SMP30抗体 (G-10) 推荐用于 mouse, rat 和 human 来源的SMP30 WB, IP, IF, IHC(P) 和 ELISA检测

SMP30 抗体 (G-10): sc-374019

SMP30抗体 (G-10) is rated 4.8 out of 5 by 5.
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说明书

    • SMP30抗体(G-10)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于human物种的SMP30的内部区域内的氨基酸247-279之间
    • 抗-SMP30 抗体 (G-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SMP30
    • 抗-SMP30 抗体 (G-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取SMP30 (G-10): sc-374019免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for SMP30 Antibody (G-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with SMP30 Antibody (G-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the different between SMP30 Antibody (17) and SMP30 Antibody (G-10)? I would like to detect the SMP30 expression in liver and kidney of rats in both western blot and immunohistochemistry.  So, which product should be suitable for this detection?

Asked by: Primates
Thank you for your question. These are two different mouse monoclonal antibodies with distinct epitopes, both recognizing SMP30. Both antibodies are suitable for your intended research applications.
Answered by: Technical Support
Date published: 2019-01-26

这支抗体能检测smp30蛋白的其他亚型吗?WB、IP、IF、elisa、IHC、FCM真的都可以做吗?能否送一个试用装给我用一下?

Asked by: sunshine666
感谢您的提问。官网上说明的我们都是提供质保的,关于试用装的申请,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

I used this Ab (sc-374019) to stain FFPE kidney sections (Rat) following  IF & IHC protocols but there was no staining (Antigen retrieval with 10 mM sodium citrate buffer, pH 6.0 at 100 °C,10 min).Do you recommend any special treatment for this antibody?

Asked by: GBar
Thank you for your question. For the IHC(P) data shown on our website using this antibody, SMP30 (G-10): sc-374019, we also used the same antigen retrieval method of sodium citrate buffer with heat treatment. If you have not seeing any staining, we would recommend using the primary antibody at the starting dilution of 1:50. A link to our complete recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you need assistance in troubleshooting the antibody, please contact our Technical Service Department. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-06-29

What application is the blocking peptide sc-374019 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from good IHC staining I got good IHC staining result with DAB in mouse liver
Date published: 2019-11-22
Rated 5 out of 5 by from Great It is reactive with mouse, rat, and human, bands were clear.
Date published: 2017-05-16
Rated 5 out of 5 by from Wise choice in antibody... This is a strong SMP30 clone, clean specific and reliable. Great addition to any lab working with SMP30!
Date published: 2017-02-02
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic and Beautiful immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Great Western Blot data of SMP30 expression Great Western Blot data of SMP30 expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2013-02-08
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