Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
TAUT Antibody (A-11) - Western Blotting - Image 291996
TAUT 抗体 (A-11): sc-393036. Jurkat (A) 和 Y79 (B) 全细胞裂解液中 TAUT 表达的 Western 印迹分析.
TAUT Antibody (A-11) - Western Blotting - Image 291996
TAUT 抗体 (A-11): sc-393036. Jurkat (A) 和 Y79 (B) 全细胞裂解液中 TAUT 表达的 Western 印迹分析.
TAUT Antibody (A-11) - Western Blotting - Image 393082
TAUT 抗体 (A-11): sc-393036. 非转染: sc-117752 (A) 和小鼠 TAUT 转染 sc-123925 (B) 293T 全细胞裂解液中 TAUT 表达的 Western 印迹分析.
TAUT Antibody (A-11) - Western Blotting - Image 393083
TAUT 抗体 (A-11): sc-393036. 非转染: sc-117752 (A) 和人 TAUT 转染: sc-112032 (B) 293T 全细胞裂解液中 TAUT 表达的 Western 印迹分析.
TAUT 抗体 (A-11): sc-393036. Jurkat (A) 和 Y79 (B) 全细胞裂解液中 TAUT 表达的 Western 印迹分析.

TAUT 抗体 (A-11): sc-393036

3.4(5)
寫評論提問

说明书
  • TAUT 抗体 A-11 是小鼠单克隆 IgG1 κ，TAUT抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-52位于human物种的TAUT的N-terminus
  • TAUT 抗体 (A-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 TAUT; 也和以下物种反应，包括： 和 equine and canine
  • 抗TAUT抗体（A-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TAUT (A-11): sc-393036的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成TAUT 抗体 (A-11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

TAUT抗体（A-11）是一种IgG1 κ小鼠单克隆TAUT抗体（也称为TAUT抗体），可通过WB、IP、IF和ELISA检测到小鼠、大鼠和人类来源的TAUT蛋白。TAUT抗体（A-11）既可以是非偶联的抗TAUT抗体形式，也可以是多种偶联形式的抗TAUT抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。牛磺酸是一种丰富的有机渗透压调节物质，具有抗氧化和免疫调节作用，并在细胞体积稳态中发挥作用。牛磺酸通过牛磺酸转运体（TAUT）进入细胞。TAUT依赖于钠和氯，是一种多跨膜蛋白，属于钠神经递质同向转运体（SNF）蛋白家族。TNFα上调TAUT的表达，而丝氨酸322的磷酸化则下调其表达。TAUT的过度表达可以保护肾细胞免受顺铂诱导的肾毒性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TAUT 抗体 (A-11) 参考文献:

  1. 肾细胞中p53对牛磺酸转运蛋白基因（TauT）的转录抑制。  |  Han, X., et al. 2002. J Biol Chem. 277: 39266-73. PMID: 12163498
  2. 牛磺酸转运体 TauT 在小鼠 NIH3T3 成纤维细胞中的表达和亚细胞定域调控。  |  Voss, JW., et al. 2004. Eur J Biochem. 271: 4646-58. PMID: 15606752
  3. 3T3-L1 脂肪细胞中牛磺酸转运体和牛磺酸生物合成酶的生理意义。  |  Takasaki, M., et al. 2004. Biofactors. 21: 419-21. PMID: 15630240
  4. 标准血液透析治疗对血液白细胞中人血清和糖皮质激素诱导激酶 SGK1 和牛磺酸转运体 TAUT 转录的影响。  |  Friedrich, B., et al. 2005. Nephrol Dial Transplant. 20: 768-74. PMID: 15701671
  5. 牛磺酸转运蛋白TauT在NIH3T3成纤维细胞原纤毛中的高表达。  |  Christensen, ST., et al. 2005. Cell Biol Int. 29: 347-51. PMID: 15914036
  6. 编码牛磺酸转运蛋白TAUT的人类SLC6A6的双等位基因突变与早期视网膜变性有关。  |  Preising, MN., et al. 2019. FASEB J. 33: 11507-11527. PMID: 31345061
  7. TauT/SLC6A6参与血睾屏障的牛磺酸转运。  |  Kubo, Y., et al. 2022. Metabolites. 12: PMID: 35050188
  8. 哺乳动物单羧酸转运体7（MCT7/Slc16a6）是一种新型的辅助性牛磺酸转运体。  |  Higuchi, K., et al. 2022. J Biol Chem. 298: 101800. PMID: 35257743
  9. 牛磺酸转运体的结构研究：癌症治疗中GABA转运子亚家族的一个潜在生物靶标。  |  Stary, D. and Bajda, M. 2024. Int J Mol Sci. 25: PMID: 39000444
  10. 克隆的人类胎盘牛磺酸转运体的功能特征和染色体定域。  |  Ramamoorthy, S., et al. 1994. Biochem J. 300 (Pt 3): 893-900. PMID: 8010975
  11. 人类牛磺酸转运体的克隆及甲状腺细胞摄取牛磺酸的特征。  |  Jhiang, SM., et al. 1993. FEBS Lett. 318: 139-44. PMID: 8382624
  12. 分离出编码人视网膜色素上皮细胞中牛磺酸转运体的 cDNA。  |  Miyamoto, Y., et al. 1996. Curr Eye Res. 15: 345-9. PMID: 8654117

订购信息

产品名称产品编号规格价格数量收藏夹

TAUT 抗体 (A-11)

sc-393036
200 µg/ml
$316.00

TAUT 抗体 (A-11) AC

sc-393036 AC
500 µg/ml, 25% agarose
$416.00

TAUT 抗体 (A-11) HRP

sc-393036 HRP
200 µg/ml
$316.00

TAUT 抗体 (A-11) FITC

sc-393036 FITC
200 µg/ml
$330.00

TAUT 抗体 (A-11) PE

sc-393036 PE
200 µg/ml
$343.00

TAUT 抗体 (A-11) Alexa Fluor® 488

sc-393036 AF488
200 µg/ml
$357.00

TAUT 抗体 (A-11) Alexa Fluor® 546

sc-393036 AF546
200 µg/ml
$357.00

TAUT 抗体 (A-11) Alexa Fluor® 594

sc-393036 AF594
200 µg/ml
$357.00

TAUT 抗体 (A-11) Alexa Fluor® 647

sc-393036 AF647
200 µg/ml
$357.00

TAUT 抗体 (A-11) Alexa Fluor® 680

sc-393036 AF680
200 µg/ml
$357.00

TAUT 抗体 (A-11) Alexa Fluor® 790

sc-393036 AF790
200 µg/ml
$357.00

Is it necessary to boil the samples prepared in a loading buffer for 10 min at 70 °C？Or other boiling conditions？

Asked by: qwertyu
Thank you for your question. Generally, the denaturation condition involves boiling at 95 degrees Celsius for approximately 5 minutes. For multiple transmembrane proteins, special attention needs to be paid to the denaturation conditions of samples when performing WB (Western blot) experiments, as these proteins are prone to aggregation at high temperatures, forming dimers or oligomers that increase their molecular weight, potentially leading to undetected bands or bands appearing at incorrect positions during subsequent detection. Depending on the specific characteristics of the protein, moderate thermal denaturation can be performed within a temperature range of 50°C to 70°C. Some proteins are highly sensitive to heat and have special requirements for the temperature of thermal denaturation; within this temperature range, experimentation can be conducted to find the most suitable denaturation temperature.
Answered by: Ling Chen
Date published: 2025-01-13

Are there any issues with using sc-393036: TAUT (A-11) mouse monoclonal primary antibody on mouse samples?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-393036 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_393036, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 117ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from TAUT antibody does not work in WBThis TAUT antibody does not work in WB with human liver, mouse liver, mouse kidney, and mouse brain parts. One of the test is shown here.
Date published: 2017-10-31
Rated 5 out of 5 by from Works really well in WBWB on 10µg of total mouse heart protein extract. Gel 10%, single band detected at 1/1000 and 1/10000 for secondary Ab
Date published: 2017-10-21
Rated 1 out of 5 by from IHC Assay Review for TAUT mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from We tried this antibody in WB and got cleanWe tried this antibody in WB and got clean band at 1:500 dilution.
Date published: 2015-09-03
Rated 5 out of 5 by from Produced positive Western blot data of TAUTProduced positive Western blot data of TAUT expression in Jurkat and Y79 whole cell lysates. -SCBT QC
Date published: 2013-07-16
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_393036, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
TAUT 抗体 (A-11) is rated 3.4 out of 5 by 5.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_393036, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 115ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。