Date published: 2025-9-6

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SNAP47 抗体 (D-11): sc-514428

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说明书
  • SNAP47 抗体 D-11 是小鼠单克隆 IgG1 κ,SNAP47抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列290-442位于human物种的SNAP47的C-terminus附近
  • SNAP47 抗体 (D-11) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 SNAP47
  • 抗SNAP47抗体(D-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SNAP47 (D-11): sc-514428免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是SNAP47 Antibody (D-11) for WB applications. 的首选辅助检测试剂,这些试剂现与SNAP47 Antibody (D-11) 打包提供(请参阅下面的订购信息)。

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関連項目

SNAP47抗体(D-11)是一种IgG1 κ小鼠单克隆SNAP47抗体(也称为SNAP47抗体),可通过WB、IP、IF和ELISA检测人源SNAP47蛋白。SNAP47抗体(D-11)既有非偶联形式的抗SNAP47抗体,也有多种偶联形式的抗SNAP47抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在真核细胞中,高尔基体从内质网接收新合成的蛋白质,并在共价修饰后将其运送到细胞内的目的地。对于膜定向蛋白质,这一过程被认为是通过囊泡运输进行的。正确的囊泡运输由囊泡相关的SNAREs(v-SNAREs)与目标膜上的SNAREs(t-SNAREs)的特定配对决定。然后,这个复合物招募可溶性NSF附着蛋白(SNAPs)和N-乙基马来酰亚胺敏感因子(NSF)以形成高度稳定的SNAP受体(SNARE)复合物。SNARE复合物的形成将囊泡和目标膜拉在一起,并可能提供能量来驱动脂质双层的融合。SNAP47(突触体相关蛋白47),也称为附睾腔蛋白170,是一种含有464个氨基酸的蛋白质,普遍表达,在神经组织中的表达水平最高。由于选择性剪接事件,产生了四种SNAP47异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SNAP47 抗体 (D-11) 参考文献:

  1. 鉴定 SNAP-47,一种无处不在的新型 Qbc-SNARE 表达。  |  Holt, M., et al. 2006. J Biol Chem. 281: 17076-17083. PMID: 16621800
  2. 细胞中的膜融合:分子机制和机制。  |  Leabu, M. 2006. J Cell Mol Med. 10: 423-7. PMID: 16796809
  3. 分泌机制的核心蛋白  |  Lang, T. and Jahn, R. 2008. Handb Exp Pharmacol. 107-27. PMID: 18064413
  4. 膜融合过程中 SNARE 的组装和解体。  |  Jena, BP. 2008. Methods Cell Biol. 90: 157-82. PMID: 19195550
  5. 对肉毒杆菌神经毒素进行工程改造,以扩大治疗范围。  |  Chen, S. and Barbieri, JT. 2009. Proc Natl Acad Sci U S A. 106: 9180-4. PMID: 19487672
  6. 神经元 SNARE 复合物向膜的螺旋延伸。  |  Stein, A., et al. 2009. Nature. 460: 525-8. PMID: 19571812
  7. 膜融合:SNARE 和钙的作用。  |  Jena, BP. 2009. Protein Pept Lett. 16: 712-7. PMID: 19601899
  8. SNARE 介导的膜融合的跨膜域决定因素。  |  Fdez, E., et al. 2010. J Cell Sci. 123: 2473-80. PMID: 20571052
  9. 外细胞 SNARE 对哺乳动物细胞重组蛋白生产的不同影响。  |  Peng, RW., et al. 2011. Biotechnol Bioeng. 108: 611-20. PMID: 21246508
  10. SNAP47 与 ATG14 相互作用,促进 VP1 连接和 CVB3 传播。  |  Xiang, P., et al. 2021. Cells. 10: PMID: 34440910
  11. Circ-SNAP47(hsa_circ_0016760)和miR-625-5p是WEE1的调控因子,它们通过ceRNA途径调控耐受DDP的NSCLC细胞的化疗抗性, 生长和侵袭。  |  Zou, C., et al. 2022. Environ Toxicol. 37: 224-236. PMID: 34664776
  12. STX17-SNAP47-VAMP7/VAMP8 复合物是介导自噬体与溶酶体融合的默认 SNARE 复合物。  |  Jian, F., et al. 2024. Cell Res. 34: 151-168. PMID: 38182888

订购信息

产品名称产品编号规格价格数量收藏夹

SNAP47 抗体 (D-11)

sc-514428
200 µg/ml
$316.00

SNAP47 (D-11): m-IgG Fc BP-HRP 套装

sc-527900
200 µg Ab; 10 µg BP
$354.00

SNAP47 (D-11): m-IgG1 BP-HRP 套装

sc-533273
200 µg Ab; 20 µg BP
$354.00

SNAP47 抗体 (D-11) AC

sc-514428 AC
500 µg/ml, 25% agarose
$416.00

SNAP47 抗体 (D-11) HRP

sc-514428 HRP
200 µg/ml
$316.00

SNAP47 抗体 (D-11) FITC

sc-514428 FITC
200 µg/ml
$330.00

SNAP47 抗体 (D-11) PE

sc-514428 PE
200 µg/ml
$343.00

SNAP47 抗体 (D-11) Alexa Fluor® 488

sc-514428 AF488
200 µg/ml
$357.00

SNAP47 抗体 (D-11) Alexa Fluor® 546

sc-514428 AF546
200 µg/ml
$357.00

SNAP47 抗体 (D-11) Alexa Fluor® 594

sc-514428 AF594
200 µg/ml
$357.00

SNAP47 抗体 (D-11) Alexa Fluor® 647

sc-514428 AF647
200 µg/ml
$357.00

SNAP47 抗体 (D-11) Alexa Fluor® 680

sc-514428 AF680
200 µg/ml
$357.00

SNAP47 抗体 (D-11) Alexa Fluor® 790

sc-514428 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with SNAP47 (D-11): sc-514428 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
  • y_2025, m_8, d_28, h_5CST
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Rated 5 out of 5 by from Great choice for Western Blot of SNAP47This antibody detects over-expressed human SNAP47 (isoform 4) in transfected lysate sc-115005 and endogenous SNAP47 (isoform 1) in Jurkat whole cell lysate. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from well doneit works well in sk-hep-1 cell by 1:1000 in WB,thanks!
Date published: 2017-11-04
Rated 5 out of 5 by from Antibody works!Antibody detected a 47 kDa protein after immunoprobing overnight (1:1000 dilution in 2.5% TBST). Signal seems to be sensitive to my experimental treatments as well. Hoping to further confirm with siSNAP47 experiments.
Date published: 2017-08-25
Rated 4 out of 5 by from not for hydraTested in IHC in hydra vulgaris and did not react, as expected. Did not try in any other species
Date published: 2017-08-01
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SNAP47 抗体 (D-11) is rated 4.6 out of 5 by 5.
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