Date published: 2025-9-10

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TBP 抗体 (A-9): sc-74596

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说明书
  • TBP 抗体 A-9 是小鼠单克隆 IgG2b κ,TBP抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫Saccharomyces cerevisiae物种的代表全长TBP的氨基酸1-240
  • TBP 抗体 (A-9) 推荐用于WB, IP, IF 和 ELISA,检测y 来源的 TBP
  • 抗TBP抗体(A-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TBP (A-9): sc-74596免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是TBP Antibody (A-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与TBP Antibody (A-9) 打包提供(请参阅下面的订购信息)。

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関連項目

TBP抗体(A-9)是一种IgG2bκ小鼠单克隆TBP抗体(也称为TF2D抗体、GTF2D1抗体或TATA结合蛋白TBP抗体),可通过WB、IP、IF和ELISA检测酿酒酵母来源的TBP蛋白。TBP抗体(A-9)既可作为非偶联抗TBP抗体形式提供,也可作为多种偶联形式的抗TBP抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在真核系统中,从蛋白质编码基因开始转录是一个复杂的过程,需要RNA聚合酶II和广泛的辅助转录因子家族。这些因子可分为两个主要的功能类别:所有Pol II基因转录所需的基础因子,包括TFIIA、TFIIB、TFIID、TFIIE、TFIIF和TFIIH;以及调节基因表达的序列特异性因子。基础转录因子和Pol II通过在转录起始位点上游通常位于25-30个碱基对的TATA框等核心启动子元件相互作用,在转录起始位点附近形成一个特定的多蛋白复合物。TFIID与TATA元件的结合启动其他因子组装成预启动复合物。来自高等真核生物的TFIID的TATA结合亚基(指定为TFIIDt或TBP)包含一个高度保守的180个氨基酸的C端结构域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TBP 抗体 (A-9) 参考文献:

  1. TFIIA 通过与基本重复序列相互作用,诱导 TFIID 发生构象变化。  |  Lee, DK., et al. 1992. Mol Cell Biol. 12: 5189-96. PMID: 1406690
  2. 鉴定人类 TFIID 成分以及 250 kDa 多肽与 TATA 盒结合蛋白(TFIID tau)之间的直接相互作用。  |  Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
  3. 人类通用转录因子 IIE 的结构和功能特性。  |  Peterson, MG., et al. 1991. Nature. 354: 369-73. PMID: 1956398
  4. 哺乳动物 RNA 聚合酶 II 参与特异性转录的因子:转录因子 IIA, IID 和 IIB 在转录功能复合体形成过程中的作用。  |  Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058

订购信息

产品名称产品编号规格价格数量收藏夹

TBP 抗体 (A-9)

sc-74596
200 µg/ml
$316.00

TBP (A-9): m-IgGκ BP-HRP 套装

sc-521152
200 µg Ab, 40 µg BP
$354.00

TBP (A-9): m-IgG2b BP-HRP 套装

sc-548866
200 µg Ab; 10 µg BP
$354.00

TBP 抗体 (A-9) AC

sc-74596 AC
500 µg/ml, 25% agarose
$416.00

TBP 抗体 (A-9) HRP

sc-74596 HRP
200 µg/ml
$316.00

TBP 抗体 (A-9) FITC

sc-74596 FITC
200 µg/ml
$330.00

TBP 抗体 (A-9) PE

sc-74596 PE
200 µg/ml
$343.00

TBP 抗体 (A-9) Alexa Fluor® 488

sc-74596 AF488
200 µg/ml
$357.00

TBP 抗体 (A-9) Alexa Fluor® 546

sc-74596 AF546
200 µg/ml
$357.00

TBP 抗体 (A-9) Alexa Fluor® 594

sc-74596 AF594
200 µg/ml
$357.00

TBP 抗体 (A-9) Alexa Fluor® 647

sc-74596 AF647
200 µg/ml
$357.00

TBP 抗体 (A-9) Alexa Fluor® 680

sc-74596 AF680
200 µg/ml
$357.00

TBP 抗体 (A-9) Alexa Fluor® 790

sc-74596 AF790
200 µg/ml
$357.00

Has this TBP antibody been used successfully for ChIP studies?

Asked by: CraigP
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-07

Hi, I am interesting about your product: TBP Antibody (A-9) Alexa Fluor® 488 sc-74596 AF488 Does this one also offers a FREE 10 µg sample for trial?  or it's just available for regular TBP (A-9): sc-74596? Regards

Asked by: JackLiao
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

What is the recommended starting dilution for this antibody sc-54796 for western blot?

Asked by: Marian 14
Thank you for your question. The recommended starting dilution is 1:200 for this antibody. Optimal conditions need to be optimized for your samples. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com or contact your local distributor.
Answered by: Tech Service
Date published: 2017-08-14

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TBP (A-9): sc-74596?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from GOOD ANTIBODY FROM SANTA CRUZThis antibody worked great for us and showed good result
Date published: 2018-03-23
Rated 5 out of 5 by from Nice Western blot data of yeast recombinantNice Western blot data of yeast recombinant TBP fusion protein. -SCBT QC
Date published: 2013-05-17
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TBP 抗体 (A-9) is rated 4.5 out of 5 by 2.
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