Spi-C Antibody (C-2): sc-514526
- Spi-C Antibody (C-2) is a mouse monoclonal IgG1 κ Spi-C antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 9-32 near the N-terminus of Spi-C of human origin
- Spi-C Antibody (C-2) is recommended for detection of Spi-C of human origin by WB, IP, IF and ELISA
- Anti-Spi-C Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-514526 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Spi-C Antibody (C-2) for WB applications. These reagents are now offered in bundles with Spi-C Antibody (C-2) (see ordering information below).
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SEE ALSO...
Spi-C Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Spi-C protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Spi-C antibody (C-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Spi-C, a member of the Ets transcription factor family, plays a crucial role in regulating gene expression, particularly in lymphoid development and function. Spi-C features a unique winged helix-turn-helix motif, known as the Ets domain, which binds to specific DNA sequences, influencing transcriptional activity. Spi-C (C-2) monoclonal antibody enables research into red pulp macrophage development, which maintains iron homeostasis and facilitates red blood cell recycling. Human Spi-C consists of 248 amino acids, localizes in the nucleus, and operates as a monomer to bind DNA. Spi-C shares 65% amino acid identity with its mouse counterpart, suggesting a conserved functional role across species. Spi-C (C-2) antibody serves as an invaluable tool for researchers studying transcriptional regulation and macrophage biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Spi-C Antibody (C-2) References:
- Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C. | Carlsson, R., et al. 2002. Gene. 299: 271-8. PMID: 12459275
- The role of ETS transcription factors in transcription and development of mouse preimplantation embryos. | Kageyama, S., et al. 2006. Biochem Biophys Res Commun. 344: 675-9. PMID: 16630543
- SPI-C and STAT6 can cooperate to stimulate IgE germline transcription. | Carlsson, R., et al. 2006. Biochem Biophys Res Commun. 344: 1155-60. PMID: 16647686
- Spi-1/PU.1 oncoprotein affects splicing decisions in a promoter binding-dependent manner. | Guillouf, C., et al. 2006. J Biol Chem. 281: 19145-55. PMID: 16698794
- Spi-C has opposing effects to PU.1 on gene expression in progenitor B cells. | Schweitzer, BL., et al. 2006. J Immunol. 177: 2195-207. PMID: 16887979
- Transgenic expression of Spi-C impairs B-cell development and function by affecting genes associated with BCR signaling. | Zhu, X., et al. 2008. Eur J Immunol. 38: 2587-99. PMID: 18792411
- Salmonella virulence factor SpiC is involved in expression of flagellin protein and mediates activation of the signal transduction pathways in macrophages. | Uchiya, KI. and Nikai, T. 2008. Microbiology (Reading). 154: 3491-3502. PMID: 18957602
- Role for Spi-C in the development of red pulp macrophages and splenic iron homeostasis. | Kohyama, M., et al. 2009. Nature. 457: 318-21. PMID: 19037245
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Spi-C Antibody (C-2) | sc-514526 | 200 µg/ml | $322.00 | |||
Spi-C Antibody (C-2): m-IgG Fc BP-HRP Bundle | sc-531151 | 200 µg Ab; 10 µg BP | $361.00 | |||
Spi-C Antibody (C-2): m-IgGκ BP-HRP Bundle | sc-524756 | 200 µg Ab, 40 µg BP | $361.00 | |||
Spi-C Antibody (C-2): m-IgG1 BP-HRP Bundle | sc-544417 | 200 µg Ab; 20 µg BP | $361.00 | |||
Spi-C Antibody (C-2) X | sc-514526 X | 200 µg/0.1 ml | $322.00 | |||
Spi-C Antibody (C-2) AC | sc-514526 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Spi-C Antibody (C-2) HRP | sc-514526 HRP | 200 µg/ml | $322.00 | |||
Spi-C Antibody (C-2) FITC | sc-514526 FITC | 200 µg/ml | $336.00 | |||
Spi-C Antibody (C-2) PE | sc-514526 PE | 200 µg/ml | $349.00 | |||
Spi-C Antibody (C-2) Alexa Fluor® 488 | sc-514526 AF488 | 200 µg/ml | $364.00 | |||
Spi-C Antibody (C-2) Alexa Fluor® 546 | sc-514526 AF546 | 200 µg/ml | $364.00 | |||
Spi-C Antibody (C-2) Alexa Fluor® 594 | sc-514526 AF594 | 200 µg/ml | $364.00 | |||
Spi-C Antibody (C-2) Alexa Fluor® 647 | sc-514526 AF647 | 200 µg/ml | $364.00 | |||
Spi-C Antibody (C-2) Alexa Fluor® 680 | sc-514526 AF680 | 200 µg/ml | $364.00 | |||
Spi-C Antibody (C-2) Alexa Fluor® 790 | sc-514526 AF790 | 200 µg/ml | $364.00 | |||
Spi-C (C-2) Neutralizing Peptide | sc-514526 P | 100 µg/0.5 ml | $69.00 |