Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Somatostatin Antibody (H-11) - Immunohistochemistry - Image 357909
体生长抑素抗体 (H-11): sc-74556. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示朗格汉斯小体的胞浆染色.
体生长抑素抗体 (H-11): sc-74556. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示朗格汉斯小体的胞浆染色.

Somatostatin 抗体 (H-11): sc-74556

4.5(16)
寫評論提問

说明书
  • Somatostatin抗体(H-11)是小鼠单克隆IgG1 κ, 在30篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Somatostatin的氨基酸25-116
  • 抗-Somatostatin 抗体 (H-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Somatostatin; 也和以下物种反应，包括： 和 equine, bovine, porcine and canine
  • 抗Somatostatin抗体（H-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取Somatostatin (H-11): sc-74556的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 Somatostatin 抗体 (H-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Somatostatin 抗体 (H-11) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

生长抑素抗体（H-11）是一种IgG1 κ小鼠单克隆生长抑素抗体，可通过WB, IP, IF, IHC(P)和ELISA检测小鼠、大鼠和人类来源的生长抑素。生长抑素抗体（H-11）既以非偶联抗生长抑素抗体的形式存在，也以多种偶联抗生长抑素抗体的形式存在，包括琼脂糖、辣根过氧化物酶（HRP）、biotin、 藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。生长抑素是一种调节激素，在全身表达，并通过与高亲和力的G蛋白偶联的生长抑素受体结合，抑制多种次级激素的释放。这种环状十四肽抑制许多重要激素的分泌，包括生长激素（也称为生长激素或GH）、胰岛素和胰高血糖素。在下丘脑和胰腺中均可发现生长抑素。人们认为生长抑素参与胰岛素合成的调节。激素生长抑素具有由该基因编码的单一前蛋白通过交替裂解产生的活性14个氨基酸和28个氨基酸的形式。在小脑中，生长抑素-14和生长抑素-28分别在出生和成年阶段高度表达。生长抑素影响中枢神经系统的神经传递速率以及正常细胞和致瘤细胞的增殖。编码生长抑素的基因定位于人类3号染色体q27区。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Somatostatin 抗体 (H-11) 参考文献:

  1. 在小脑颗粒细胞成熟过程中，体生长抑素和体生长抑素受体2受钙调素的相互控制。  |  Kramer, D., et al. 2005. J Neurochem. 94: 1374-83. PMID: 16000155
  2. 与 PDZ 结构域蛋白 PIST/GOPC 和 PDZK1 的相互作用调控体生长抑素受体亚型 5 的胞内排序。  |  Wente, W., et al. 2005. J Biol Chem. 280: 32419-25. PMID: 16012170
  3. 体生长抑素通过不同的受体亚型下调培养的大鼠星形胶质细胞中内氮杂卓的表达和释放。  |  Masmoudi, O., et al. 2005. J Neurochem. 94: 561-71. PMID: 16033415
  4. 在 FRTL5 细胞中，促甲状腺激素和胰岛素样生长因子-I 都会抑制脱氧核糖核酸的合成。  |  Tsuzaki, S. and Moses, AC. 1990. Endocrinology. 126: 3131-8. PMID: 1972059
  5. 体生长抑素类似物在治疗癌症中的临床应用。  |  Lamberts, SW., et al. 1990. Baillieres Clin Endocrinol Metab. 4: 29-49. PMID: 1975166
  6. 长效生长抑素类似物对实验性糖尿病大鼠晶状体氧化还原系统的影响。  |  Kunjara, S., et al. 2014. Int J Exp Pathol. 95: 95-100. PMID: 24602114
  7. 人体血浆中生长抑素的特异性放射免疫分析的开发与验证。  |  Penman, E., et al. 1979. Ann Clin Biochem. 16: 15-25. PMID: 420498
  8. 人类体生长抑素 I 基因的序列。  |  Shen, LP. and Rutter, WJ. 1984. Science. 224: 168-71. PMID: 6142531
  9. 无细胞生物合成多种前唾液腺激素：通过杂交选择和氨基末端测序确定其特征。  |  Warren, TG. and Shields, D. 1984. Biochemistry. 23: 2684-90. PMID: 6147156
  10. 通过原位杂交对人类淀粉酶, 原绒毛膜促皮质素, 体生长抑素基因和一个 DNA 片段（D3S1）进行高分辨率染色体定位。  |  Zabel, BU., et al. 1983. Proc Natl Acad Sci U S A. 80: 6932-6. PMID: 6196780
  11. 胰岛素, 胰高血糖素和促体生长素分泌细胞上的胰岛素样生长因子-I（IGF-I）和 IGF-II 功能性活性受体。  |  Fehmann, HC., et al. 1996. Metabolism. 45: 759-66. PMID: 8637452
  12. 生长激素通过其在弓状核的神经肽 Y 神经元和室周核的体生长抑素神经元上的受体作用于下丘脑，从而抑制自身分泌。  |  Minami, S., et al. 1998. Endocr J. 45 Suppl: S19-26. PMID: 9790225

订购信息

产品名称产品编号规格价格数量收藏夹

Somatostatin 抗体 (H-11)

sc-74556
200 µg/ml
$316.00

Somatostatin (H-11): m-IgG Fc BP-HRP 套装

sc-525729
200 µg Ab; 10 µg BP
$354.00

Somatostatin 抗体 (H-11) AC

sc-74556 AC
500 µg/ml, 25% agarose
$416.00

Somatostatin 抗体 (H-11) HRP

sc-74556 HRP
200 µg/ml
$316.00

Somatostatin 抗体 (H-11) FITC

sc-74556 FITC
200 µg/ml
$330.00

Somatostatin 抗体 (H-11) PE

sc-74556 PE
200 µg/ml
$343.00

Somatostatin 抗体 (H-11) Alexa Fluor® 488

sc-74556 AF488
200 µg/ml
$357.00

Somatostatin 抗体 (H-11) Alexa Fluor® 546

sc-74556 AF546
200 µg/ml
$357.00

Somatostatin 抗体 (H-11) Alexa Fluor® 594

sc-74556 AF594
200 µg/ml
$357.00

Somatostatin 抗体 (H-11) Alexa Fluor® 647

sc-74556 AF647
200 µg/ml
$357.00

Somatostatin 抗体 (H-11) Alexa Fluor® 680

sc-74556 AF680
200 µg/ml
$357.00

Somatostatin 抗体 (H-11) Alexa Fluor® 790

sc-74556 AF790
200 µg/ml
$357.00

抗体 (H-11) B

sc-74556 B
200 µg/ml
$320.00

Can I use SST Ab(H-11) on mouse? I saw some non-specific binding when I did the IHC with SSt Ab(H-11) on mouse brains

Asked by: Xiaqiu
Thank you for your question. Yes, this SST antibody is mouse, rat and human reactive. Please reach out to Technical Support if you need assistance troubleshooting your IHC results.
Answered by: Tech Service
Date published: 2019-10-09

Can sc-74556 label for both ss-14 and ss-28 on a western blot? ie would it recognise both forms that could be viewed by a difference in size?

Asked by: MW01
Thank you for your question. The expected molecular weight for this protein is ~17kDa. We do not have information about seeing the 28kDa band.
Answered by: Tech Service
Date published: 2019-03-13

Can Somatostatin (H-11): sc-74556 antibody be used to stain free floating thick (50µm) vibratome mouse brain slices, fixed overnight with 4% PFA? If so, does it require antigen retrieval? We need to use it with a fluorescent secondary antibody. 

Asked by: irini
Thank you for your question. We have not tested this antibody specifically on free floating tissue sections. However, we do recommend the antibody for immunohistochemistry on formalin-fixed tissue sections. If you are using 4% PFA for fixation, we do recommend antigen retrieval. Our preferred method is sodium citrate buffer (pH 6.0) with heat treatment, but that may not be suitable for your free floating tissue sections. We would recommend testing different antigen retrieval methods with the antibody in your application to determine the optimal results.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-11

Can Somatostatin (H-11): sc-74556 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Somatostatin (H-11): sc-74556 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-17
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_74556, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from SOM H-11Compre este producto el año pasado y funciono bien.
Date published: 2023-02-27
Rated 4 out of 5 by from BrainH-11 works well in the highly fixed brain (PFA perfusion + 1 hour post fixation in PFA)
Date published: 2023-01-23
Rated 5 out of 5 by from works very well for IHC-P of porcine pancreataAlthough it have to be used in a less diluted concentration, this antibody works very well in IHC-P of formalin fixed porcine pancreatic tissue.
Date published: 2018-07-08
Rated 5 out of 5 by from Good antibody for mouse brain frozen sliceWe used this antibody for immunofluorescence staining.
Date published: 2018-05-04
Rated 5 out of 5 by from ottimo su sezioni di encefalo di topo (vibratomo)ho ricevuto questo free sample un mese fa e mi ha dato ottimi risultati su sezioni di encefalo di topo perfuso con paraformaldeide al 4% e tagliate mediante l'uso di un vibratomo in sezioni di 50 micron
Date published: 2018-02-20
Rated 4 out of 5 by from Good signal, low background, standard protocolI tried this as a sample and I can recommend it. I used standard protocol for DAB staining and it worked right away. I did not use any amplification kit, just very basic chemicals and DAB in powder. So I believe the signal still can be improved a lot if protocol is optimized. Protocol notes: - antigen retrieval in citrate buffer for 10 minutes - 30 min pre-incubation with 1% H2O2 - blocking for 1 h in PBS + 0.15% Triton + 5% BSA + 5% goat serum - incubation with primary o/n (in PBS + 0.15% Triton + 2% goat serum) - incubation with secondary 2 hrs in the same solution - washing always in PBS + 0.15% Triton
Date published: 2018-01-02
Rated 5 out of 5 by from somatostatin sc74556This is the very good antibody for somatostatin in the brain
Date published: 2017-12-21
Rated 5 out of 5 by from IHC/IF staining on FFPE pig pancreasantibody worked fine in the application of immunohistochemical staining on formalin-fixed paraffin embedded pancreatic porcine tissue
Date published: 2017-09-11
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_74556, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
Somatostatin 抗体 (H-11) is rated 4.5 out of 5 by 16.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_74556, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 85ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。