TBX1 Double Nickase Plasmid (h): sc-405763-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TBX1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TBX1 Double Nickase Plasmid (h) and TBX1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TBX1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TBX1 Double Nickase Plasmid (h) | sc-405763-NIC | 20 µg | $410.00 | |||
TBX1 Double Nickase Plasmid (h2) | sc-405763-NIC-2 | 20 µg | $410.00 |
TBX1 encodes a T-box transcription factor that regulates gene expression programs essential for embryonic patterning, particularly in pharyngeal apparatus and cardiopharyngeal development. TBX1 functions in the nucleus to coordinate lineage specification and morphogenesis through transcriptional control of developmental signaling networks, including interactions with pathways such as FGF and retinoic acid signaling. Dysregulation or haploinsufficiency of TBX1 is strongly associated with 22q11.2 deletion syndrome/DiGeorge spectrum phenotypes, including congenital heart defects and craniofacial anomalies. As a developmental regulator, TBX1 is widely studied to dissect gene regulatory circuits controlling cell fate decisions and tissue morphogenesis.
TBX1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TBX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TBX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TBX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TBX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.