SPCA1/2 Antibody (B-3): sc-377339
- SPCA1/2 Antibody (B-3) is a mouse monoclonal IgG2a κ SPCA1/2 antibody provided at 200 µg/ml
- raised against amino acids 711-820 mapping near the C-terminus of SPCA2 of human origin
- SPCA1/2 Antibody (B-3) is recommended for detection of SPCA1 and SPCA2 of human origin by WB, IP, IF and ELISA
- Anti-SPCA1/2 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SPCA1/2 Antibody (B-3) for WB applications. These reagents are now offered in bundles with SPCA1/2 Antibody (B-3) (see ordering information below).
SPCA1/2 Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects SPCA1/2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPCA1/2 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPCA1, encoded by the ATP2C1 gene, plays a crucial role as a calcium and manganese transport ATPase, primarily located in the Golgi apparatus. This localization helps maintain the ionic balance within the Golgi lumen, which is essential for proper protein processing and secretion. SPCA1′s interaction with SERCA2 ensures that calcium levels are tightly regulated, which is vital for various cellular functions, including muscle contraction and neurotransmitter release. Mutations in the ATP2C1 gene can lead to Hailey-Hailey disease, an autosomal dominant condition characterized by skin blistering and acantholysis, highlighting SPCA1′s importance in maintaining cellular integrity. SPCA2, encoded by the ATP2C2 gene, also resides in the Golgi apparatus and exhibits a higher enzymatic turnover rate compared to SPCA1, suggesting a specialized role in secretory cells, particularly in the gastrointestinal and respiratory tracts, as well as in glands such as the prostate, thyroid, salivary, and mammary glands.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPCA1/2 Antibody (B-3) References:
- Mutations in ATP2C1, encoding a calcium pump, cause Hailey-Hailey disease. | Hu, Z., et al. 2000. Nat Genet. 24: 61-5. PMID: 10615129
- Hailey-Hailey disease is caused by mutations in ATP2C1 encoding a novel Ca(2+) pump. | Sudbrak, R., et al. 2000. Hum Mol Genet. 9: 1131-40. PMID: 10767338
- Characterization of 16 novel human genes showing high similarity to yeast sequences. | Stanchi, F., et al. 2001. Yeast. 18: 69-80. PMID: 11124703
- Analysis of ATP2C1 gene mutation in 10 unrelated Japanese families with Hailey-Hailey disease. | Yokota, K., et al. 2002. J Invest Dermatol. 118: 550-1. PMID: 11874499
- Effect of Hailey-Hailey Disease mutations on the function of a new variant of human secretory pathway Ca2+/Mn2+-ATPase (hSPCA1). | Fairclough, RJ., et al. 2003. J Biol Chem. 278: 24721-30. PMID: 12707275
- A novel isoform of the secretory pathway Ca2+,Mn(2+)-ATPase, hSPCA2, has unusual properties and is expressed in the brain. | Xiang, M., et al. 2005. J Biol Chem. 280: 11608-14. PMID: 15677451
- The secretory pathway Ca2+/Mn2+-ATPase 2 is a Golgi-localized pump with high affinity for Ca2+ ions. | Vanoevelen, J., et al. 2005. J Biol Chem. 280: 22800-8. PMID: 15831496
- Dissection of the functional differences between human secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and 2 isoenzymes by steady-state and transient kinetic analyses. | Dode, L., et al. 2006. J Biol Chem. 281: 3182-9. PMID: 16332677
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPCA1/2 Antibody (B-3) | sc-377339 | 200 µg/ml | $322.00 | |||
SPCA1/2 Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-530013 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPCA1/2 Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-523289 | 200 µg Ab, 40 µg BP | $361.00 | |||
SPCA1/2 Antibody (B-3): m-IgG2a BP-HRP Bundle | sc-547419 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPCA1/2 Antibody (B-3) AC | sc-377339 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SPCA1/2 Antibody (B-3) HRP | sc-377339 HRP | 200 µg/ml | $322.00 | |||
SPCA1/2 Antibody (B-3) FITC | sc-377339 FITC | 200 µg/ml | $336.00 | |||
SPCA1/2 Antibody (B-3) PE | sc-377339 PE | 200 µg/ml | $349.00 | |||
SPCA1/2 Antibody (B-3) Alexa Fluor® 488 | sc-377339 AF488 | 200 µg/ml | $364.00 | |||
SPCA1/2 Antibody (B-3) Alexa Fluor® 546 | sc-377339 AF546 | 200 µg/ml | $364.00 | |||
SPCA1/2 Antibody (B-3) Alexa Fluor® 594 | sc-377339 AF594 | 200 µg/ml | $364.00 | |||
SPCA1/2 Antibody (B-3) Alexa Fluor® 647 | sc-377339 AF647 | 200 µg/ml | $364.00 | |||
SPCA1/2 Antibody (B-3) Alexa Fluor® 680 | sc-377339 AF680 | 200 µg/ml | $364.00 | |||
SPCA1/2 Antibody (B-3) Alexa Fluor® 790 | sc-377339 AF790 | 200 µg/ml | $364.00 |