SRp20 Antibody (C-1): sc-365772
- SRp20 Antibody (C-1) is a mouse monoclonal IgG3 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 131-159 near the C-terminus of SRp20 of human origin
- recommended for detection of SRp20 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- See SR (1H4): sc-13509 for SRp20 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for SRp20 Antibody (C-1) for WB applications. This reagent is now offered in a bundle with SRp20 Antibody (C-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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SRp20 Antibody (C-1) is a mouse monoclonal IgG3 antibody that detects SRp20 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRp20 is a crucial member of the serine/arginine-rich (SR) protein family, which plays a significant role in pre-mRNA splicing by binding to splicing enhancer elements and facilitating the recognition of weak splice sites. SRp20 is predominantly located in the nucleus, where SRp20 is essential for the regulation of alternative splicing, a process that allows a single gene to produce multiple protein isoforms, thereby increasing the diversity of the proteome. Nuclear localization of SRp20 enables interaction with other splicing factors and RNA, ensuring accurate splicing and mRNA maturation. SRp20 shuttles between the nucleus and cytoplasm, contributing to mRNA transport, which is critical for gene expression regulation. Post-translational modifications, such as phosphorylation, can influence SRp20 activity and interactions with other proteins, further emphasizing SRp20′s importance in the splicing machinery. With SRp20′s ability to modulate splicing events and involvement in mRNA transport, anti-SRp20 antibody (C-1) serves as an invaluable tool for researchers studying splicing mechanisms and related cellular processes.
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SRp20 Antibody (C-1) References:
- The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. | Cavaloc, Y., et al. 1999. RNA. 5: 468-83. PMID: 10094314
- The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20. | Galiana-Arnoux, D., et al. 2003. J Biol Chem. 278: 32943-53. PMID: 12826680
- RNA polymerase II C-terminal domain mediates regulation of alternative splicing by SRp20. | de la Mata, M. and Kornblihtt, AR. 2006. Nat Struct Mol Biol. 13: 973-80. PMID: 17028590
- Increased expression of splicing factor SRp20 mRNA in bipolar disorder patients. | Watanuki, T., et al. 2008. J Affect Disord. 110: 62-9. PMID: 18281098
- SRp20 and CUG-BP1 modulate insulin receptor exon 11 alternative splicing. | Sen, S., et al. 2009. Mol Cell Biol. 29: 871-80. PMID: 19047369
- Epstein-Barr Virus SM protein utilizes cellular splicing factor SRp20 to mediate alternative splicing. | Verma, D., et al. 2010. J Virol. 84: 11781-9. PMID: 20810723
- Knockdown of splicing factor SRp20 causes apoptosis in ovarian cancer cells and its expression is associated with malignancy of epithelial ovarian cancer. | He, X., et al. 2011. Oncogene. 30: 356-65. PMID: 20856201
- Epstein-Barr virus protein EB2 stimulates cytoplasmic mRNA accumulation by counteracting the deleterious effects of SRp20 on viral mRNAs. | Juillard, F., et al. 2012. Nucleic Acids Res. 40: 6834-49. PMID: 22505578
- Poliovirus infection induces the co-localization of cellular protein SRp20 with TIA-1, a cytoplasmic stress granule protein. | Fitzgerald, KD. and Semler, BL. 2013. Virus Res. 176: 223-31. PMID: 23830997
- Regulation of alternative polyadenylation by U1 snRNPs and SRp20. | Lou, H., et al. 1998. Mol Cell Biol. 18: 4977-85. PMID: 9710581
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRp20 Antibody (C-1) | sc-365772 | 200 µg/ml | $322.00 | |||
SRp20 Antibody (C-1): m-IgGκ BP-HRP Bundle | sc-522360 | 200 µg Ab, 40 µg BP | $361.00 | |||
SRp20 (C-1) Neutralizing Peptide | sc-365772 P | 100 µg/0.5 ml | $69.00 |