TAF I p110/p95 Antibody (D-8): sc-376802
- TAF I p110/p95 Antibody (D-8) is a mouse monoclonal IgG1 κ TAF I p110/p95 antibody provided at 200 µg/ml
- raised against amino acids 570-869 mapping at the C-terminus of TAF I p110 of human origin
- TAF I p110/p95 Antibody (D-8) is recommended for detection of TAF I p110 of human origin and TAF I p95 of mouse and rat origin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TAF I p110/p95 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-376802 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TAF I p110/p95 Antibody (D-8) for WB applications. These reagents are now offered in bundles with TAF I p110/p95 Antibody (D-8) (see ordering information below).
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TAF I p110/p95 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects TAF I p110/p95 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TAF I p110/p95 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TAF I p110/p95 plays a crucial role in gene transcription regulation, particularly in assembling initiation complexes at the promoter for RNA polymerase I, where TAF I p110/p95 is a key component of human transcription factor SL1 (also known as mouse TIF-IB). This protein complex is essential for accurate ribosomal RNA transcription, vital for ribosome biogenesis and protein synthesis in cells. TAF I p110/p95, along with other TAF subunits, ensures transcriptional regulation specificity and efficiency, highlighting TAF I p110/p95′s importance in cellular growth and function. Mutually exclusive binding of TAF I or TAF II subunits to TATA-binding protein (TBP) influences promoter- and RNA polymerase-specific complex formation, further underscoring TAF I p110/p95′s significance in transcriptional control.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TAF I p110/p95 Antibody (D-8) References:
- The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1. | Comai, L., et al. 1992. Cell. 68: 965-76. PMID: 1547496
- Assembly of alternative multiprotein complexes directs rRNA promoter selectivity. | Bell, SP., et al. 1990. Genes Dev. 4: 943-54. PMID: 2384215
- A purified transcription factor (TIF-IB) binds to essential sequences of the mouse rDNA promoter. | Clos, J., et al. 1986. Proc Natl Acad Sci U S A. 83: 604-8. PMID: 3456157
- Purification and characterization of a transcription factor that confers promoter specificity to human RNA polymerase I. | Learned, RM., et al. 1985. Mol Cell Biol. 5: 1358-69. PMID: 3929071
- Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits. | Comai, L., et al. 1994. Science. 266: 1966-72. PMID: 7801123
- Assembly of transcriptionally active RNA polymerase I initiation factor SL1 from recombinant subunits. | Zomerdijk, JC., et al. 1994. Science. 266: 2015-8. PMID: 7801130
- A TBP-containing multiprotein complex (TIF-IB) mediates transcription specificity of murine RNA polymerase I. | Eberhard, D., et al. 1993. Nucleic Acids Res. 21: 4180-6. PMID: 8414971
- Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1. | Heix, J., et al. 1997. Proc Natl Acad Sci U S A. 94: 1733-8. PMID: 9050847
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAF I p110/p95 Antibody (D-8) | sc-376802 | 200 µg/ml | $322.00 | |||
TAF I p110/p95 Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-529937 | 200 µg Ab; 10 µg BP | $361.00 | |||
TAF I p110/p95 Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-523100 | 200 µg Ab, 40 µg BP | $361.00 | |||
TAF I p110/p95 Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-543774 | 200 µg Ab; 20 µg BP | $361.00 | |||
TAF I p110/p95 Antibody (D-8) X | sc-376802 X | 200 µg/0.1 ml | $322.00 | |||
TAF I p110/p95 Antibody (D-8) AC | sc-376802 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TAF I p110/p95 Antibody (D-8) HRP | sc-376802 HRP | 200 µg/ml | $322.00 | |||
TAF I p110/p95 Antibody (D-8) FITC | sc-376802 FITC | 200 µg/ml | $336.00 | |||
TAF I p110/p95 Antibody (D-8) PE | sc-376802 PE | 200 µg/ml | $349.00 | |||
TAF I p110/p95 Antibody (D-8) Alexa Fluor® 488 | sc-376802 AF488 | 200 µg/ml | $364.00 | |||
TAF I p110/p95 Antibody (D-8) Alexa Fluor® 546 | sc-376802 AF546 | 200 µg/ml | $364.00 | |||
TAF I p110/p95 Antibody (D-8) Alexa Fluor® 594 | sc-376802 AF594 | 200 µg/ml | $364.00 | |||
TAF I p110/p95 Antibody (D-8) Alexa Fluor® 647 | sc-376802 AF647 | 200 µg/ml | $364.00 | |||
TAF I p110/p95 Antibody (D-8) Alexa Fluor® 680 | sc-376802 AF680 | 200 µg/ml | $364.00 | |||
TAF I p110/p95 Antibody (D-8) Alexa Fluor® 790 | sc-376802 AF790 | 200 µg/ml | $364.00 |