel for Western blot detection using Testican-2 (B-5): sc-515691?

    Asked by: Randy McDonald
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2025, m_8, d_28, h_5CST
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    Rated 5 out of 5 by from Good specificity for Testican-2 by Western BlotAntibody detects over-expressed human Testican-2 in a transfected lysate. Shown with Cruz Marker molecular weight standards, sc-2035. -SCBT QC
    Date published: 2023-09-14
    Rated 4 out of 5 by from good antibodyI used this antibody for WB on human blood samples, it works well.
    Date published: 2017-10-23
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    Testican-2 抗体 (B-5) is rated 4.5 out of 5 by 2.
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